Team:Groningen/Growing Streptomyces
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Joelkuiper (Talk | contribs) (New page: -Make plates with a thick layer of medium, 100 ml medium per big Petri dish.Medium of Streptomyces is mannitol soya flour medium (MS or SFM) 300 ml per 1L bottle to avoid boiling over in a...) |
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- | + | '''Concentrations for antibiotics ''' | |
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- | - | + | Antibiotic Stock E.coli B.subtilis |
- | + | *Ampicillin (Amp) (in H2O) 50 mg/ml (4 °C) 100(μg/ml) (:500) | |
- | - | + | *Kanamycin (Km) 10 mg/ml (4 °C) 20 (μg/ml) (:500) 10 (μg/ml) (:100) |
+ | *Spectinomycin (Spec) (in H2O) 10 mg/ml (4 °C) 100(μg/ml) (:100) 100 (μg/ml) (:100) | ||
+ | *Erythromycin (Ery) E.coli stock 10 mg/ml (-18 °C) 150 (μg/ml) (:66.6) | ||
+ | *Erythromycin(Ery) B.subtilis stock 1 mg/ml (-18 °C) 2-5 (μg/ml) | ||
+ | *Tetracyclin (Tet) (50% EtOH) 3 mg/ml (-18 °C) 12 (μg/ml) (:250) 6(μg/ml) (:500) | ||
+ | *Chloramphenicol (Cm) (50% EtOH) 5 mg/ml (-18 °C) 10-15 (μg/ml) (:500/333) 5(μg/ml) (:1000) | ||
+ | *Lyncomycin 12.5 mg/ml (4 °C) 12.5 (μg/ml) (1:1000)* | ||
+ | *Hygromycin 50 mg/ml (4 °C) 125 (μg/ml) (:400) |
Latest revision as of 17:19, 9 September 2010
Concentrations for antibiotics
Antibiotic Stock E.coli B.subtilis
- Ampicillin (Amp) (in H2O) 50 mg/ml (4 °C) 100(μg/ml) (:500)
- Kanamycin (Km) 10 mg/ml (4 °C) 20 (μg/ml) (:500) 10 (μg/ml) (:100)
- Spectinomycin (Spec) (in H2O) 10 mg/ml (4 °C) 100(μg/ml) (:100) 100 (μg/ml) (:100)
- Erythromycin (Ery) E.coli stock 10 mg/ml (-18 °C) 150 (μg/ml) (:66.6)
- Erythromycin(Ery) B.subtilis stock 1 mg/ml (-18 °C) 2-5 (μg/ml)
- Tetracyclin (Tet) (50% EtOH) 3 mg/ml (-18 °C) 12 (μg/ml) (:250) 6(μg/ml) (:500)
- Chloramphenicol (Cm) (50% EtOH) 5 mg/ml (-18 °C) 10-15 (μg/ml) (:500/333) 5(μg/ml) (:1000)
- Lyncomycin 12.5 mg/ml (4 °C) 12.5 (μg/ml) (1:1000)*
- Hygromycin 50 mg/ml (4 °C) 125 (μg/ml) (:400)