Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test27agosto

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<html><p align="center"><font size="5"><b>15° TEST</b></font><br><br><font size="4">[AUGUST, 27TH]</font></p></html><hr><br>
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<html><p align="center"><font size="5"><b>AUGUST, 27TH</b></font></p></html><hr><br>
==PLATE==
==PLATE==
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[[Image:UNIPV_Pavia_piastra27agosto.png| 620px | center ]]
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[[Image:UNIPV_Pavia_piastra27agosto2.png| 620px | center ]]
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==EXPERIMENT DESCRIPTION==
==EXPERIMENT DESCRIPTION==
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==RESULTS (?)==
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===Motivation===
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This experiment was performed in LB to check bacterial growth and RFP rate synthesis in order to evaluate the R.P.U. ([[Team:UNIPV-Pavia/Parts/Characterization#Data analysis for RPU evaluation|see Protocol]]) of the promoters from Anderson's collection in high copy number plasmids.
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===Methods===
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Accordign to [[Team:UNIPV-Pavia/Parts/Characterization#Microplate reader experiments for constitutive promoters (R.P.U. evaluation) - Protocol #2|Protocol 2]] the experiment was performed in LB on the following parts:
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* <partinfo>BBa_J23100</partinfo>
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* <partinfo>BBa_J23101</partinfo>
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* <partinfo>BBa_J23105</partinfo>
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* <partinfo>BBa_J23106</partinfo>
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* <partinfo>BBa_J23110</partinfo>
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* <partinfo>BBa_J23114</partinfo>
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* <partinfo>BBa_J23116</partinfo>
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* <partinfo>BBa_J23118</partinfo>
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* <partinfo>BBa_B0031</partinfo> (negative control)
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==RESULTS==
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<br>
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[[Image:UNIPV_Pavia_risHCLB.png| 620px | center ]]
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<br>
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It is possible to split the promoters into 3 groups: strong promoters (<partinfo>BBa_J23100</partinfo> and <partinfo>BBa_J23101</partinfo>), medium strength promoters(<partinfo>BBa_J23118</partinfo> and <partinfo>BBa_J23110</partinfo>) and  weak promoters (the other ones). It is notable that <partinfo>BBa_J23110</partinfo> resulted stronger than <partinfo>BBa_J23118</partinfo>.
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{| border='1' align='center'
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|| Promoter || R.P.U. (average) || std
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|-
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|| <partinfo>BBa_J23100</partinfo> || 0.989 || 0.025
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|-
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|| <partinfo>BBa_J23101</partinfo> || 1 || -
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|-
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|| <partinfo>BBa_J23105</partinfo> || 0.071 || 0.004
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|-
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|| <partinfo>BBa_J23106</partinfo> || 0.122 || 0.008
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|-
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|| <partinfo>BBa_J23110</partinfo> || 0.583 || 0.016
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|-
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|| <partinfo>BBa_J23114</partinfo> || 0.037 || 0.006
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|-
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|| <partinfo>BBa_J23116</partinfo> || 0.043 || 0.009
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|-
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|| <partinfo>BBa_J23118</partinfo> || 0.341 || 0.009
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|}
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<td align="left" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test14|Previous Test]]</td>
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<td align="left" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test20agosto|Previous Test]]</td>
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<td align="right" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test16| Next Test]]</td>
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<td align="right" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test1settembre| Next Test]]</td>
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{{UNIPV-Pavia/Menu_measurments}}
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[[Team:UNIPV-Pavia/Material_Methods/Measurements|MEASUREMENTS]]
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[[Team:UNIPV-Pavia/Material_Methods/Measurements/Tecan|Tecan Test]]
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Latest revision as of 08:42, 23 October 2010
Home
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AUGUST, 27TH


PLATE


UNIPV Pavia piastra27agosto2.png


EXPERIMENT DESCRIPTION

Motivation

This experiment was performed in LB to check bacterial growth and RFP rate synthesis in order to evaluate the R.P.U. (see Protocol) of the promoters from Anderson's collection in high copy number plasmids.

Methods

Accordign to Protocol 2 the experiment was performed in LB on the following parts:

  • <partinfo>BBa_J23100</partinfo>
  • <partinfo>BBa_J23101</partinfo>
  • <partinfo>BBa_J23105</partinfo>
  • <partinfo>BBa_J23106</partinfo>
  • <partinfo>BBa_J23110</partinfo>
  • <partinfo>BBa_J23114</partinfo>
  • <partinfo>BBa_J23116</partinfo>
  • <partinfo>BBa_J23118</partinfo>
  • <partinfo>BBa_B0031</partinfo> (negative control)

RESULTS


UNIPV Pavia risHCLB.png


It is possible to split the promoters into 3 groups: strong promoters (<partinfo>BBa_J23100</partinfo> and <partinfo>BBa_J23101</partinfo>), medium strength promoters(<partinfo>BBa_J23118</partinfo> and <partinfo>BBa_J23110</partinfo>) and weak promoters (the other ones). It is notable that <partinfo>BBa_J23110</partinfo> resulted stronger than <partinfo>BBa_J23118</partinfo>.

Promoter R.P.U. (average) std
<partinfo>BBa_J23100</partinfo> 0.989 0.025
<partinfo>BBa_J23101</partinfo> 1 -
<partinfo>BBa_J23105</partinfo> 0.071 0.004
<partinfo>BBa_J23106</partinfo> 0.122 0.008
<partinfo>BBa_J23110</partinfo> 0.583 0.016
<partinfo>BBa_J23114</partinfo> 0.037 0.006
<partinfo>BBa_J23116</partinfo> 0.043 0.009
<partinfo>BBa_J23118</partinfo> 0.341 0.009



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