Team:UC Davis/protocols/hydration.html

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<a name="extranotes"><h1>Extra Notes</h1></a>
<a name="extranotes"><h1>Extra Notes</h1></a>
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None.
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Part locations can be looked up on <a href="http://www.partsregistry.org" class="help">Parts Registry.</a><p>
<a name="procedure"><h1>Procedure</h1></a>
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<li>Keep stock (undiluted) DNA at -80°C. </li>
<li>Keep stock (undiluted) DNA at -80°C. </li>
<li>Keep diluted DNA at -20°C.</li>
<li>Keep diluted DNA at -20°C.</li>
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Latest revision as of 19:59, 10 September 2010

Hydration

Materials

You will need:

  • Sterilized milliQ water
  • Microcentrifuge tubes

Extra Notes

Part locations can be looked up on Parts Registry.

Procedure

  • Find the plate and well with the desired DNA. Use a pipette tip to open well.
  • Add 10μL of milliQ water into the well.
  • Wait 10 minutes.
  • Place 10μL into microcentrifuge tube. This will be the stock DNA
  • In a fresh new microcentrifuge tube, create a 50% dilution by aliquotting 3μL of stock DNA and 3μL milliQ water.
  • Keep stock (undiluted) DNA at -80°C.
  • Keep diluted DNA at -20°C.

Purpose

To extract DNA from registry distribution plates and keeping stocks of them.

References
We would like to take a moment to thank all of our sponsors for their very generous donations, as we could not have done this without your help!

We would also like to thank and acknowledge:
Our Advisors
Marc Facciotti
Ilias Tagkopoulos
Technical Guidance
David Larsen
Andrew Yao
Visiting iGEMer
Jia Li of Zhejiang University (TEAM ZJU-China)
cI Promoter Screen
Drew Endy - Stanford
Thomas Schneider - NIH
Want to sponsor us? Send an email to mtfacciotti@ucdavis.edu to discuss various ways you can help! :)

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