Team:Newcastle/2 September 2010

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{{Team:Newcastle/mainbanner}}
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=pH changing=
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=Preparation for pH acclimatisation of ''Bacillus subtilis'' 168=
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We brought hepes (which is similar to homopipes) to pH 7 then autoclaved it.
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We prepared 100mM of HEPES (it is a dibasic compound) at pH 7.0 and sent it for autoclaving.
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=Preparing medium for natto=
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We prepared antibitoic ,medium for natto: 17.5g of broth in 1L, so 1.75 in 100ml
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=Extreme Overnight cultures for Gibson=
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# G1 (T1) 1 Colony 1,
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# G1 (T2) 7 Colonies 2-6
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# G2 (T3) 2 Colonies 9,10
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# G2 (T4) 14 Colonies 11-24
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# G3 (T5) 3 Colonies 25-27
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# G3 (T6) 2 Colonies 28-29
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# G4 (T7) 4 Colonies 30-33
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# G4 (T8) 9 Colonies 34-42
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# G5 (T9) 0  
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# G5 (T10) 2 Colonies 43-44
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=Gibson for ''rocF''=
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*Promoter 136bp - 0.7 microl
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* rocF 1 246bp - 1.2 microl
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* rocF 2 597bp - 1.9 microl
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* rocF 3 125bp - 0.4 microl
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* DTERM 116bp - 0.8microl
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Total: 1190bp 
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{{Team:Newcastle/footer}}
{{Team:Newcastle/footer}}

Latest revision as of 01:53, 28 October 2010

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Preparation for pH acclimatisation of Bacillus subtilis 168

We prepared 100mM of HEPES (it is a dibasic compound) at pH 7.0 and sent it for autoclaving.

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