Team:UNIPV-Pavia/Freezer
From 2010.igem.org
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Line 3: | Line 3: | ||
<td colspan="2">{{UNIPV-Pavia/header}}</td> | <td colspan="2">{{UNIPV-Pavia/header}}</td> | ||
</tr> | </tr> | ||
- | <tr><td | + | <tr> |
- | + | <td colspan="2">{{UNIPV-Pavia/menuAlto}}</td> | |
+ | </tr> | ||
+ | <tr><td valign=top> | ||
<!-- Contenuti --> | <!-- Contenuti --> | ||
{{UNIPV-Pavia/Style}} | {{UNIPV-Pavia/Style}} | ||
<table border="0" align="center" width="100%"><tr><td align="justify" valign="top" style="padding:20px"> | <table border="0" align="center" width="100%"><tr><td align="justify" valign="top" style="padding:20px"> | ||
<html><p align="center"><font size="4"><b>FREEZER MANAGEMENT</b></font></p></html><hr><br> | <html><p align="center"><font size="4"><b>FREEZER MANAGEMENT</b></font></p></html><hr><br> | ||
+ | <div align="center"> | ||
+ | For a detailed correspondence between our BioBrick "wiki names" and submitted BioBrick codes take a look [https://2010.igem.org/Team:UNIPV-Pavia/Calendar/October/settimana3#October.2C_18th here]. | ||
+ | </div> | ||
<table class="cont" border=0> | <table class="cont" border=0> | ||
- | |||
<tr> | <tr> | ||
<th>Biobrick name</th> | <th>Biobrick name</th> | ||
Line 21: | Line 25: | ||
<th>Store</th> | <th>Store</th> | ||
</tr> | </tr> | ||
- | |||
<!-- Parti e BB richiesti per l'iGEM - BioBrick 2010--> | <!-- Parti e BB richiesti per l'iGEM - BioBrick 2010--> | ||
- | |||
- | |||
<tr> | <tr> | ||
<td> | <td> | ||
Line 299: | Line 300: | ||
<td>937 bp</td> | <td>937 bp</td> | ||
<td>BioBricks 2010</td> | <td>BioBricks 2010</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">pSB1C3_RFP</font></td> | ||
+ | <td> | ||
+ | <partinfo>BBa_J04450</partinfo> <br/> in <partinfo>pSB1C3</partinfo>. | ||
+ | </td> | ||
+ | <td> | ||
+ | ?? | ||
+ | </td><td> | ||
+ | ''E. coli'' DH5alpha | ||
+ | </td><td> | ||
+ | <partinfo>pSB1C3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | |||
+ | </td> | ||
+ | <td> | ||
+ | 1069 bp | ||
+ | </td> | ||
+ | <td>2009 Distribution</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">pSB1C3_ccdB</font></td> | ||
+ | <td> | ||
+ | <partinfo>BBa_P1010</partinfo> <br/> in <partinfo>pSB1C3</partinfo>. | ||
+ | </td> | ||
+ | <td> | ||
+ | ?? | ||
+ | </td><td> | ||
+ | ''E. coli'' DH5alpha | ||
+ | </td><td> | ||
+ | <partinfo>pSB1C3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | |||
+ | </td> | ||
+ | <td> | ||
+ | 675 bp | ||
+ | </td> | ||
+ | <td>2009 Distribution</td> | ||
</tr> | </tr> | ||
Line 306: | Line 352: | ||
<!--Ligazioni fatte durante l'iGEM2010 --> | <!--Ligazioni fatte durante l'iGEM2010 --> | ||
<tr> | <tr> | ||
- | <th> | + | <th>Name</th> |
- | <th> | + | <th>Ligation</th> |
<th>Creation date</th> | <th>Creation date</th> | ||
<th>Strain</th> | <th>Strain</th> | ||
Line 313: | Line 359: | ||
<th>Quality control</th> | <th>Quality control</th> | ||
<th>Length</th> | <th>Length</th> | ||
- | <th> | + | <th>Description</th> |
</tr> | </tr> | ||
Line 321: | Line 367: | ||
<font class="BBok">I0</font> | <font class="BBok">I0</font> | ||
<br/>(it was I0-2) | <br/>(it was I0-2) | ||
+ | <br/><partinfo>BBa_k300005</partinfo> | ||
</td><td> | </td><td> | ||
<partinfo>BBa_K125500</partinfo> (E-S)<br>+<br><partinfo>BBa_B0015</partinfo> (E-X) | <partinfo>BBa_K125500</partinfo> (E-S)<br>+<br><partinfo>BBa_B0015</partinfo> (E-X) | ||
Line 332: | Line 379: | ||
Seq ok (BMR); length ok. | Seq ok (BMR); length ok. | ||
</td> | </td> | ||
- | <td> | + | <td>854 bp</td> |
+ | <td>GFPs + TT</td> | ||
</tr> | </tr> | ||
Line 341: | Line 389: | ||
<br/> | <br/> | ||
(it was I1-2) | (it was I1-2) | ||
+ | <br/><partinfo>BBa_k300006</partinfo> | ||
</td><td> | </td><td> | ||
<partinfo>BBa_E2050</partinfo> (E-S)<br>+<br><partinfo>BBa_K165018</partinfo> (E-X)</td><td> | <partinfo>BBa_E2050</partinfo> (E-S)<br>+<br><partinfo>BBa_K165018</partinfo> (E-X)</td><td> | ||
Line 352: | Line 401: | ||
</td> | </td> | ||
<td>1029 bp</td> | <td>1029 bp</td> | ||
- | <td> | + | <td>mOrange + NSL-ADH1 terminator</td> |
</tr> | </tr> | ||
Line 373: | Line 422: | ||
Seq ok (BMR); Cm resistance ok; length ok. | Seq ok (BMR); Cm resistance ok; length ok. | ||
</td> | </td> | ||
- | <td>906 bp</td><td> | + | <td>906 bp</td> |
+ | <td>Cm resistance + TT</td> | ||
</tr> | </tr> | ||
Line 383: | Line 433: | ||
<br/> | <br/> | ||
(it was I3-1) | (it was I3-1) | ||
+ | <br/><partinfo>BBa_k300009</partinfo> | ||
</td><td> | </td><td> | ||
<partinfo>BBa_K081008</partinfo> (E-S)<br>+<br><partinfo>BBa_B0015</partinfo> (E-X)</td> | <partinfo>BBa_K081008</partinfo> (E-S)<br>+<br><partinfo>BBa_B0015</partinfo> (E-X)</td> | ||
Line 394: | Line 445: | ||
Seq TWO PLASMIDS IN THIS CLONE!(BMR), two overlapped traces; length ok. | Seq TWO PLASMIDS IN THIS CLONE!(BMR), two overlapped traces; length ok. | ||
</td> | </td> | ||
- | <td>801 bp</td><td> | + | <td>801 bp</td> |
+ | <td>RBS + luxI + TT</td> | ||
</tr> | </tr> | ||
Line 415: | Line 467: | ||
</td> | </td> | ||
<td>1439 bp</td> | <td>1439 bp</td> | ||
- | <td> | + | <td>pTEF + mOrange + NLS + terminator</td> |
</tr> | </tr> | ||
Line 423: | Line 475: | ||
<br/> | <br/> | ||
(it was I5-1) | (it was I5-1) | ||
+ | <br/><partinfo>BBa_k300008</partinfo> | ||
</td><td> | </td><td> | ||
I2 (E-S)<br>+<br><partinfo>BBa_J61001</partinfo> (E-X)</td> | I2 (E-S)<br>+<br><partinfo>BBa_J61001</partinfo> (E-X)</td> | ||
Line 434: | Line 487: | ||
Seq ok (BMR); Cm resistance ok; length ok. | Seq ok (BMR); Cm resistance ok; length ok. | ||
</td> | </td> | ||
- | <td>1320 bp</td><td> | + | <td>1320 bp</td> |
+ | <td>Cm resistance + TT + R6K ori</td> | ||
</tr> | </tr> | ||
Line 454: | Line 508: | ||
</td> | </td> | ||
<td>2754 bp</td> | <td>2754 bp</td> | ||
- | <td> | + | <td>RBS + luxI + TT + HSL sensor</td> |
</tr> | </tr> | ||
Line 462: | Line 516: | ||
<font class="BBok">I7</font> | <font class="BBok">I7</font> | ||
<br/> | <br/> | ||
- | (it was I7-3)<br/> | + | (it was I7-3) |
+ | <br/><partinfo>BBa_k300024</partinfo> | ||
</td><td> | </td><td> | ||
- | <partinfo>BBa_J23118</partinfo> ( | + | <partinfo>BBa_J23118</partinfo> (S-P)<br/>+<br/>I6 (X-P)</td> |
<td> | <td> | ||
25/06/2010 | 25/06/2010 | ||
Line 476: | Line 531: | ||
</td> | </td> | ||
<td>2795 bp</td> | <td>2795 bp</td> | ||
- | <td> | + | <td>constitutive promoter + RBS + luxI + TT + HSL sensor</td> |
</tr> | </tr> | ||
Line 485: | Line 540: | ||
<br/> | <br/> | ||
(it was I8-5) | (it was I8-5) | ||
+ | <br/><partinfo>BBa_K300021</partinfo> | ||
</td><td> | </td><td> | ||
- | <partinfo>BBa_J23110</partinfo> ( | + | <partinfo>BBa_J23110</partinfo> (S-P)<br/>+<br/>I6 (X-P)</td> |
<td> | <td> | ||
25/06/2010 | 25/06/2010 | ||
Line 498: | Line 554: | ||
</td> | </td> | ||
<td>2795 bp</td> | <td>2795 bp</td> | ||
- | <td> | + | <td>constitutive promoter + RBS + luxI + TT + HSL sensor</td> |
</tr> | </tr> | ||
Line 506: | Line 562: | ||
<br/> | <br/> | ||
(it was I9-1) | (it was I9-1) | ||
+ | <br/><partinfo>BBa_K300022</partinfo> | ||
</td><td> | </td><td> | ||
- | <partinfo> | + | <partinfo>BBa_J23114</partinfo> (S-P)<br/>+<br/>I6 (X-P)</td> |
<td> | <td> | ||
25/06/2010 | 25/06/2010 | ||
Line 519: | Line 576: | ||
</td> | </td> | ||
<td>2795 bp</td> | <td>2795 bp</td> | ||
- | <td> | + | <td>constitutive promoter + RBS + luxI + TT + HSL sensor</td> |
</tr> | </tr> | ||
Line 528: | Line 585: | ||
<br/> | <br/> | ||
(it was I10-1) | (it was I10-1) | ||
+ | <br/><partinfo>BBa_K300023</partinfo> | ||
</td><td> | </td><td> | ||
- | <partinfo>BBa_J23116</partinfo> ( | + | <partinfo>BBa_J23116</partinfo> (S-P)<br/>+<br/>I6 (X-P)</td> |
<td> | <td> | ||
25/06/2010 | 25/06/2010 | ||
Line 541: | Line 599: | ||
</td> | </td> | ||
<td>2795 bp</td> | <td>2795 bp</td> | ||
- | <td> | + | <td>constitutive promoter + RBS + luxI + TT + HSL sensor</td> |
</tr> | </tr> | ||
Line 550: | Line 608: | ||
<br/> | <br/> | ||
(it was I12-2) | (it was I12-2) | ||
+ | <br/><partinfo>BBa_k300019</partinfo> | ||
</td> | </td> | ||
<td> | <td> | ||
Line 565: | Line 624: | ||
</td> | </td> | ||
<td>2795 bp</td> | <td>2795 bp</td> | ||
- | <td> | + | <td>constitutive promoter + RBS + luxI + TT + HSL sensor</td> |
</tr> | </tr> | ||
Line 574: | Line 633: | ||
<br/> | <br/> | ||
(it was I14-1) | (it was I14-1) | ||
+ | <br/><partinfo>BBa_k300030</partinfo> | ||
</td> | </td> | ||
<td> | <td> | ||
- | <partinfo> | + | <partinfo>BBa_J23118</partinfo> (S-P)<br/>+<br/>I3 (X-P) |
</td> | </td> | ||
<td> | <td> | ||
Line 589: | Line 649: | ||
</td> | </td> | ||
<td>842 bp</td> | <td>842 bp</td> | ||
- | <td> | + | <td>constitutive promoter + RBS + luxI + TT</td> |
</tr> | </tr> | ||
Line 615: | Line 675: | ||
1328 bp | 1328 bp | ||
</td> | </td> | ||
- | <td> | + | <td>Cm resistance + TT + R6K ori</td> |
</tr> | </tr> | ||
Line 623: | Line 683: | ||
<br/> | <br/> | ||
(it was I15-1) | (it was I15-1) | ||
+ | <br/><partinfo>BBa_k300028</partinfo> | ||
</td> | </td> | ||
<td> | <td> | ||
Line 638: | Line 699: | ||
</td> | </td> | ||
<td>842 bp</td> | <td>842 bp</td> | ||
- | <td> | + | <td>constitutive promoter + RBS + luxI + TT</td> |
</tr> | </tr> | ||
Line 647: | Line 708: | ||
<br/> | <br/> | ||
(it was I16-1) | (it was I16-1) | ||
+ | <br/><partinfo>BBa_k300029</partinfo> | ||
</td> | </td> | ||
<td> | <td> | ||
Line 662: | Line 724: | ||
</td> | </td> | ||
<td>842 bp</td> | <td>842 bp</td> | ||
- | <td> | + | <td>constitutive promoter + RBS + luxI + TT</td> |
</tr> | </tr> | ||
Line 671: | Line 733: | ||
<br/> | <br/> | ||
(it was I17-1) | (it was I17-1) | ||
+ | <br/><partinfo>BBa_k300025</partinfo> | ||
</td> | </td> | ||
<td> | <td> | ||
Line 685: | Line 748: | ||
</td> | </td> | ||
<td>842 bp</td> | <td>842 bp</td> | ||
- | <td> | + | <td>constitutive promoter + RBS + luxI + TT</td> |
</tr> | </tr> | ||
Line 694: | Line 757: | ||
<br/> | <br/> | ||
(it was I18-1) | (it was I18-1) | ||
+ | <br/><partinfo>BBa_k300026</partinfo> | ||
</td> | </td> | ||
<td> | <td> | ||
Line 709: | Line 773: | ||
</td> | </td> | ||
<td>842 bp</td> | <td>842 bp</td> | ||
- | <td> | + | <td>constitutive promoter + RBS + luxI + TT</td> |
</tr> | </tr> | ||
Line 718: | Line 782: | ||
<br/> | <br/> | ||
(it was I19-1) | (it was I19-1) | ||
+ | <br/><partinfo>BBa_k300027</partinfo> | ||
</td> | </td> | ||
<td> | <td> | ||
Line 733: | Line 798: | ||
</td> | </td> | ||
<td>842 bp</td> | <td>842 bp</td> | ||
- | <td> | + | <td>constitutive promoter + RBS + luxI + TT</td> |
</tr> | </tr> | ||
Line 742: | Line 807: | ||
<br/> | <br/> | ||
(it was I20M-1) | (it was I20M-1) | ||
+ | <br/><partinfo>BBa_k300002</partinfo> | ||
</td> | </td> | ||
<td> | <td> | ||
- | + | Pha10S-1 (X-S) (infos <html><a href="https://2010.igem.org/Team:UNIPV-Pavia/Calendar/July/settimana5#July.2C_29th" target="_blank">here</a></html>) <br/>+<br/> <partinfo>pSB1AK3</partinfo> (X-S) | |
</td> | </td> | ||
<td> | <td> | ||
Line 756: | Line 822: | ||
</td> | </td> | ||
<td> | <td> | ||
- | + | Seq ok (BMR), length ok. | |
</td> | </td> | ||
<td> | <td> | ||
576 bp | 576 bp | ||
</td> | </td> | ||
- | <td> | + | <td>Pha10S</td> |
</tr> | </tr> | ||
Line 770: | Line 836: | ||
<br/> | <br/> | ||
(it was I21-4) | (it was I21-4) | ||
+ | <br/><partinfo>BBa_k300003</partinfo> | ||
</td> | </td> | ||
<td> | <td> | ||
- | + | PhaSS-1 (X-S) (infos <html><a href="https://2010.igem.org/Team:UNIPV-Pavia/Calendar/August/settimana2#August.2C_10th" target="_blank">here</a></html>) <br/>+<br/> <partinfo>pSB1A3</partinfo> (X-S) | |
</td> | </td> | ||
<td> | <td> | ||
Line 781: | Line 848: | ||
<partinfo>pSB1A3</partinfo> | <partinfo>pSB1A3</partinfo> | ||
</td><td> | </td><td> | ||
- | + | Seq ok (BMR), length ok. | |
</td> | </td> | ||
<td> | <td> | ||
576 bp | 576 bp | ||
</td> | </td> | ||
- | <td> | + | <td>PhaSS</td> |
</tr> | </tr> | ||
Line 795: | Line 862: | ||
<br/> | <br/> | ||
(it was I22-1) | (it was I22-1) | ||
+ | <br/><partinfo>BBa_k300031</partinfo> | ||
</td> | </td> | ||
<td> | <td> | ||
Line 811: | Line 879: | ||
825 bp | 825 bp | ||
</td> | </td> | ||
- | <td> | + | <td>RBS + lasI + terminator</td> |
</tr> | </tr> | ||
Line 836: | Line 904: | ||
725 bp | 725 bp | ||
</td> | </td> | ||
- | <td> | + | <td>plux + RBS + luxI + TT</td> |
</tr> | </tr> | ||
Line 863: | Line 931: | ||
886 bp | 886 bp | ||
</td> | </td> | ||
- | <td> | + | <td>plux + RBS + lasI + terminator</td> |
</tr> | </tr> | ||
Line 872: | Line 940: | ||
<br/> | <br/> | ||
(it was I26-2) | (it was I26-2) | ||
+ | <br/><partinfo>BBa_k300081</partinfo> | ||
</td> | </td> | ||
<td> | <td> | ||
- | <partinfo>BBa_K105012</partinfo> (S-P) <br/>+<br/> I21 | + | <partinfo>BBa_K105012</partinfo> (S-P) <br/>+<br/> I21 (X-P) |
</td> | </td> | ||
<td> | <td> | ||
Line 885: | Line 954: | ||
</td> | </td> | ||
<td> | <td> | ||
- | + | Seq ok (BMR), length ok. | |
</td> | </td> | ||
<td> | <td> | ||
612 bp | 612 bp | ||
</td> | </td> | ||
- | <td> | + | <td>linker + PhaSS</td> |
</tr> | </tr> | ||
Line 918: | Line 987: | ||
2678 bp | 2678 bp | ||
</td> | </td> | ||
- | <td> | + | <td>HLS sensor + plux + RBS + luxI + TT</td> |
</tr> | </tr> | ||
Line 925: | Line 994: | ||
<td> | <td> | ||
I30 | I30 | ||
- | |||
- | |||
</td> | </td> | ||
<td> | <td> | ||
Line 946: | Line 1,013: | ||
2839 bp | 2839 bp | ||
</td> | </td> | ||
- | <td> | + | <td>HSL sensor + plux + RBS + lasI + terminator</td> |
</tr> | </tr> | ||
Line 955: | Line 1,022: | ||
<br/> | <br/> | ||
(it was I31-1) | (it was I31-1) | ||
+ | <br/><partinfo>BBa_K300079</partinfo> | ||
</td> | </td> | ||
<td> | <td> | ||
Line 969: | Line 1,037: | ||
</td> | </td> | ||
<td> | <td> | ||
- | + | Seq ok (BMR), length ok. | |
</td> | </td> | ||
<td> | <td> | ||
- | + | 612 bp | |
</td> | </td> | ||
- | <td> | + | <td>Pha10S + linker</td> |
</tr> | </tr> | ||
Line 996: | Line 1,064: | ||
</td> | </td> | ||
<td> | <td> | ||
- | + | Seq ok (BMR), length ok. | |
</td> | </td> | ||
<td> | <td> | ||
1158 bp | 1158 bp | ||
</td> | </td> | ||
- | <td> | + | <td>Pha10S + PhaSS</td> |
</tr> | </tr> | ||
Line 1,022: | Line 1,090: | ||
</td> | </td> | ||
<td> | <td> | ||
- | + | Seq ok (BMR), length ok. | |
</td> | </td> | ||
<td> | <td> | ||
- | + | 1436 bp | |
</td> | </td> | ||
- | <td> | + | <td>Pha10S + GFPs + TT</td> |
</tr> | </tr> | ||
Line 1,049: | Line 1,117: | ||
</td> | </td> | ||
<td> | <td> | ||
- | + | Seq ok (BMR), length ok. | |
</td> | </td> | ||
<td> | <td> | ||
- | + | 1436 bp | |
</td> | </td> | ||
- | <td> | + | <td>PhaSS + GFPs + TT</td> |
</tr> | </tr> | ||
Line 1,063: | Line 1,131: | ||
<br/> | <br/> | ||
(it was I35-1) | (it was I35-1) | ||
+ | <br/><partinfo>BBa_K300080</partinfo> | ||
</td> | </td> | ||
<td> | <td> | ||
Line 1,076: | Line 1,145: | ||
</td> | </td> | ||
<td> | <td> | ||
- | + | Seq ok (BMR), length ok. | |
</td> | </td> | ||
<td> | <td> | ||
- | + | 612 bp | |
</td> | </td> | ||
- | <td> | + | <td>PhaSS + linker</td> |
</tr> | </tr> | ||
Line 1,108: | Line 1,177: | ||
1158 bp | 1158 bp | ||
</td> | </td> | ||
- | <td> | + | <td>PhaSS + PhaSS</td> |
</tr> | </tr> | ||
Line 1,130: | Line 1,199: | ||
</td> | </td> | ||
<td> | <td> | ||
- | + | Seq ok (BMR), length ok. | |
</td> | </td> | ||
<td> | <td> | ||
- | + | 1367 bp | |
</td> | </td> | ||
- | <td> | + | <td>INTEIN + GFPs + TT</td> |
</tr> | </tr> | ||
Line 1,157: | Line 1,226: | ||
</td> | </td> | ||
<td> | <td> | ||
- | + | Seq ok (BMR), length ok. | |
</td> | </td> | ||
<td> | <td> | ||
- | + | 1081 bp | |
</td> | </td> | ||
- | <td> | + | <td>HSL inducible system</td> |
</tr> | </tr> | ||
Line 1,183: | Line 1,252: | ||
</td> | </td> | ||
<td> | <td> | ||
- | + | Seq ok (BMR), length ok. | |
</td> | </td> | ||
<td> | <td> | ||
- | + | 656 bp | |
</td> | </td> | ||
- | <td> | + | <td>pTet + Pha10S</td> |
</tr> | </tr> | ||
Line 1,215: | Line 1,284: | ||
1125 bp | 1125 bp | ||
</td> | </td> | ||
- | <td> | + | <td>linker + PhaSS + INTEIN</td> |
</tr> | </tr> | ||
Line 1,237: | Line 1,306: | ||
</td> | </td> | ||
<td> | <td> | ||
- | + | Seq ok (BMR), length ok. | |
</td> | </td> | ||
<td> | <td> | ||
- | + | 1472 bp | |
</td> | </td> | ||
- | <td> | + | <td>linker + PhaSS + GFPs + TT</td> |
</tr> | </tr> | ||
Line 1,255: | Line 1,324: | ||
</td> | </td> | ||
<td> | <td> | ||
- | + | 23/08/2010 | |
</td><td> | </td><td> | ||
''E. coli'' BW23474 | ''E. coli'' BW23474 | ||
Line 1,268: | Line 1,337: | ||
3068 bp | 3068 bp | ||
</td> | </td> | ||
- | <td> | + | <td>CREAM fragment + Cm resistance + TT + R6K ori + constitutive GFP</td> |
</tr> | </tr> | ||
Line 1,281: | Line 1,350: | ||
</td> | </td> | ||
<td> | <td> | ||
- | + | 23/08/2010 | |
</td><td> | </td><td> | ||
''E. coli'' BW23474 | ''E. coli'' BW23474 | ||
Line 1,294: | Line 1,363: | ||
3026 bp | 3026 bp | ||
</td> | </td> | ||
- | <td> | + | <td>CREAM fragment + Cm resistance + TT + R6K ori + constitutive promoter</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td> | <td> | ||
- | I44<br/> | + | <font class="BBok">I44</font> |
+ | <br/> | ||
+ | (it was I44-2) | ||
<br/> | <br/> | ||
</td> | </td> | ||
Line 1,306: | Line 1,377: | ||
</td> | </td> | ||
<td> | <td> | ||
- | + | 15/09/2010 | |
- | + | ||
</td><td> | </td><td> | ||
''E. coli'' DB3.1 | ''E. coli'' DB3.1 | ||
Line 1,315: | Line 1,385: | ||
</td> | </td> | ||
<td> | <td> | ||
- | + | Seq ok (BMR). | |
</td> | </td> | ||
<td> | <td> | ||
3239 bp | 3239 bp | ||
</td> | </td> | ||
- | <td> | + | <td>CREAM fragment + Cm resistance + TT + R6K ori + marker ccdB</td> |
</tr> | </tr> | ||
Line 1,334: | Line 1,404: | ||
</td> | </td> | ||
<td> | <td> | ||
- | + | 23/08/2010 | |
</td><td> | </td><td> | ||
''E. coli'' BW23474 | ''E. coli'' BW23474 | ||
Line 1,347: | Line 1,417: | ||
2149 bp | 2149 bp | ||
</td> | </td> | ||
- | <td> | + | <td>CREAM fragment + Cm resistance + TT + R6K ori</td> |
</tr> | </tr> | ||
Line 1,356: | Line 1,426: | ||
<br/> | <br/> | ||
(it was I47-13) | (it was I47-13) | ||
+ | <br/><partinfo>BBa_K300086</partinfo> | ||
<br/> | <br/> | ||
</td> | </td> | ||
Line 1,364: | Line 1,435: | ||
02/09/2010 | 02/09/2010 | ||
</td><td> | </td><td> | ||
- | ''E. coli'' | + | ''E. coli'' TOP10 |
</td> | </td> | ||
<td> | <td> | ||
Line 1,370: | Line 1,441: | ||
</td> | </td> | ||
<td> | <td> | ||
- | + | Seq ok (BMR), length ok. | |
</td> | </td> | ||
<td> | <td> | ||
- | + | 1516 bp | |
</td> | </td> | ||
- | <td> | + | <td>pTet + Pha10S + GFPs + TT</td> |
</tr> | </tr> | ||
Line 1,384: | Line 1,455: | ||
<br/> | <br/> | ||
(it was I48-1) | (it was I48-1) | ||
+ | <br/><partinfo>BBa_K300088</partinfo> | ||
<br/> | <br/> | ||
</td> | </td> | ||
Line 1,392: | Line 1,464: | ||
31/08/2010 | 31/08/2010 | ||
</td><td> | </td><td> | ||
- | ''E. coli'' | + | ''E. coli'' TOP10 |
</td> | </td> | ||
<td> | <td> | ||
Line 1,398: | Line 1,470: | ||
</td> | </td> | ||
<td> | <td> | ||
- | + | Seq ok (BMR), length ok. | |
</td> | </td> | ||
<td> | <td> | ||
- | + | 2098 bp | |
</td> | </td> | ||
- | <td> | + | <td>pTet + Pha10S + PhaSS + GFPs + TT</td> |
</tr> | </tr> | ||
Line 1,411: | Line 1,483: | ||
<br/> | <br/> | ||
(it was I49-1) | (it was I49-1) | ||
+ | <br/><partinfo>BBa_K300090</partinfo> | ||
<br/> | <br/> | ||
</td> | </td> | ||
Line 1,419: | Line 1,492: | ||
31/08/2010 | 31/08/2010 | ||
</td><td> | </td><td> | ||
- | ''E. coli'' | + | ''E. coli'' TOP10 |
</td> | </td> | ||
<td> | <td> | ||
Line 1,425: | Line 1,498: | ||
</td> | </td> | ||
<td> | <td> | ||
- | + | Seq ok (BMR), length ok. | |
</td> | </td> | ||
<td> | <td> | ||
- | + | 2134 bp | |
</td> | </td> | ||
- | <td> | + | <td>pTet + Pha10S + linker + PhaSS + GFPs + TT</td> |
</tr> | </tr> | ||
Line 1,437: | Line 1,510: | ||
<td> | <td> | ||
I50<br/> | I50<br/> | ||
+ | FAILED (no trasformants on plate) | ||
<br/> | <br/> | ||
</td> | </td> | ||
<td> | <td> | ||
I44 (E-H) on itself | I44 (E-H) on itself | ||
+ | </td> | ||
+ | <td> | ||
+ | 28/08/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' DB3.1 | ||
+ | </td> | ||
+ | <td> | ||
+ | CREAM | ||
</td> | </td> | ||
<td> | <td> | ||
+ | </td> | ||
+ | <td> | ||
+ | 3239 bp | ||
+ | </td> | ||
+ | <td>CREAM fragment + Cm resistance + TT + R6K ori + marker ccdB</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I51</font> | ||
+ | <br/> | ||
+ | (it was I51-3) | ||
+ | <br/><partinfo>BBa_I52002</partinfo> | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | CREAM (E-P) <br/>+<br/><partinfo>BBa_I52002</partinfo> (E-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 03/09/2010 | ||
</td><td> | </td><td> | ||
''E. coli'' DB3.1 | ''E. coli'' DB3.1 | ||
Line 1,457: | Line 1,560: | ||
3239 bp | 3239 bp | ||
</td> | </td> | ||
- | <td> | + | <td>CREAM fragment + Cm resistance + TT + R6K ori + marker ccdB</td> |
</tr> | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I52</font> | ||
+ | <br/> | ||
+ | (it was I52-c) | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I35 (S-P)<br/>+<br/>I37 (X-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 17/09/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' DH5-alpha | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1A2</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 1985 bp | ||
+ | </td> | ||
+ | <td>PhaSS + linker + INTEIN + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I53</font> | ||
+ | <br/> | ||
+ | (it was I53-c) | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I32 (E-S)<br/>+<br/>I37 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 17/09/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' DH5-alpha | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1AK3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 2531 bp | ||
+ | </td> | ||
+ | <td>Pha10S + PhaSS + INTEIN + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I54</font> | ||
+ | <br/> | ||
+ | (it was I54-a) | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I36 (E-S)<br/>+<br/>I37 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 17/09/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' DH5-alpha | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1AK3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 2531 bp | ||
+ | </td> | ||
+ | <td>PhaSS + PhaSS + INTEIN + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I55</font> | ||
+ | <br/> | ||
+ | (it was I55-1) | ||
+ | <br/><partinfo>BBa_K300082</partinfo> | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I38 (E-S)<br/>+<br/>I20 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 02/10/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' DH5-alpha | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1AK3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 1663 bp | ||
+ | </td> | ||
+ | <td>HSL inducible system + Pha10S</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I56</font> | ||
+ | <br/> | ||
+ | (it was I56-a) | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I26 (E-S)<br/>+<br/>I41 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 17/09/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' DH5-alpha | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1AK3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 2090 bp | ||
+ | </td> | ||
+ | <td>linker + PhaSS + linker + PhaSS + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I57</font> | ||
+ | <br/> | ||
+ | (it was I57-a) | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I40 (E-S)<br/>+<br/>I0 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 17/09/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' DH5-alpha | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1AK3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 1985 bp | ||
+ | </td> | ||
+ | <td>linker + PhaSS + INTEIN + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I58</font> | ||
+ | <br/> | ||
+ | (it was I58-c) | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I38 (E-S)<br/>+<br/>I31 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 17/09/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' DH5-alpha | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1A2</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 1699 bp | ||
+ | </td> | ||
+ | <td>HSL inducible system + Pha10S + linker</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I59</font> | ||
+ | <br/> | ||
+ | (it was I59-b) | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I21 (E-S)<br/> + <br/>I34 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 17/09/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' DH5-alpha | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1AK3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 2018 bp | ||
+ | </td> | ||
+ | <td>PhaSS + PhaSS + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I60</font> | ||
+ | <br/> | ||
+ | (it was I60-a) | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I20 (S-P)<br/>+<br/>I26 (X-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 17/09/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' DH5-alpha | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1AK3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 1194 bp | ||
+ | </td> | ||
+ | <td>Pha10S + linker + PhaSS</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I61</font> | ||
+ | <br/> | ||
+ | (it was I61-a) | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I31 (E-S)<br/>+<br/>I37 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 17/09/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' DH5-alpha | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1AK3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 1985 bp | ||
+ | </td> | ||
+ | <td>Pha10S + linker + INTEIN + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I62</font> | ||
+ | <br/> | ||
+ | (it was I62-E) | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I39 (S-P)<br/>+<br/>I59 (X-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 27/09/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1A2</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 2678 bp | ||
+ | </td> | ||
+ | <td>pTet + Pha10S + PhaSS + PhaSS + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I63</font> | ||
+ | <br/> | ||
+ | (it was I63-1) | ||
+ | <br/><partinfo>BBa_K300091</partinfo> | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I38 (E-S)<br/>+<br/>I61 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 24/09/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1AK3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 3072 bp | ||
+ | </td> | ||
+ | <td>HSL inducible system + Pha10S + linker + INTEIN + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I64</font> | ||
+ | <br/> | ||
+ | (it was I64-2) | ||
+ | <br/><partinfo>BBa_K300092</partinfo> | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I58 (E-S)<br/>+<br/>I52 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 24/09/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1A2</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 3690 bp | ||
+ | </td> | ||
+ | <td>HSL inducible system + Pha10S + linker + PhaSS + linker + INTEIN + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I65</font> | ||
+ | <br/> | ||
+ | (it was I65-3) | ||
+ | <br/><partinfo>BBa_K300099</partinfo> | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I39 (S-P)<br/>+<br/>I56 (X-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 24/09/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1A2</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 2752 bp | ||
+ | </td> | ||
+ | <td>pTet + Pha10S + linker + PhaSS + linker + PhaSS + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I66</font> | ||
+ | <br/> | ||
+ | (it was I66-1) | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I60 (E-S)<br/>+<br/>I57 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 24/09/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1AK3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 3185 bp | ||
+ | </td> | ||
+ | <td>Pha10S + linker + PhaSS + linker + PhaSS + INTEIN + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I70</font> | ||
+ | <br/> | ||
+ | (it was I70-1) | ||
+ | <br/><partinfo>BBa_K300007</partinfo> | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | MyYeast3 (E-S)<br/>+<br/>I4 (E-S) | ||
+ | </td> | ||
+ | <td> | ||
+ | 01/10/10 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | MyYeast (Amp100 in coli) | ||
+ | </td> | ||
+ | <td> | ||
+ | |||
+ | </td> | ||
+ | <td> | ||
+ | 5187bp | ||
+ | </td> | ||
+ | <td>MyYeast vector + I4 in cloning site (Ptef1 + mOrange + Tadh1)</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I71</font> | ||
+ | <br/> | ||
+ | (it was I71-1) | ||
+ | </td> | ||
+ | <td> | ||
+ | MyYeast3 (E-S)<br/>+<br/>I1 (E-S) | ||
+ | </td> | ||
+ | <td> | ||
+ | 01/10/10 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | MyYeast (Amp100 in coli) | ||
+ | </td> | ||
+ | <td> | ||
+ | |||
+ | </td> | ||
+ | <td> | ||
+ | 4778bp | ||
+ | </td> | ||
+ | <td>MyYeast vector + I1 in cloning site (mOrange + Tadh1, NO promoter)</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | I72<br/> | ||
+ | (it was I70-x) | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>BBa_J04450</partinfo>(E-P)+<partinfo>pSB4C5</partinfo>(E-P)</td> | ||
+ | <td> | ||
+ | |||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB4C5</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | |||
+ | </td> | ||
+ | <td> | ||
+ | 4290bp | ||
+ | </td> | ||
+ | <td>mRFP under pLac promoter in LC plasmid pSB4C5</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I73</font> | ||
+ | <br/> | ||
+ | (it was I73-1) | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I38 (E-S)<br/>+<br/>I53 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 02/10/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1AK3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 3618 bp | ||
+ | </td> | ||
+ | <td>HSL inducible system + Pha10S + PhaSS + INTEIN + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I74</font> | ||
+ | <br/> | ||
+ | (it was I74-8) | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I31 (E-S)<br/>+<br/>I35 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 04/10/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1A2</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 1230 bp | ||
+ | </td> | ||
+ | <td>Pha10S + linker + PhaSS + linker</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | I75<br/> | ||
+ | (it was I75-x) | ||
+ | </td> | ||
+ | <td> | ||
+ | I55 (E-S)<br/>+<br/>I37 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | Not screened yet. | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1AK3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Not screened yet. | ||
+ | </td> | ||
+ | <td> | ||
+ | 3036 bp | ||
+ | </td> | ||
+ | <td>HSL inducible system + Pha10S + INTEIN + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | I76<br/> | ||
+ | (it was I76-x) | ||
+ | </td> | ||
+ | <td> | ||
+ | I55 (E-S)<br/>+<br/>I54 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | Not screened yet. | ||
+ | </td><td> | ||
+ | ''E. coli'' DH5-alpha | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1AK3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Not screened yet. | ||
+ | </td> | ||
+ | <td> | ||
+ | 4191 bp | ||
+ | </td> | ||
+ | <td>HSL inducible system + Pha10S + PhaSS + PhaSS + INTEIN + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | I77<br/> | ||
+ | (it was I77-x) | ||
+ | </td> | ||
+ | <td> | ||
+ | I55 (E-S)<br/>+<br/>I57 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | Not screened yet. | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1AK3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Not screened yet. | ||
+ | </td> | ||
+ | <td> | ||
+ | 3654 bp | ||
+ | </td> | ||
+ | <td>HSL inducible system + Pha10S + linker + PhaSS + INTEIN + GFPs + TT</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I78</font> | ||
+ | <br/> | ||
+ | (it was I78-3) | ||
+ | <br/><partinfo>BBa_K300084</partinfo> | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I74 (E-S)<br/>+<br/>INTEIN_C3 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 07/10/10 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1C3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 1743 bp | ||
+ | </td> | ||
+ | <td>Pha10S + linker + PhaSS + linker + INTEIN</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I79</font> | ||
+ | <br/> | ||
+ | (it was I79-2) | ||
+ | <br/><partinfo>BBa_K300083</partinfo> | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I60 (E-S)<br/>+<br/>INTEIN_C3 (E-X) | ||
+ | </td> | ||
+ | <td> | ||
+ | 07/10/10 | ||
+ | </td><td> | ||
+ | ''E. coli'' STBL3 | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1C3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 1707 bp | ||
+ | </td> | ||
+ | <td>Pha10S + linker + PhaSS + INTEIN</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">I80</font> | ||
+ | <br/> | ||
+ | (it was I80-4) | ||
+ | <br/><partinfo>BBa_K300010</partinfo> | ||
+ | <br/> | ||
+ | </td> | ||
+ | <td> | ||
+ | I3_1C3 (S-P)<br/>+<br/><partinfo>BBa_F2620</partinfo> (X-P) | ||
+ | </td> | ||
+ | <td> | ||
+ | 11/10/10 | ||
+ | </td><td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1C3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Seq ok (BMR), length ok. | ||
+ | </td> | ||
+ | <td> | ||
+ | 1707 bp | ||
+ | </td> | ||
+ | <td> | ||
+ | RBS + luxI + TT + <partinfo>BBa_F2620</partinfo> | ||
+ | </td> | ||
+ | </tr> | ||
Line 1,465: | Line 2,290: | ||
<td> | <td> | ||
<font class="BBok">INTEIN</font> | <font class="BBok">INTEIN</font> | ||
+ | <br/><partinfo>BBa_K300004</partinfo> | ||
</td> | </td> | ||
<td> | <td> | ||
Line 1,483: | Line 2,309: | ||
507 bp | 507 bp | ||
</td> | </td> | ||
- | <td> | + | <td>INTEIN</td> |
</tr> | </tr> | ||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">INTEIN_C3</font> | ||
+ | </td> | ||
+ | <td> | ||
+ | Intein | ||
+ | </td> | ||
+ | <td> | ||
+ | 02/10/2010 | ||
+ | </td> | ||
+ | <td> | ||
+ | ''E. coli'' TOP10 | ||
+ | </td> | ||
+ | <td> | ||
+ | <partinfo>pSB1C3</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | Mr.Gene DNA synthesis | ||
+ | </td> | ||
+ | <td> | ||
+ | 507 bp | ||
+ | </td> | ||
+ | <td> | ||
+ | INTEIN in <partinfo>pSB1C3</partinfo> | ||
+ | </td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | <tr> | ||
+ | <td> | ||
+ | <font class="BBok">YEAST<br/>MR.G</font> | ||
+ | <br/><partinfo>BBa_K300980</partinfo> | ||
+ | </td> | ||
+ | <td> | ||
+ | YEAST fragment | ||
+ | </td> | ||
+ | <td> | ||
+ | 15/09/2010 | ||
+ | </td><td> | ||
+ | ''E. coli'' DH5-alpha | ||
+ | </td> | ||
+ | <td> | ||
+ | pMA <br/> (HC Amp resistance) | ||
+ | </td> | ||
+ | <td> | ||
+ | Mr.Gene DNA synthesis | ||
+ | </td> | ||
+ | <td> | ||
+ | 1695 bp | ||
+ | </td> | ||
+ | <td>YEAST fragment</td> | ||
+ | </tr> | ||
+ | |||
<tr> | <tr> | ||
<td> | <td> | ||
<font class="BBok">CRIM<br/>MR.G</font> | <font class="BBok">CRIM<br/>MR.G</font> | ||
+ | <br/><partinfo>BBa_K300983</partinfo> | ||
</td> | </td> | ||
<td> | <td> | ||
Line 1,507: | Line 2,390: | ||
829 bp | 829 bp | ||
</td> | </td> | ||
- | <td> | + | <td>CREAM fragment</td> |
</tr> | </tr> | ||
Line 1,516: | Line 2,399: | ||
<br/> | <br/> | ||
(it was MyCRIM) | (it was MyCRIM) | ||
+ | <br/><partinfo>BBa_K300982</partinfo> | ||
<br/> | <br/> | ||
</td> | </td> | ||
Line 1,535: | Line 2,419: | ||
2149 bp | 2149 bp | ||
</td> | </td> | ||
- | <td> | + | <td>CREAM fragment + Cm resistance + TT + R6K ori</td> |
</tr> | </tr> | ||
Line 1,558: | Line 2,442: | ||
</td> | </td> | ||
<td>2795 bp</td> | <td>2795 bp</td> | ||
- | <td> | + | <td>constitutive promoter + RBS + luxI + TT + HSL sensor in <partinfo>pSB4C5</partinfo></td> |
</tr> | </tr> | ||
Line 1,581: | Line 2,465: | ||
</td> | </td> | ||
<td>2795 bp</td> | <td>2795 bp</td> | ||
- | <td> | + | <td>constitutive promoter + RBS + luxI + TT + HSL sensor in <partinfo>pSB4C5</partinfo></td> |
</tr> | </tr> | ||
Line 1,605: | Line 2,489: | ||
2795 bp | 2795 bp | ||
</td> | </td> | ||
- | <td> | + | <td>constitutive promoter + RBS + luxI + TT + HSL sensor in <partinfo>pSB4C5</partinfo></td> |
</tr> | </tr> | ||
Line 1,630: | Line 2,514: | ||
2795 bp | 2795 bp | ||
</td> | </td> | ||
- | <td> | + | <td>constitutive promoter + RBS + luxI + TT + HSL sensor in <partinfo>pSB4C5</partinfo></td> |
</tr> | </tr> | ||
Line 1,655: | Line 2,539: | ||
842 bp | 842 bp | ||
</td> | </td> | ||
- | <td> | + | <td>constitutive promoter + RBS + luxI + TT + HSL sensor in <partinfo>pSB4C5</partinfo></td> |
</tr> | </tr> | ||
Line 1,679: | Line 2,563: | ||
842 bp | 842 bp | ||
</td> | </td> | ||
- | <td> | + | <td>constitutive promoter + RBS + luxI + TT in <partinfo>pSB4C5</partinfo></td> |
</tr> | </tr> | ||
Line 1,704: | Line 2,588: | ||
842 bp | 842 bp | ||
</td> | </td> | ||
- | <td> | + | <td>constitutive promoter + RBS + luxI + TT in <partinfo>pSB4C5</partinfo></td> |
</tr> | </tr> | ||
Line 1,731: | Line 2,615: | ||
842 bp | 842 bp | ||
</td> | </td> | ||
- | <td> | + | <td>constitutive promoter + RBS + luxI + TT in <partinfo>pSB4C5</partinfo></td> |
</tr> | </tr> | ||
Line 1,756: | Line 2,640: | ||
842 bp | 842 bp | ||
</td> | </td> | ||
- | <td> | + | <td>constitutive promoter + RBS + luxI + TT in <partinfo>pSB4C5</partinfo></td> |
</tr> | </tr> | ||
Line 1,781: | Line 2,665: | ||
842 bp | 842 bp | ||
</td> | </td> | ||
- | <td> | + | <td>constitutive promoter + RBS + luxI + TT in <partinfo>pSB4C5</partinfo></td> |
</tr> | </tr> | ||
Line 1,790: | Line 2,674: | ||
I27 | I27 | ||
<br/> | <br/> | ||
- | |||
</td> | </td> | ||
<td> | <td> | ||
Line 1,796: | Line 2,679: | ||
</td> | </td> | ||
<td> | <td> | ||
- | + | 17/08/2010 | |
</td><td> | </td><td> | ||
''E. coli'' TOP10 | ''E. coli'' TOP10 | ||
Line 1,807: | Line 2,690: | ||
725 bp | 725 bp | ||
</td> | </td> | ||
- | <td> | + | <td>plux + RBS + luxI + TT in <partinfo>pSB4C5</partinfo></td> |
</tr> | </tr> | ||
Line 1,814: | Line 2,697: | ||
<td> | <td> | ||
I28 | I28 | ||
- | |||
- | |||
</td> | </td> | ||
<td> | <td> | ||
Line 1,821: | Line 2,702: | ||
</td> | </td> | ||
<td> | <td> | ||
- | + | 17/08/2010 | |
</td><td> | </td><td> | ||
''E. coli'' TOP10 | ''E. coli'' TOP10 | ||
Line 1,832: | Line 2,713: | ||
886 bp | 886 bp | ||
</td> | </td> | ||
- | <td> | + | <td>plux + RBS + lasI + terminator in <partinfo>pSB4C5</partinfo></td> |
</tr> | </tr> | ||
Line 1,852: | Line 2,733: | ||
<partinfo>pSB4C5</partinfo> | <partinfo>pSB4C5</partinfo> | ||
</td><td> | </td><td> | ||
- | + | Seq ok (BMR), length ok. | |
</td> | </td> | ||
<td> | <td> | ||
904 bp | 904 bp | ||
</td> | </td> | ||
- | <td> | + | <td>constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo></td> |
</tr> | </tr> | ||
Line 1,876: | Line 2,757: | ||
<partinfo>pSB4C5</partinfo> | <partinfo>pSB4C5</partinfo> | ||
</td><td> | </td><td> | ||
- | + | Seq ok (BMR), length ok. | |
</td> | </td> | ||
<td> | <td> | ||
904 bp | 904 bp | ||
</td> | </td> | ||
- | <td> | + | <td>constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo></td> |
</tr> | </tr> | ||
Line 1,905: | Line 2,786: | ||
904 bp | 904 bp | ||
</td> | </td> | ||
- | <td> | + | <td>constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo></td> |
</tr> | </tr> | ||
Line 1,930: | Line 2,811: | ||
904 bp | 904 bp | ||
</td> | </td> | ||
- | <td> | + | <td>constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo></td> |
</tr> | </tr> | ||
Line 1,954: | Line 2,835: | ||
904 bp | 904 bp | ||
</td> | </td> | ||
- | <td> | + | <td>constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo></td> |
</tr> | </tr> | ||
Line 1,978: | Line 2,859: | ||
904 bp | 904 bp | ||
</td> | </td> | ||
- | <td> | + | <td>constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo></td> |
</tr> | </tr> | ||
Line 2,002: | Line 2,883: | ||
904 bp | 904 bp | ||
</td> | </td> | ||
- | <td> | + | <td>constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo></td> |
</tr> | </tr> | ||
Line 2,023: | Line 2,904: | ||
<partinfo>pSB4C5</partinfo> | <partinfo>pSB4C5</partinfo> | ||
</td><td> | </td><td> | ||
- | + | Seq ok (BMR), length ok. | |
</td> | </td> | ||
<td> | <td> | ||
904 bp | 904 bp | ||
</td> | </td> | ||
- | <td> | + | <td>constitutive promoter + RBS + mRFP1 + TT in <partinfo>pSB4C5</partinfo></td> |
</tr> | </tr> | ||
- | |||
Latest revision as of 14:30, 27 October 2010
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