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Revision as of 09:37, 22 September 2010 (view source) Hildegard (Talk | contribs) ← Older edit		Latest revision as of 09:49, 22 September 2010 (view source) Hildegard (Talk | contribs)
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	Hilde:		Hilde:
-	Fastdigest cutting: pduABJKNTUV(E,X)	+	PCR test of pduN (N-r, N-f) Model: NTUV plm.-1/-2/-3; NTUV plm.2 No.1-2-3-4; ABJKNTUV#2-#9-#5;TUV5(AD);NULL
-	Ligation of Promotor-pduABJKNTUV	+	PCR test of pduN (N-r, N-f) Model: NTUV plm.-2/-3; NTUV plm.2 No.4-1-2-3; ABJKNTUV#2-#9-#5;TUV5(AD);N(+);NULL
-	Transformation of ligation produce	+	Fastdigest cutting: pduN (Insert,X,P) & Promotor (Vector,S,P)
-	Coating of Promotor-pduABJKNTUV onto Cl+ plate	+	miniprep: NU1-8;JK1-1-8;RBS1-2
		+
		+	Sending to sequence test: JK1-4-7;NU1-4-7;RBS1-2

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Latest revision as of 09:49, 22 September 2010

Jocelyn:

colony PCR to test pduN.

colony PCR before miniprep ×40

double digest of ABJKTUV in promoter and pduN.

storage of bacterial ×36

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Hilde:

PCR test of pduN (N-r, N-f) Model: NTUV plm.-1/-2/-3; NTUV plm.2 No.1-2-3-4; ABJKNTUV#2-#9-#5;TUV5(AD);NULL

PCR test of pduN (N-r, N-f) Model: NTUV plm.-2/-3; NTUV plm.2 No.4-1-2-3; ABJKNTUV#2-#9-#5;TUV5(AD);N(+);NULL

Fastdigest cutting: pduN (Insert,X,P) & Promotor (Vector,S,P)

miniprep: NU1-8;JK1-1-8;RBS1-2

Sending to sequence test: JK1-4-7;NU1-4-7;RBS1-2