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Team:Stockholm/25 July 2010
From 2010.igem.org
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Nina
Inoculation of IgG protease
The dish had 5 colonies, I inoculated these in 12 ml LB with 24 ul chloramphenocol resistance (50mg/ml). When I know which colony is suitable I will miniprep it and send in to iGEM hq.
Mimmi
LMWP
Re-doing the PCR
- 25 colonies were checked
- Nina and Johan were running the PCR, I made the gels...
Gels
| well | sample |
|---|---|
| 1 | ladder |
| 2 | 1control |
| 3 | 3 LMWP C |
| 4 | 4 LMWP C |
| 5 | 5 LMWP C (A) |
| 6 | 6 LMWP C (B) |
| 7 | 7 LMWP C (C) |
| 8 | |
| 9 | 9 LMWP N (C) |
| well | sample | well | sample |
|---|---|---|---|
| 1 | ladder | 12 | ladder |
| 2 | 10 LMWP N (B) | 13 | empty |
| 3 | 11 LMWP N (A) | 14 | 18 LMWP N |
| 4 | 12 LMWP N | 15 | empty |
| 5 | 13 LMWP N | 16 | empty |
| 6 | 14 LMWP N | 17 | 19 LMWP N |
| 7 | 15 LMWP N | 18 | 20 LMWP N |
| 8 | 16 LMWP N | 19 | 21 LMWP N |
| 9 | 17 LMWP N | 20 | 22 LMWP N |
| 10 | empty | 21 | 23 LMWP N |
| 11 | 25 LMWP N | 22 | 24 LMWP N |
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