Team:Newcastle/8 September 2010

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=Characterization of filamentous cell part=
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===Gel extraction===
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=Hyperspankoid characterisation=
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==Aim==
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===Gel extraction===
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====Aims====
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The aim of this experiment is to prepare slides for the ''Bacillus subtilis'' 168 cells which have our filamentous cell part on the chromsome and are under ''lacI'' regulation from pMutin4.
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Gel extraction of the four sequences that were amplified and cut with ''Eco''R1[[Team:Newcastle/7_September_2010#Hyperspankoid_characterisation| yesterday]] was carried out today.  
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====Materials and Protocol====
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==Materials and Protocol==
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Please refer to [[Team:Newcastle/Gel_extraction| Gel extraction]] for the gel extraction protocol.
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====Results, Discussion and Conclusion====
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Please refer to: [[Team:Newcastle/IPTG INduction|Slide preparation for IPTG induced cells]] for materials and protocol.
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Today we see the cells on a flourescent microscope.
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==Result and Conclusion==
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Please refer to: [[Team:Newcastle/Filamentous_Cells#Characterisation|Characterisation]] for the result.
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'''Go back to our main [[Team:Newcastle/notebook| Lab book]] page'''
 
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Latest revision as of 02:41, 28 October 2010

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Contents

Characterization of filamentous cell part

Aim

The aim of this experiment is to prepare slides for the Bacillus subtilis 168 cells which have our filamentous cell part on the chromsome and are under lacI regulation from pMutin4.

Materials and Protocol

Please refer to: Slide preparation for IPTG induced cells for materials and protocol.

Today we see the cells on a flourescent microscope.

Result and Conclusion

Please refer to: Characterisation for the result.


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