Team:Stockholm/25 July 2010
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The dish had 5 colonies, I inoculated these in 12 ml LB with 24 ul chloramphenocol resistance (50mg/ml). When I know which colony is suitable I will miniprep it and send in to iGEM hq. | The dish had 5 colonies, I inoculated these in 12 ml LB with 24 ul chloramphenocol resistance (50mg/ml). When I know which colony is suitable I will miniprep it and send in to iGEM hq. | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | == Mimmi == | ||
+ | |||
+ | |||
+ | === LMWP === | ||
+ | |||
+ | ==== Re-doing the PCR ==== | ||
+ | |||
+ | |||
+ | * 25 colonies were checked | ||
+ | ** Nina and Johan were running the PCR, I made the gels... | ||
+ | |||
+ | |||
+ | ==== Gels ==== | ||
+ | |||
+ | |||
+ | [[Image:2010-07-25_pSB1C3.LMWP_clone_1-9.jpg|200px|thumb|left]] | ||
+ | {| | ||
+ | ! well | ||
+ | ! sample | ||
+ | |- | ||
+ | | 1 | ||
+ | | ladder | ||
+ | |- | ||
+ | | 2 | ||
+ | | 1control | ||
+ | |- | ||
+ | | 3 | ||
+ | | 3 LMWP C | ||
+ | |- | ||
+ | | 4 | ||
+ | | 4 LMWP C | ||
+ | |- | ||
+ | | 5 | ||
+ | | 5 LMWP C (A) | ||
+ | |- | ||
+ | | 6 | ||
+ | | 6 LMWP C (B) | ||
+ | |- | ||
+ | | 7 | ||
+ | | 7 LMWP C (C) | ||
+ | |- | ||
+ | | 8 | ||
+ | | | ||
+ | |- | ||
+ | | 9 | ||
+ | | 9 LMWP N (C) | ||
+ | |- | ||
+ | | | ||
+ | |} | ||
+ | |||
+ | |||
+ | |||
+ | [[Image:2010-07-25_pSB1C3.LMWP_clone_10-25.jpg|200px|thumb|left]] | ||
+ | {| | ||
+ | ! well | ||
+ | ! sample | ||
+ | ! well | ||
+ | ! sample | ||
+ | |- | ||
+ | | 1 | ||
+ | | ladder | ||
+ | | 12 | ||
+ | | ladder | ||
+ | |- | ||
+ | | 2 | ||
+ | | 10 LMWP N (B) | ||
+ | | 13 | ||
+ | | empty | ||
+ | |- | ||
+ | | 3 | ||
+ | | 11 LMWP N (A) | ||
+ | | 14 | ||
+ | | 18 LMWP N | ||
+ | |- | ||
+ | | 4 | ||
+ | | 12 LMWP N | ||
+ | | 15 | ||
+ | | empty | ||
+ | |- | ||
+ | | 5 | ||
+ | | 13 LMWP N | ||
+ | | 16 | ||
+ | | empty | ||
+ | |- | ||
+ | | 6 | ||
+ | | 14 LMWP N | ||
+ | | 17 | ||
+ | | 19 LMWP N | ||
+ | |- | ||
+ | | 7 | ||
+ | | 15 LMWP N | ||
+ | | 18 | ||
+ | | 20 LMWP N | ||
+ | |- | ||
+ | | 8 | ||
+ | | 16 LMWP N | ||
+ | | 19 | ||
+ | | 21 LMWP N | ||
+ | |- | ||
+ | | 9 | ||
+ | | 17 LMWP N | ||
+ | | 20 | ||
+ | | 22 LMWP N | ||
+ | |- | ||
+ | | 10 | ||
+ | | empty | ||
+ | | 21 | ||
+ | | 23 LMWP N | ||
+ | |- | ||
+ | | 11 | ||
+ | | 25 LMWP N | ||
+ | | 22 | ||
+ | | 24 LMWP N | ||
+ | |} | ||
+ | |||
+ | {{Stockholm/Footer}} |
Latest revision as of 10:52, 26 October 2010
Contents |
Nina
Inoculation of IgG protease
The dish had 5 colonies, I inoculated these in 12 ml LB with 24 ul chloramphenocol resistance (50mg/ml). When I know which colony is suitable I will miniprep it and send in to iGEM hq.
Mimmi
LMWP
Re-doing the PCR
- 25 colonies were checked
- Nina and Johan were running the PCR, I made the gels...
Gels
well | sample |
---|---|
1 | ladder |
2 | 1control |
3 | 3 LMWP C |
4 | 4 LMWP C |
5 | 5 LMWP C (A) |
6 | 6 LMWP C (B) |
7 | 7 LMWP C (C) |
8 | |
9 | 9 LMWP N (C) |
well | sample | well | sample |
---|---|---|---|
1 | ladder | 12 | ladder |
2 | 10 LMWP N (B) | 13 | empty |
3 | 11 LMWP N (A) | 14 | 18 LMWP N |
4 | 12 LMWP N | 15 | empty |
5 | 13 LMWP N | 16 | empty |
6 | 14 LMWP N | 17 | 19 LMWP N |
7 | 15 LMWP N | 18 | 20 LMWP N |
8 | 16 LMWP N | 19 | 21 LMWP N |
9 | 17 LMWP N | 20 | 22 LMWP N |
10 | empty | 21 | 23 LMWP N |
11 | 25 LMWP N | 22 | 24 LMWP N |