Team:TU Delft/Project/solubility

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Latest revision as of 18:16, 27 October 2010

Solubility

Spilled oil spreads rapidly in the environment. However, the hydrocarbons in the oil can't dissolve in the water and will remain on the water's surface or adhere to soil particles. Hydrocarbons such as octane will only reach a mole fraction of about 1.5 × 10^-7 in water. This low solubility makes microbiological degradation challenging since microorganisms will reside preferentially in the water phase. Nevertheless, a wide range of microbes including bacteria, fungi and yeasts are able to consume hydrocarbons, because evolution has equipped them with a variety of systems to improve the mass transfer from the hydrophobic phase.

It has been found that these microorganisms are capable to produce biosurfactants. This are proteins that increase the surface area of hydrophobic contaminants in water and thus increase their aqueous solubility and consequently their microbial degradation. The biosurfactants or emusifiers also enable the organisms to increase their hydrocarbon uptake, which then can be converted more efficiently into potentially valuable products. These emulsifiers are promising reagents for oil extraction from sands making the process cheaper and more sustainable.

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-=Hydrocarbon Solubility=
+{{Team:TU_Delft/frame_check}}
+__NOTOC__
+==Solubility==
+[[Image:TUDelft_Emulsification.png|300px|right]]
+Spilled oil spreads rapidly in the environment. However, the hydrocarbons in the oil can't dissolve in the water and will remain on the water's surface or adhere to soil particles. Hydrocarbons such as octane will only reach a mole fraction of about 1.5 × 10<sup>-7</sup> in water. This low solubility makes microbiological degradation challenging since microorganisms will reside preferentially in the water phase. Nevertheless, a wide range of microbes including bacteria, fungi and yeasts are able to consume hydrocarbons, because evolution has equipped them with a variety of systems to improve the mass transfer from the hydrophobic phase.
-==Introduction==
+It has been found that these microorganisms are capable to produce biosurfactants. This are proteins that increase the surface area of hydrophobic contaminants in water and thus increase their aqueous solubility and consequently their microbial degradation. The biosurfactants or emusifiers also enable the organisms to increase their hydrocarbon uptake, which then can be converted more efficiently into potentially valuable products. These emulsifiers are promising reagents for oil extraction from sands making the process cheaper and more sustainable.
-Before our engineered bugs can start degrading alkanes, they first have to be able to get it in the cell. The problem is that '''oil hardly dissolves''' in water. So what we need is just a little drop of '''soap'''. But wait a minute, doesn't soap usually kill cells?
-* [[Team:TU_Delft/Project/solubility/parts|Parts]]
+<html><center><img src="https://static.igem.org/mediawiki/2010/0/00/TU_Delft_project_navigation.jpg" usemap="#projectnavigation" border="0" /></center><map id="projectnavigation" name="projectnavigation"><area shape="rect" alt="Characterization" title="" coords="309,3,591,45" href="https://2010.igem.org/Team:TU_Delft#page=Project/solubility/characterization" target="" /><area shape="rect" alt="Results" title="" coords="609,3,891,44" href="https://2010.igem.org/Team:TU_Delft#page=Project/solubility/results" target="" /><area shape="rect" alt="Parts" title="" coords="9,3,290,44" href="https://2010.igem.org/Team:TU_Delft#page=Project/solubility/parts" target="" /></map></html>
-* [[Team:TU_Delft/Project/solubility/characterization|Characterization]]
-* [[Team:TU_Delft/Project/solubility/results|Results and Conclusions]]
-==Solubility Project Abstract==
-[[Image:TU_Delft_oil_in_water.jpg|thumb|right|Oil and water don't mix very well. [http://www.flickr.com/photos/pathfinderlinden/4709654036/sizes/l/in/photostream/ Photo by John E. Lester]]]Spilled oil spreads rapidly in the environment. However, the hydrocarbons in the oil can't dissolve in the water and will remain on the water's surface or adhere to soil particles (1). A hydrocarbons such as octane will only reach a mole fraction of about 1.5 × 10-7 in water (2). This low solubility makes microbiological degradation challenging seeing as microorganisms will reside in the water phase. Still a wide range of microbes including bacteria, fungi and yeasts are able to consume hydrocarbons (3), for evolution has equipped them with a variety of systems to improve the mass transfer from the hydrophobic phase.
-These microorganisms are producing biosurfactants. These proteins increase the surface area of hydrophobic contaminants in soil or water and thus increase their aqueous solubility and consequently their microbial degradation (1). The biosurfactants also enables the organisms to increase the hydrocarbon uptake.
-The natural oil degrading bacterium Acinetobacter radioresistens secretes a complex of proteins and polysaccharides that have emulsifying capacity (4). One of the well-known proteins with emulsification activity is [[Team:TU_Delft/Project/solubility/alna|AlnA]]. Even when this protein is produced in another organism like ''Escherichia coli'' it still works as an emulsifier (4-5).
-To make this specific property available as an interchangeable part, we [[Team:TU_Delft/Project/solubility/parts|designed and created the AlnA BioBrick]]. We also developed an universal emulsification assay, which demonstrated that modified ''E. coli'' cells with our BioBrick had emulsifying activity of ?  The expressed AlnA was highly effective in solubilizing octane, ca.? g per mg of protein, corresponding to ? molecules of octane per molecule of protein. Unmodified ''E. coli'' bacteria had no significant emulsifying or octane-solubilizing activity. The inducible AlnA protein BioBrick is now readily available from the registry.
-'''References'''
-#'''Karanth, N.G.K., Deo, P.G. and Veenanadig, N.K.''' (1999) Microbial production of biosurfactant and their importance. ''Ferment. Sci. Technol.'' 77:116-126.
-#'''Sarraute, S., Delepine, H., Costa Gomes, M.F. and Majer, V.''' (2004) Aqueous solubility, Henry's law constants and air/water partition coefficients of n-octane and two halogenated octanes. ''Chemosphere.'' 57:1543-1551.
-#'''Head, I.M., Jones, D.M. and Roling, W.F.''' (2006) Marine microorganisms make a meal of oil. ''Nature Reviews Microbiology.'' 4:173-182.
-#'''Walzer, G., Rosenberg, E. and Ron, E.Z.''' (2006) The Acinetobacter outer membrane protein A (OmpA) is a secreted emulsifier. ''Environmental Microbiology.'' 8:1026-1032.
-#'''Toren, A., Segal, G., Ron, E.Z. and Rosenberg, E.''' (2002) Structure--function studies of the recombinant protein bioemulsifier AlnA. ''Environmental Microbiology.'' 4:257-261.