Team:Washington/Project/Safety/Secretion
From 2010.igem.org
(Difference between revisions)
(New page: __NOTOC__ {{Template:Team:Washington/Templates/Header}} <html> <script type="text/javascript"> $(function() { var mainimg = document.getElementById("mainimg"); var default_src = mainim...) |
m |
||
(9 intermediate revisions not shown) | |||
Line 25: | Line 25: | ||
</html> | </html> | ||
<!---------------------------------------PAGE CONTENT GOES BELOW THIS----------------------------------------> | <!---------------------------------------PAGE CONTENT GOES BELOW THIS----------------------------------------> | ||
- | + | Our work on Gram-negative Therapeutics was done only on non-pathogenic strains. We used the Type VI Secretion System from ''Pseudomonas aeruginosa'' PAO1. All modifications and testing of plasmids and fosmids were in non-pathogenic ''E. Coli'' strains, namely EPI300, SW102, and BL21. All cloning and testing of Tse2 was done in the non-pathogenic ''E. coli'' strains DH5a, MG1655, and BL21. | |
+ | The toxin we work with, Tse2, targets only prokaryotic cells, thus posing no direct danger to humans. At no time was a working type six secretion system placed into ''E. coli'' producing Tse2. Therefore, none of the strains could cause any possible ecological danger. | ||
+ | <!---------------------------------------PAGE CONTENT GOES ABOVE THIS----------------------------------------> | ||
- | + | <div style="text-align:center"> | |
- | < | + | '''← [[Team:Washington/Project/Safety/Anthrax|Anthrax Work]]''' |
+ | | ||
+ | ''' [[Team:Washington/Team|Meet the Team]] →''' | ||
+ | </div> | ||
{{Template:Team:Washington/Templates/Footer}} | {{Template:Team:Washington/Templates/Footer}} |
Latest revision as of 00:17, 14 September 2010
Our work on Gram-negative Therapeutics was done only on non-pathogenic strains. We used the Type VI Secretion System from Pseudomonas aeruginosa PAO1. All modifications and testing of plasmids and fosmids were in non-pathogenic E. Coli strains, namely EPI300, SW102, and BL21. All cloning and testing of Tse2 was done in the non-pathogenic E. coli strains DH5a, MG1655, and BL21. The toxin we work with, Tse2, targets only prokaryotic cells, thus posing no direct danger to humans. At no time was a working type six secretion system placed into E. coli producing Tse2. Therefore, none of the strains could cause any possible ecological danger.