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Team:LMU-Munich/Notebook/Protocols/17 Special thermal cycler programs/Touch down
From 2010.igem.org
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Latest revision as of 14:40, 4 September 2010
PCR program: Touch-Down
95°C
1 min
95°C
30 sec
56°C
60 sec
72°C
30 sec
return to step 2 for 2 cycles
95°C
30 sec
54°C
60 sec
72°C
30 sec
return to step 6 for 2 cycles
95°C
30 sec
52°C
60 sec
72°C
30 sec
return to step 10 for 2 cycles
95°C
30 sec
50°C
60 sec
72°C
30 sec
return to step 14 for 2 cycles
95°C
30 sec
48°C
60 sec
72°C
30 sec
return to step 18 for 2 cycles
95°C
30 sec
46°C
60 sec
72°C
30 sec
return to step 22 for 2 cycles
95°C
30 sec
44°C
60 sec
72°C
30 sec
return to step 26 for 2 cycles
95°C
30 sec
42°C
60 sec
72°C
30 sec
return to step 30 for 2 cycles
95°C
30 sec
48°C
60 sec
72°C
30 sec
return to step 34 for 30 cycles
72°C
10 min
12°C
forever
