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Materials
You will need:
- Sterilized milliQ water
- Microcentrifuge tubes
Extra Notes
Part locations can be looked up on Parts Registry.
Procedure
- Find the plate and well with the desired DNA. Use a pipette tip to open well.
- Add 10μL of milliQ water into the well.
- Wait 10 minutes.
- Place 10μL into microcentrifuge tube. This will be the stock DNA
- In a fresh new microcentrifuge tube, create a 50% dilution by aliquotting 3μL of stock DNA and 3μL milliQ water.
- Keep stock (undiluted) DNA at -80°C.
- Keep diluted DNA at -20°C.
Purpose
To extract DNA from registry distribution plates and keeping stocks of them.
References
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We would like to take a moment to thank all of our sponsors for their very generous donations, as we could not have done this without your help!
We would also like to thank and acknowledge:
Our Advisors
Marc Facciotti
Ilias Tagkopoulos
Technical Guidance
David Larsen
Andrew Yao
Visiting iGEMer
Jia Li of Zhejiang University (TEAM ZJU-China)
cI Promoter Screen
Drew Endy - Stanford
Thomas Schneider - NIH
Want to sponsor us? Send an email to mtfacciotti@ucdavis.edu to discuss various ways you can help! :)
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