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Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test27agosto
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| - | <html><p align="center"><font size="5"><b> | + | <html><p align="center"><font size="5"><b>AUGUST, 27TH</b></font></p></html><hr><br> |
==PLATE== | ==PLATE== | ||
<br> | <br> | ||
| - | [[Image: | + | [[Image:UNIPV_Pavia_piastra27agosto2.png| 620px | center ]] |
<br> | <br> | ||
| + | |||
==EXPERIMENT DESCRIPTION== | ==EXPERIMENT DESCRIPTION== | ||
| - | ==RESULTS ( | + | |
| + | ===Motivation=== | ||
| + | This experiment was performed in LB to check bacterial growth and RFP rate synthesis in order to evaluate the R.P.U. ([[Team:UNIPV-Pavia/Parts/Characterization#Data analysis for RPU evaluation|see Protocol]]) of the promoters from Anderson's collection in high copy number plasmids. | ||
| + | |||
| + | ===Methods=== | ||
| + | Accordign to [[Team:UNIPV-Pavia/Parts/Characterization#Microplate reader experiments for constitutive promoters (R.P.U. evaluation) - Protocol #2|Protocol 2]] the experiment was performed in LB on the following parts: | ||
| + | |||
| + | * <partinfo>BBa_J23100</partinfo> | ||
| + | * <partinfo>BBa_J23101</partinfo> | ||
| + | * <partinfo>BBa_J23105</partinfo> | ||
| + | * <partinfo>BBa_J23106</partinfo> | ||
| + | * <partinfo>BBa_J23110</partinfo> | ||
| + | * <partinfo>BBa_J23114</partinfo> | ||
| + | * <partinfo>BBa_J23116</partinfo> | ||
| + | * <partinfo>BBa_J23118</partinfo> | ||
| + | * <partinfo>BBa_B0031</partinfo> (negative control) | ||
| + | |||
| + | ==RESULTS== | ||
| + | <br> | ||
| + | [[Image:UNIPV_Pavia_risHCLB.png| 620px | center ]] | ||
| + | <br> | ||
| + | |||
| + | It is possible to split the promoters into 3 groups: strong promoters (<partinfo>BBa_J23100</partinfo> and <partinfo>BBa_J23101</partinfo>), medium strength promoters(<partinfo>BBa_J23118</partinfo> and <partinfo>BBa_J23110</partinfo>) and weak promoters (the other ones). It is notable that <partinfo>BBa_J23110</partinfo> resulted stronger than <partinfo>BBa_J23118</partinfo>. | ||
| + | |||
| + | {| border='1' align='center' | ||
| + | || Promoter || R.P.U. (average) || std | ||
| + | |- | ||
| + | || <partinfo>BBa_J23100</partinfo> || 0.989 || 0.025 | ||
| + | |- | ||
| + | || <partinfo>BBa_J23101</partinfo> || 1 || - | ||
| + | |- | ||
| + | || <partinfo>BBa_J23105</partinfo> || 0.071 || 0.004 | ||
| + | |- | ||
| + | || <partinfo>BBa_J23106</partinfo> || 0.122 || 0.008 | ||
| + | |- | ||
| + | || <partinfo>BBa_J23110</partinfo> || 0.583 || 0.016 | ||
| + | |- | ||
| + | || <partinfo>BBa_J23114</partinfo> || 0.037 || 0.006 | ||
| + | |- | ||
| + | || <partinfo>BBa_J23116</partinfo> || 0.043 || 0.009 | ||
| + | |- | ||
| + | || <partinfo>BBa_J23118</partinfo> || 0.341 || 0.009 | ||
| + | |} | ||
<!-- table previous next test --> | <!-- table previous next test --> | ||
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| - | + | {{UNIPV-Pavia/Menu_measurments}} | |
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Latest revision as of 08:42, 23 October 2010
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