Team:Washington

From 2010.igem.org

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<img id="mainimg" src="/wiki/images/4/41/Main_graphic.png" alt="IPP System Overview" usemap="#cellmap" />
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<!---------------------------------------PAGE CONTENT GOES BELOW HERE---------------------------------------->
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While vital to our quality of life, traditional antibiotics face the serious
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problems of widespread bacterial resistance and destruction of natural gut
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flora - problems which call for improved twenty-first century antibiotics.
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Using synthetic biology tools, we designed, built, and tested two new
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systems to fight infections by both broad types of bacteria - Gram-positive
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and Gram-negative.  Our first project targets ''Bacillus anthracis'', the
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Gram-positive pathogen that causes anthrax.  We re-engineered an enzyme to
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remove the pathogen's protective coating, rendering it defenseless against
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the immune system.  In our second project, we re-engineered and transplanted
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a protein secretion system capable of combating Gram-negative bacteria into
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''E. coli''.  This system was designed to target Gram-negative pathogens in a
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modular and controllable fashion.  These two systems are the vanguard of a
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new era of antibiotics using the power of nature harnessed with the tools of
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synthetic biology.
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=== The Idealized Protein Purification System: Improving the lives of molecular biologists ===
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'''[[Team:Washington/Gram_Negative|Gram(-) Therapeutic]]'''
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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'''[[Team:Washington/Gram_Positive|Gram(+) Therapeutic]]'''
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Recombinant, purified protein production is a decades-old technology that has revolutionized research in biotechnology and medicine.  However, the traditional method of purified protein production is a time-consuming and laborious procedure requiring expensive and specialized equipment.  Our project, the Idealized Protein Purification (IPP) system, is an all-in-one protein expression and purification platform built on BioBrick standards that will reduce costs, save time, and simplify procedures associated with recombinant protein production.  The key to our IPP system is a novel combination of three subsystems: expression, secretion and display.  We use ''E. coli'' bacteria that we have genetically modified to be a chassis for expressing your favorite protein, secreting it to the media, then binding and displaying the protein on the cell surface.  At this point, collecting your favorite ''purified protein'' is as simple as pelleting and re-suspending a sufficient quantity of bacterial cells in an elution buffer.  The speed and simplicity of our IPP system exhibits the utility of synthetic biology for developing new techniques that improve upon established practices.
 
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<div style="text-align:right">'''Continue to [https://2010.igem.org/Team:Washington/Project Project Description &gt;]'''</div>
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Latest revision as of 01:14, 28 October 2010

21stCenturyAntibioticsBanner START button Player 1 Gram(-) Player 2 Gram(+) Gram(+) Side Gram(-) Side


While vital to our quality of life, traditional antibiotics face the serious problems of widespread bacterial resistance and destruction of natural gut flora - problems which call for improved twenty-first century antibiotics. Using synthetic biology tools, we designed, built, and tested two new systems to fight infections by both broad types of bacteria - Gram-positive and Gram-negative. Our first project targets Bacillus anthracis, the Gram-positive pathogen that causes anthrax. We re-engineered an enzyme to remove the pathogen's protective coating, rendering it defenseless against the immune system. In our second project, we re-engineered and transplanted a protein secretion system capable of combating Gram-negative bacteria into E. coli. This system was designed to target Gram-negative pathogens in a modular and controllable fashion. These two systems are the vanguard of a new era of antibiotics using the power of nature harnessed with the tools of synthetic biology.


Gram(-) Therapeutic                  Gram(+) Therapeutic


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