Team:Newcastle/6 September 2010

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=''roc''F=
 
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===Aims===
 
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''roc''F miniprep: we aim to purify ''roc''F in p.....
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=Checking for transformants=
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===Materials and protocol===
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Today we checked for transformants from the filamentous ''Bacillus subtilis'' transformation (with pMutin4) we performed yesterday.
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[[Team:Newcastle/Qiagen_Minipreps#Plasmid_extraction|See Mini prep protocol]]
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===Results===
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====Nanodrop====
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''roc''F
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Concentration of DNA in ng/µl
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#93.1
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#102.0
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Subtilin Immunity BioBrick
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== Aims ==
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Overnight cultures of all the colonies from the 'SI 1/9/10' plates was carried out today. 
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== Methods ==
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The [[Team:Newcastle/Growing_an_overnight_cultures| Growing Overnight Cultures]] protocol was followed. They were grown on LB with chloramphenicol. Altogether 16 colonies were produced on the plates and were left overnight. 
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=Sucrose/Levan's glue=
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The sucrose cultures and controls were compared. The cultures grown on sucrose should be considerably thicker.
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The overnight cultures of the different strains were also grown on solid agar plates with and without sucrose.  
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===PHOTOS===
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We checked our transformants under the light microscope, expecting to see a non-filamentous phenotype since the cells had not been induced with IPTG. The resutls were as expected.
{{Team:Newcastle/footer}}
{{Team:Newcastle/footer}}

Latest revision as of 02:36, 28 October 2010

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Checking for transformants

Today we checked for transformants from the filamentous Bacillus subtilis transformation (with pMutin4) we performed yesterday.

We checked our transformants under the light microscope, expecting to see a non-filamentous phenotype since the cells had not been induced with IPTG. The resutls were as expected.

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