Team:Newcastle/25 August 2010

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(New page: =''yneA''= ==Gel Electrophoresis== ===Aim=== To check if the digestion we did yesterday worked. ===Materials and Protocol=== Please refer to [[Team:N...)
(Results and Conclusion)
 
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=''yneA''=
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==Gel Electrophoresis==
 
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===Aim===
 
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To check if the digestion we did [[Team:Newcastle/24_August_2010|yesterday]] worked.
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=Transformation of ''Bacillius subtilis'' 168 with pGFP-rrnB containing ''yneA''=
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===Materials and Protocol===
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==Aim==
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Please refer to [[Team:Newcastle/Gel_electrophoresis|gel electrophoresis]] for protocol.
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We performed gel electrophoresis on our 12 digested minipreps and found 8 were the correct product. We transformed ''B. subtilis'' 168 with one of the samples (1).
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===Results===
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[[Image:filamentous_in_pgfprrnb.jpg|800px]]
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The result from gel electrophoresis:
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==Materials and Protocol==
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Please refer to [[Team:Newcastle/Transformation_of_B._subtilis|transformation of ''B. subtilis'']].
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===Discussion===
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==Results and Conclusion==
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The bands we got from the gel shows that digestion in tubes 1, 2, 4, 10, 11 and 12 worked.
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Please refer to results in [[Team:Newcastle/26_August_2010|tomorrow]]'s lab book.
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==Conclusion===
 
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We use the digestion from the six tubes that worked for ligation.
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{{Team:Newcastle/footer}}

Latest revision as of 02:04, 28 October 2010

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Contents

Transformation of Bacillius subtilis 168 with pGFP-rrnB containing yneA

Aim

We performed gel electrophoresis on our 12 digested minipreps and found 8 were the correct product. We transformed B. subtilis 168 with one of the samples (1).

Filamentous in pgfprrnb.jpg

Materials and Protocol

Please refer to transformation of B. subtilis.

Results and Conclusion

Please refer to results in tomorrow's lab book.


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