Team:UC Davis/protocols/culture.html

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               <p> You will need: <br />
               <p> You will need: <br />
                 <ul>
                 <ul>
-
                 <li>Luria broth</li>
+
                 <li><a href="https://2010.igem.org/Team:UC_Davis/protocols/luriabroth.html" class="help">Luria broth</a></li>
                 <li>Pasteur pipette</li>
                 <li>Pasteur pipette</li>
                 <li>Pipetting Aid</li>
                 <li>Pipetting Aid</li>
                 <li>Sterilized polypropylene tubes</li>
                 <li>Sterilized polypropylene tubes</li>
                 <li>Microtiter pipette & small sterilized pipette tips</li>
                 <li>Microtiter pipette & small sterilized pipette tips</li>
-
                 <li>Antibiotic stock </li>
+
                 <li>Antibiotic resistance stock </li>
                 <li>Plates with the desired microbe to be cultured </li>
                 <li>Plates with the desired microbe to be cultured </li>
                 </ul><p>
                 </ul><p>
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<ul>
<ul>
<li>Light bunsen burner.</li>
<li>Light bunsen burner.</li>
-
<li>Pour 4 mL of luria broth into each polypropylene tube. </li>
+
<li>Pour 4 mL of <a href="https://2010.igem.org/Team:UC_Davis/protocols/luriabroth.html" class="help">luria broth</a> into each polypropylene tube. </li>
<li>Pipette the required amount of antibiotic resistance into each tube.  Recommended amounts can be looked up on <a href="http://www.openwetware.org/wiki/Main_Page" class="help">openwetware.org</a>. </li>
<li>Pipette the required amount of antibiotic resistance into each tube.  Recommended amounts can be looked up on <a href="http://www.openwetware.org/wiki/Main_Page" class="help">openwetware.org</a>. </li>
<li>Use a small pipette tip to lightly touch one isolated colony, then place into the respected tube. </li>
<li>Use a small pipette tip to lightly touch one isolated colony, then place into the respected tube. </li>
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<a name="references"><h1>References</h1></a>
<a name="references"><h1>References</h1></a>
<ul>
<ul>
-
<li>Notes by David Larsen 9/11/08 </li>
+
<li>Notes by David Larsen </li>
 +
<li><a href="http://www.openwetware.org/wiki/Main_Page">Open Wetware</a></li>
</ul><p>                               
</ul><p>                               
         </td>
         </td>

Latest revision as of 00:12, 8 September 2010

Culture

Materials

You will need:

  • Luria broth
  • Pasteur pipette
  • Pipetting Aid
  • Sterilized polypropylene tubes
  • Microtiter pipette & small sterilized pipette tips
  • Antibiotic resistance stock
  • Plates with the desired microbe to be cultured

Procedure

  • Light bunsen burner.
  • Pour 4 mL of luria broth into each polypropylene tube.
  • Pipette the required amount of antibiotic resistance into each tube. Recommended amounts can be looked up on openwetware.org.
  • Use a small pipette tip to lightly touch one isolated colony, then place into the respected tube.
  • Close tubes and then incubate at 37°C for approximately 12 to 16 hours.

Purpose

To grow the desired microbe in slightly larger quantities.

References

We would like to take a moment to thank all of our sponsors for their very generous donations, as we could not have done this without your help!

We would also like to thank and acknowledge:
Our Advisors
Marc Facciotti
Ilias Tagkopoulos
Technical Guidance
David Larsen
Andrew Yao
Visiting iGEMer
Jia Li of Zhejiang University (TEAM ZJU-China)
cI Promoter Screen
Drew Endy - Stanford
Thomas Schneider - NIH
Want to sponsor us? Send an email to mtfacciotti@ucdavis.edu to discuss various ways you can help! :)

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