Team:UC Davis/protocols/luriabroth.html

From 2010.igem.org

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<p class="header"><b>Luria Broth</b></a><p class="small">By David Larsen</a><p>
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<p class="header"><b>Luria Broth</b></a><p>
<a name="materials"><h1>Materials</h1></a>
<a name="materials"><h1>Materials</h1></a>
               <p> You will need: <br />
               <p> You will need: <br />
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<li>Aliquot into smaller glass bottles. (Optional) </li>
<li>Aliquot into smaller glass bottles. (Optional) </li>
<li>Autoclave. </li>
<li>Autoclave. </li>
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</ul>
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<a name="purpose"><h1>Purpose</h1></a>
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For <a href="https://2010.igem.org/Team:UC_Davis/protocols/culture.html" class="help">culturing</a> desired microbes. <p>
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<a name="references"><h1>References</h1></a>
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<ul>
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<li>Written by David Larsen 9/11/08</li>
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<li>Cold Spring Harbor protocol </li>
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<li><a href="#materials" class="help">Materials</a></li>
<li><a href="#materials" class="help">Materials</a></li>
<li><a href="#procedure" class="help">Procedure</a></li>
<li><a href="#procedure" class="help">Procedure</a></li>
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<li><a href="#purpose" class="help">Purpose</a></li>
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<li><a href="#references" class="help">References</a></li>
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Latest revision as of 20:45, 9 September 2010

Luria Broth

Materials

You will need:

  • Deionized water
  • Tryptone
  • NaCl
  • Yeast Extract

Procedure

  • Combine 10g tryptone, 5g yeast extract, 10g NaCl, and 950 mL of deionized water into a large beaker/Erlenmeyer flask.
  • Mix until dissolved.
  • Check to see if the pH is 7.0 using a pH meter. If needed, add 5N NaOH to adjust the pH to 7.0.
  • Fill solution to 1L with deionized water.
  • Aliquot into smaller glass bottles. (Optional)
  • Autoclave.

Purpose

For culturing desired microbes.

References

  • Written by David Larsen 9/11/08
  • Cold Spring Harbor protocol

We would like to take a moment to thank all of our sponsors for their very generous donations, as we could not have done this without your help!

We would also like to thank and acknowledge:
Our Advisors
Marc Facciotti
Ilias Tagkopoulos
Technical Guidance
David Larsen
Andrew Yao
Visiting iGEMer
Jia Li of Zhejiang University (TEAM ZJU-China)
cI Promoter Screen
Drew Endy - Stanford
Thomas Schneider - NIH
Want to sponsor us? Send an email to mtfacciotti@ucdavis.edu to discuss various ways you can help! :)

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