Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test18agosto

From 2010.igem.org

(Difference between revisions)

Latest revision as of 21:10, 25 October 2010

AUGUST, 18TH

PLATE

EXPERIMENT DESCRIPTION

Motivation

The experiment was performed to evaluate the initiation transcription point (in term of absorbance) for self-inducible devices.

Methods

The experiment was performed in LB according to Protocol 1 on the following measurement parts:

<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7)
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8)
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12)
<partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name I14-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> (wiki name I15-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name I16-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name I17-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name I18-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name I19-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
<partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> (negative control)

RESULTS

Data were analyzed following the data analysis procedure to evaluate the ODstart. The plots correpsoning to the parts tested are reported:

The ODstart are reported in the table below:

Part	ODstart
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo>	0.1655
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo>	-
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo>	-
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo>	0.5421
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo>	0.4837
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo>	0.1503
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo>	0.3587
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo>	-
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo>	0.1729
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo>	-
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo>	-

Tecan Test

@@ Line 11: / Line 11: @@
 <td valign="top">
 <table border="0" align="center" width="100%"><tr><td align="justify" valign="top" style="padding:20px">
-<html><p align="center"><font size="5"><b>13° TEST</b></font><br><br><font size="4">[AUGUST, 18TH]</font></p></html><hr><br>
+<html><p align="center"><font size="5"><b>AUGUST, 18TH</b></font></p></html><hr><br>
 ==PLATE==
 <br>
@@ Line 17: / Line 17: @@
 <br>
 ==EXPERIMENT DESCRIPTION==
-==RESULTS (?)==
+===Motivation===
+The experiment was performed to evaluate the initiation transcription point (in term of absorbance) for self-inducible devices.
+===Methods===
+The experiment was performed in LB according to [[Team:UNIPV-Pavia/Parts/Characterization#Microplate reader experiments for self-inducible promoters - Protocol #1|Protocol 1]] on the following measurement parts:
+* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7)
+* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8)
+* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
+* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
+* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12)
+* <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
+* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
+* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name I14-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
+* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> (wiki name I15-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
+* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name I16-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
+* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name I17-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
+* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name I18-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
+* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name I19-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
+* <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> (negative control)
+==RESULTS==
+Data were analyzed following the [[Team:UNIPV-Pavia/Parts/Characterization#Data analysis for self-inducible promoters (initiation-treshold determination)| data analysis procedure]] to evaluate the ODstart. The plots correpsoning to the parts tested are reported:
+[[Image:UNIPV_Pavia_FluoVSODHC.png| 620px | center ]]
+[[Image:UNIPV_Pavia_FluoVSODCo.png| 620px | center ]]
+The ODstart are reported in the table below:
+{| border='1' align='center'
+|| Part || ODstart
+|-
+|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> || 0.1655
+|-
+|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> || -
+|-
+|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> || -
+|-
+|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> || 0.5421
+|-
+|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> || 0.4837
+|-
+|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> || 0.1503
+|-
+|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> || 0.3587
+|-
+|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> || -
+|-
+|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> || 0.1729
+|-
+|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> || -
+|-
+|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> || -
+|}
 <!-- table previous next test -->
@@ Line 35: / Line 90: @@
 <td align="left" valign="top" width="15%">
-<table border="0" width="100%" height="100%">
+{{UNIPV-Pavia/Menu_measurments}}
-<tr align="right"><td height="15">
-[[Team:UNIPV-Pavia/Material_Methods/Measurements|MEASUREMENTS]]
-</td></tr>
-<tr align="right"><td height="15">
-[[Team:UNIPV-Pavia/Material_Methods/Measurements/Tecan|Tecan Test]]
-</td></tr>
-</table>
 </td>
 </table>