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Team:Tsinghua/Notebook/31 August 2010
From 2010.igem.org
(Difference between revisions)
(New page: == YX's part == PCR amplify the resistance genes. (First round) Then ligate the PCR products into T vector (pEASY T1, TransGen) After transformation, the trans1-T1 cells were spread onto...) |
(→Module I, group 2c) |
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== YX's part == | == YX's part == | ||
| - | + | Picked three clones of each resistance gene and shook in 3ml LB media. | |
| - | + | Extracted the plasmid and did PCR to verify the sequence. | |
| - | + | The positive clones were sent for sequencing. | |
| + | |||
| + | == Module I, group 2c == | ||
| + | |||
| + | Digest plasmid PSB1C13 with XbaI and SpeI, AP is used to dephosphorylate the 5' end of digestion product. | ||
| + | ---- | ||
| + | digestion system---- | ||
| + | |||
| + | PSB1C13 15ul | ||
| + | 10X M buffer 2ul | ||
| + | XbaI 1.5ul | ||
| + | SpeI 1.5ul | ||
| + | ---- | ||
| + | Incubate at 37°C overnight. | ||
Latest revision as of 20:57, 26 October 2010
YX's part
Picked three clones of each resistance gene and shook in 3ml LB media.
Extracted the plasmid and did PCR to verify the sequence.
The positive clones were sent for sequencing.
Module I, group 2c
Digest plasmid PSB1C13 with XbaI and SpeI, AP is used to dephosphorylate the 5' end of digestion product.
digestion system----
PSB1C13 15ul 10X M buffer 2ul XbaI 1.5ul SpeI 1.5ul
Incubate at 37°C overnight.
