Team:USTC/Notebook/Meetings/16 September 2010
From 2010.igem.org
(Difference between revisions)
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Hilde: | Hilde: | ||
- | + | PCR test of pduN (N-r, N-f) Model: NTUV plm.-1/-2/-3; NTUV plm.2 No.1-2-3-4; ABJKNTUV#2-#9-#5;TUV5(AD);NULL | |
- | + | PCR test of pduN (N-r, N-f) Model: NTUV plm.-2/-3; NTUV plm.2 No.4-1-2-3; ABJKNTUV#2-#9-#5;TUV5(AD);N(+);NULL | |
- | + | Fastdigest cutting: pduN (Insert,X,P) & Promotor (Vector,S,P) | |
- | + | miniprep: NU1-8;JK1-1-8;RBS1-2 | |
+ | |||
+ | Sending to sequence test: JK1-4-7;NU1-4-7;RBS1-2 |
Latest revision as of 09:49, 22 September 2010
Jocelyn:
colony PCR to test pduN.
colony PCR before miniprep ×40
double digest of ABJKTUV in promoter and pduN.
storage of bacterial ×36
Hilde:
PCR test of pduN (N-r, N-f) Model: NTUV plm.-1/-2/-3; NTUV plm.2 No.1-2-3-4; ABJKNTUV#2-#9-#5;TUV5(AD);NULL
PCR test of pduN (N-r, N-f) Model: NTUV plm.-2/-3; NTUV plm.2 No.4-1-2-3; ABJKNTUV#2-#9-#5;TUV5(AD);N(+);NULL
Fastdigest cutting: pduN (Insert,X,P) & Promotor (Vector,S,P)
miniprep: NU1-8;JK1-1-8;RBS1-2
Sending to sequence test: JK1-4-7;NU1-4-7;RBS1-2