Team:Lethbridge/Notebook/Working Plasmids
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+ | <hr> | ||
+ | <BLOCKQUOTE> | ||
+ | |||
Back to [[Team:Lethbridge/Notebook|Notebook]]<br><br> | Back to [[Team:Lethbridge/Notebook|Notebook]]<br><br> | ||
- | <b>Updated | + | <b>Updated June 1/2010</b><br><br> |
<b>Row A</b> | <b>Row A</b> | ||
<table><table border="3"> | <table><table border="3"> | ||
- | <tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration (µg/mL)</td></tr> | + | <tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration via<br> Spectroscopy(µg/mL)</td><td>Concentration via <br>gel quantification (µg/mL)</td></tr> |
- | <tr><td>A1</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>A1</td><td>sRBS-Lum-dT(2)</td><td></td><td>May 13/2010 (JV)</td><td>965</td><td></td></tr> |
- | <tr><td>A2</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>A2</td><td>sRBS-Lum-dT(1)</td><td></td><td>May 13/2010 (JV)</td><td>1145</td><td></td></tr> |
- | <tr><td>A3</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>A3</td><td>Lumazine</td><td></td><td>May 13/2010 (AV)</td><td>750</td><td></td></tr> |
- | <tr><td>A4</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>A4</td><td>pBad(1)</td><td></td><td>May 13/2010 (AV)</td><td>1320</td><td></td></tr> |
- | <tr><td>A5</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>A5</td><td>pBad(2)</td><td></td><td>May 13/2010 (JV)</td><td>2050</td><td></td></tr> |
- | <tr><td>A6</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>A6</td><td>sRBS</td><td></td><td>May 15/2010</td><td>6970</td><td></td></tr> |
- | <tr><td>A7</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>A7</td><td>sRBS</td><td></td><td>May 15/2010</td><td>17000</td><td></td></tr> |
- | <tr><td>A8</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>A8</td><td>CFP Complete</td><td></td><td>Feb 20/2010</td><td>1395</td><td></td></tr> |
- | <tr><td>A9</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>A9</td><td>pLacI</td><td></td><td>Feb 20/2010</td><td>990</td><td></td></tr> |
- | <tr><td>A10</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>A10</td><td>sRBS</td><td></td><td>May 18/2010</td><td>1765</td><td></td></tr> |
</table><br> | </table><br> | ||
- | <b>Row | + | <b>Row B</b> |
<table><table border="3"> | <table><table border="3"> | ||
- | <tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration (µg/mL)</td></tr> | + | <tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration via<br> Spectroscopy(µg/mL)</td><td>Concentration via <br>gel quantification (µg/mL)</td></tr> |
- | <tr><td> | + | <tr><td>B1</td><td>EYFP</td><td></td><td>May 13/2010</td><td>1370</td><td></td></tr> |
- | <tr><td> | + | <tr><td>B2</td><td>N-term tag</td><td></td><td>May 13/2010</td><td>415</td><td></td></tr> |
- | <tr><td> | + | <tr><td>B3</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td> | + | <tr><td>B4</td><td>pSB-NEYFP</td><td></td><td>May 13/2010</td><td>1185</td><td></td></tr> |
- | <tr><td> | + | <tr><td>B5</td><td>pSB-CEYFP</td><td></td><td>May 13/2010</td><td>1055</td><td></td></tr> |
- | <tr><td> | + | <tr><td>B6</td><td>CFP Complete</td><td></td><td>May 13/2010</td><td>1285</td><td></td></tr> |
- | <tr><td> | + | <tr><td>B7</td><td>sRBS-Lum-dT</td><td></td><td>May 13/2010</td><td>4375</td><td></td></tr> |
- | <tr><td> | + | <tr><td>B8</td><td>sRBS-Lum-dT</td><td></td><td>Feb 20/2010</td><td>4780</td><td></td></tr> |
- | <tr><td> | + | <tr><td>B9</td><td>mr. Gene mms6</td><td></td><td>Feb 20/2010</td><td>2455</td><td></td></tr> |
- | <tr><td> | + | <tr><td>B10</td><td>pBad-TetR</td><td></td><td>May 18/2010</td><td>2915</td><td></td></tr> |
</table><br> | </table><br> | ||
- | <b>Row | + | <b>Row C</b> |
<table><table border="3"> | <table><table border="3"> | ||
- | <tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration (µg/mL)</td></tr> | + | <tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration via<br> Spectroscopy(µg/mL)</td><td>Concentration via <br>gel quantification (µg/mL)</td></tr> |
- | <tr><td> | + | <tr><td>C1</td><td>dT</td><td></td><td></td><td>2915</td><td></td></tr> |
- | <tr><td> | + | <tr><td>C2</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td> | + | <tr><td>C3</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td> | + | <tr><td>C4</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td> | + | <tr><td>C5</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td> | + | <tr><td>C6</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td> | + | <tr><td>C7</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td> | + | <tr><td>C8</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td> | + | <tr><td>C9</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td> | + | <tr><td>C10</td><td></td><td></td><td></td><td></td><td></td></tr> |
</table><br> | </table><br> | ||
- | <b>Row | + | <b>Row D</b> |
<table><table border="3"> | <table><table border="3"> | ||
- | <tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration (µg/mL)</td></tr> | + | <tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration via<br> Spectroscopy(µg/mL)</td><td>Concentration via <br>gel quantification (µg/mL)</td></tr> |
- | <tr><td> | + | <tr><td>D1</td><td>pLacI</td><td></td><td></td><td>70</td><td></td></tr> |
- | <tr><td> | + | <tr><td>D2</td><td>sRBS-Lum-dT</td><td></td><td></td><td>95</td><td></td></tr> |
- | <tr><td> | + | <tr><td>D3</td><td>C-term (H4)</td><td></td><td></td><td>860</td><td></td></tr> |
- | <tr><td> | + | <tr><td>D4</td><td>C-term (I4)</td><td></td><td></td><td>1145</td><td></td></tr> |
- | <tr><td> | + | <tr><td>D5</td><td>C-term (J4)</td><td></td><td></td><td>1805</td><td></td></tr> |
- | <tr><td> | + | <tr><td>D6</td><td>pLacI (B1)</td><td></td><td></td><td>440</td><td></td></tr> |
- | <tr><td> | + | <tr><td>D7</td><td>xylE (B4)</td><td></td><td></td><td>1820</td><td></td></tr> |
- | <tr><td> | + | <tr><td>D8</td><td>xylE (C4)</td><td></td><td></td><td>420</td><td></td></tr> |
- | <tr><td> | + | <tr><td>D9</td><td>mms6 (A6)</td><td></td><td></td><td>1530</td><td></td></tr> |
- | <tr><td> | + | <tr><td>D10</td><td>mms6 (B6)</td><td></td><td></td><td>1440</td><td></td></tr> |
</table><br> | </table><br> | ||
- | <b>Row | + | <b>Row E</b> |
<table><table border="3"> | <table><table border="3"> | ||
- | <tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration (µg/mL)</td></tr> | + | <tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration via<br> Spectroscopy(µg/mL)</td><td>Concentration via <br>gel quantification (µg/mL)</td></tr> |
- | <tr><td> | + | <tr><td>E1</td><td>NEYFP</td><td></td><td></td><td>235</td><td></td></tr> |
- | <tr><td> | + | <tr><td>E2</td><td>NEYFP</td><td></td><td></td><td>2980</td><td></td></tr> |
- | <tr><td> | + | <tr><td>E3</td><td>Fusion CEYFP</td><td></td><td></td><td>255</td><td></td></tr> |
- | <tr><td> | + | <tr><td>E4</td><td>Fusion CEYFP</td><td></td><td></td><td>490</td><td></td></tr> |
- | <tr><td> | + | <tr><td>E5</td><td>Fusion CEYFP</td><td></td><td></td><td>335</td><td></td></tr> |
- | <tr><td> | + | <tr><td>E6</td><td>CEYFP</td><td></td><td></td><td>1605</td><td></td></tr> |
- | <tr><td> | + | <tr><td>E7</td><td>CEYFP</td><td></td><td></td><td>1930</td><td></td></tr> |
- | <tr><td> | + | <tr><td>E8</td><td>EYFP</td><td></td><td></td><td>640</td><td></td></tr> |
- | <tr><td> | + | <tr><td>E9</td><td>EYFP</td><td></td><td></td><td>605</td><td></td></tr> |
- | <tr><td> | + | <tr><td>E10</td><td>EYFP</td><td></td><td></td><td>620</td><td></td></tr> |
</table><br> | </table><br> | ||
- | <b>Row | + | <b>Row F</b> |
<table><table border="3"> | <table><table border="3"> | ||
- | <tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration (µg/mL)</td></tr> | + | <tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration via<br> Spectroscopy(µg/mL)</td><td>Concentration via <br>gel quantification (µg/mL)</td></tr> |
- | <tr><td> | + | <tr><td>F1</td><td>ECFP</td><td></td><td></td><td>470</td><td></td></tr> |
- | <tr><td> | + | <tr><td>F2</td><td>ECFP</td><td></td><td></td><td>1300</td><td></td></tr> |
- | <tr><td> | + | <tr><td>F3</td><td>ECFP</td><td></td><td></td><td>2010</td><td></td></tr> |
- | <tr><td> | + | <tr><td>F4</td><td>pBad-TetR</td><td></td><td></td><td>400</td><td></td></tr> |
- | <tr><td> | + | <tr><td>F5</td><td>pBad-TetR</td><td></td><td></td><td>575</td><td></td></tr> |
- | <tr><td> | + | <tr><td>F6</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td> | + | <tr><td>F7</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td> | + | <tr><td>F8</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td> | + | <tr><td>F9</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td> | + | <tr><td>F10</td><td></td><td></td><td></td><td></td><td></td></tr> |
</table><br> | </table><br> | ||
<b>Row G</b> | <b>Row G</b> | ||
<table><table border="3"> | <table><table border="3"> | ||
- | <tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration (µg/mL)</td></tr> | + | <tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration via<br> Spectroscopy(µg/mL)</td><td>Concentration via <br>gel quantification (µg/mL)</td></tr> |
- | <tr><td>G1</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>G1</td><td>EYFP</td><td></td><td></td><td>1300</td><td></td></tr> |
- | <tr><td>G2</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>G2</td><td>sRBS-Lum-dT(1)</td><td></td><td></td><td>335</td><td></td></tr> |
- | <tr><td>G3</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>G3</td><td>sRBS-Lum-dT(2)</td><td></td><td></td><td>540</td><td></td></tr> |
- | <tr><td>G4</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>G4</td><td>pSB-CEYFP</td><td></td><td></td><td>340</td><td></td></tr> |
- | <tr><td>G5</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>G5</td><td>CFP Complete</td><td></td><td></td><td>355</td><td></td></tr> |
- | <tr><td>G6</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>G6</td><td>pBad(1)</td><td></td><td></td><td>175</td><td></td></tr> |
- | <tr><td>G7</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>G7</td><td>pBad(2)</td><td></td><td></td><td>315</td><td></td></tr> |
- | <tr><td>G8</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>G8</td><td>N-term tag</td><td></td><td></td><td>270</td><td></td></tr> |
- | <tr><td>G9</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>G9</td><td>Lumazine</td><td></td><td></td><td>265</td><td></td></tr> |
- | <tr><td>G10</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>G10</td><td></td><td></td><td></td><td></td><td></td></tr> |
</table><br> | </table><br> | ||
<b>Row H</b> | <b>Row H</b> | ||
<table><table border="3"> | <table><table border="3"> | ||
- | <tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration (µg/mL)</td></tr> | + | <tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration via<br> Spectroscopy(µg/mL)</td><td>Concentration via <br>gel quantification (µg/mL)</td></tr> |
- | <tr><td>H1</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>H1</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td>H2</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>H2</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td>H3</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>H3</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td>H4</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>H4</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td>H5</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>H5</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td>H6</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>H6</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td>H7</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>H7</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td>H8</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>H8</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td>H9</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>H9</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td>H10</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>H10</td><td></td><td></td><td></td><td></td><td></td></tr> |
</table><br> | </table><br> | ||
<b>Row I</b> | <b>Row I</b> | ||
<table><table border="3"> | <table><table border="3"> | ||
- | <tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration (µg/mL)</td></tr> | + | <tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration via<br> Spectroscopy(µg/mL)</td><td>Concentration via <br>gel quantification (µg/mL)</td></tr> |
- | <tr><td>I1</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>I1</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td>I2</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>I2</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td>I3</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>I3</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td>I4</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>I4</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td>I5</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>I5</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td>I6</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>I6</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td>I7</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>I7</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td>I8</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>I8</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td>I9</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>I9</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td>I10</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>I10</td><td></td><td></td><td></td><td></td><td></td></tr> |
</table><br> | </table><br> | ||
<b>Row J</b> | <b>Row J</b> | ||
<table><table border="3"> | <table><table border="3"> | ||
- | <tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration (µg/mL)</td></tr> | + | <tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration via<br> Spectroscopy(µg/mL)</td><td>Concentration via <br>gel quantification (µg/mL)</td></tr> |
- | <tr><td>J1</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>J1</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td>J2</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>J2</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td>J3</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>J3</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td>J4</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>J4</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td>J5</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>J5</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td>J6</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>J6</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td>J7</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>J7</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td>J8</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>J8</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td>J9</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>J9</td><td></td><td></td><td></td><td></td><td></td></tr> |
- | <tr><td>J10</td><td></td><td></td><td></td><td></td></tr> | + | <tr><td>J10</td><td></td><td></td><td></td><td></td><td></td></tr> |
</table><br> | </table><br> |