Team:UT-Tokyo/Sudoku experiment

From 2010.igem.org

(Difference between revisions)
(New page: {{UT-Tokyo_HEADER}} <div id="main_all"> = '''Sudoku''' = [https://2010.igem.org/Team:UT-Tokyo/Sudoku_abstract Abstract] [https://2010.igem.org/Team:UT-Tokyo/Sudoku_construct Constru...)
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[https://2010.igem.org/Team:UT-Tokyo/Sudoku_abstract  Abstract]     
[https://2010.igem.org/Team:UT-Tokyo/Sudoku_abstract  Abstract]     
[https://2010.igem.org/Team:UT-Tokyo/Sudoku_construct  Construct]     
[https://2010.igem.org/Team:UT-Tokyo/Sudoku_construct  Construct]     
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[https://2010.igem.org/Team:UT-Tokyo/Sudoku_experiment  Experiment]   
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Lab note     
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[https://2010.igem.org/Team:UT-Tokyo/Sudoku_note  Note]   
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[https://2010.igem.org/Team:UT-Tokyo/Sudoku_result  Result]
[https://2010.igem.org/Team:UT-Tokyo/Sudoku_result  Result]
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== '''Experiment''' ==
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== '''Lab note''' ==
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This is an abstract page.
 
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If you can see detail data, please click each protocols.
 
This is an abstract page.
This is an abstract page.
If you can see detail data, please click each protocols.
If you can see detail data, please click each protocols.
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[[Image:Sudoku_const_Pre.png|200px|thumb|Pre-experiment]]
[[Image:Sudoku_const_Pre.png|200px|thumb|Pre-experiment]]
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=== [https://2010.igem.org/Team:UT-Tokyo/Sudoku_data  Make construct] ===
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=== Make construct ===
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0,i,ii,iii
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Detail protocols about this is:
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[https://2010.igem.org/Team:UT-Tokyo/Sudoku_notebook_6  June]
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[https://2010.igem.org/Team:UT-Tokyo/Sudoku_notebook  July]
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[https://2010.igem.org/Team:UT-Tokyo/Sudoku_notebook_2  August]
=== Check test ===
=== Check test ===
Line 75: Line 78:
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J31006  BBa_J31006]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J31006  BBa_J31006]
  || plate1-1N || pSB1A2 || 1191bp
  || plate1-1N || pSB1A2 || 1191bp
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|-
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| 12 || hixC ||
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_J44000  BBa_J44000]
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|| plate1-1B || pSB1A2 || 26bp
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|-
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| 13 || --- ||
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---
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|| --- || --- || ---
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|-
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| 14 || lox66 ||
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_I718016  BBa_I718016]
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|| plate1-17H || pSB1A2 || 34bp
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|-
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| 15 || rbs-mRFP1-terminator ||
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_I13507  BBa_I13507]
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|| plate1-22O || pSB1A2 || 861bp
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|-
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| 16 || location sequence(old) ||
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order
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|| --- || --- || ---
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|-
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| 17 || pSP6(rev) ||
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order
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|| --- || --- || ---
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|-
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| 18 || lox2272 ||
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order
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|| --- || --- || ---
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|-
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| 19 || loading sequence(rev) ||
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order
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|| --- || --- || ---
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|-
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| 20 || frt ||
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[http://partsregistry.org/Part:BBa_J61020  BBa_J61020]
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|| From HQ || pSB1A2 || 34bp
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|-
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| 21 || hin(true) ||
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[http://partsregistry.org/Part:BBa_J31000  BBa_J31000]
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|| From HQ || ??? || 573bp
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|-
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| 22 || hin(rev) ||
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---
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|| --- || ??? || 573bp
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|-
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| 23 || flpe ||
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---
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|| --- || --- || about 1.2kbp
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|-
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| 24 || gfp unit ||
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---
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|| --- || --- || about 1.2kbp
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|-
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| 25 || flpe(rev) ||
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---
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|| --- || --- || about 1.2kbp
|}
|}
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== Main construct ==
== Main construct ==
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[[Image:Sudoku_const_Main_5.png|700px|Sudoku's main construct]]
=== Research of SP6 RNApol cDNA and F plasmid ===
=== Research of SP6 RNApol cDNA and F plasmid ===

Latest revision as of 01:56, 19 September 2010

Sudoku

Abstract Construct Lab note Result

Lab note

This is an abstract page. If you can see detail data, please click each protocols.

Pre-experiment

Pre-experiment

Make construct

Detail protocols about this is:

June

July

August

Check test

Check test


Number Name Link to BioBrick Plate coordinate Vector Code length
1 T7 promoter

[http://partsregistry.org/wiki/index.php?title=Part:BBa_I712074 BBa_I712074]

plate1-6N pSB1AK8 46bp
2 rbs

[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0030 BBa_B0030]

plate1-1H pSB1A2 15bp
3 cre recombinase

[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61047 BBa_J61047]

plate1-5D pSB1A2 1037bp
4 double terminator

[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0014 BBa_B0014]

plate2-24C pSB1AK3 95bp
5 lox66 recombinase site

[http://partsregistry.org/wiki/index.php?title=Part:BBa_I718017 BBa_I718017]

plate1-17J pSB1A2 34bp
6 Kan resistance (rev)

[http://partsregistry.org/wiki/index.php?title=Part:BBa_J31002 BBa_J31002]

plate1-2K pSB1A2 816bp
7 rbs (rev)

[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0014 BBa_B0014]

plate1-1J pSB1A2 15bp
8 lox71 recombinase site

order-made

--- --- 34bp
9 single terminator

[http://partsregistry.org/wiki/index.php?title=Part:BBa_B1006 BBa_B1006]

plate1-4H pSB1AK3 39bp
10 constant express promoter

[http://partsregistry.org/wiki/index.php?title=Part:BBa_J23119 BBa_J23119]

plate1-18A pSB1A2 35bp
11 Tet resistance (rev)

[http://partsregistry.org/wiki/index.php?title=Part:BBa_J31006 BBa_J31006]

plate1-1N pSB1A2 1191bp
12 hixC

[http://partsregistry.org/wiki/index.php?title=Part:BBa_J44000 BBa_J44000]

plate1-1B pSB1A2 26bp
13 ---

---

--- --- ---
14 lox66

[http://partsregistry.org/wiki/index.php?title=Part:BBa_I718016 BBa_I718016]

plate1-17H pSB1A2 34bp
15 rbs-mRFP1-terminator

[http://partsregistry.org/wiki/index.php?title=Part:BBa_I13507 BBa_I13507]

plate1-22O pSB1A2 861bp
16 location sequence(old)

order

--- --- ---
17 pSP6(rev)

order

--- --- ---
18 lox2272

order

--- --- ---
19 loading sequence(rev)

order

--- --- ---
20 frt

[http://partsregistry.org/Part:BBa_J61020 BBa_J61020]

From HQ pSB1A2 34bp
21 hin(true)

[http://partsregistry.org/Part:BBa_J31000 BBa_J31000]

From HQ  ??? 573bp
22 hin(rev)

---

---  ??? 573bp
23 flpe

---

--- --- about 1.2kbp
24 gfp unit

---

--- --- about 1.2kbp
25 flpe(rev)

---

--- --- about 1.2kbp

Main construct

Sudoku's main construct

Research of SP6 RNApol cDNA and F plasmid

Research of SP6 RNApol cDNA and F plasmid

Convert lox71(rev) to lox66(rev)

Convert lox71(rev) to lox66(rev)

Make Hin(rev)

Make Hin(rev)

Revise MS2 gene

Revise MS2 gene

Make location(rev)-rbs(rev)-location(rev)

Make location(rev)-rbs(rev)-location(rev)


Copyright © 2010 iGEM UT-Tokyo. All rights reserved.

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