Team:Caltech/Parts

From 2010.igem.org

(Difference between revisions)
(Prototype team page)
m
 
(20 intermediate revisions not shown)
Line 1: Line 1:
-
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
+
{{Caltech_iGEM_10|
 +
Content=
 +
<br />
 +
= Favorite Bricks & Characterization Data =
 +
Heat shock promoter: [[BBa_K338001]]
-
<html>
+
HSP-LacI: [[BBa_K338002]]
-
<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
+
-
<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
+
-
This is a template page. READ THESE INSTRUCTIONS.
+
-
</div>
+
-
<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
+
-
You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
+
-
</div>
+
-
<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
+
-
You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
+
-
</div>
+
-
</div>
+
-
</html>
+
-
<!-- *** End of the alert box *** -->
+
Plastic, part 1: [[Team:Caltech/BBa_K338003|BBa_K338003]]
 +
Plastic, part 2: [[Team:Caltech/BBa_K338004|BBa_K338004]]
 +
= All Bricks=
 +
<!-- Following code generated by CompletedPartsFormatter, (c) 2010 Alexandre Boulgakov -->
 +
{{{!}} border="1" cellpadding="5" cellspacing="0"
 +
! scope="col" {{!}} Part Name
 +
! scope="col" {{!}} Brick Number
 +
! scope="col" {{!}} Resistance
 +
! scope="col" {{!}} Subparts
 +
{{!}}-
 +
{{!}} phaB1.RBS (plastic intermediate) {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K332023 BBa_K332023] {{!}}{{!}} Cm {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K156013 BBa_K156013], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
 +
{{!}}-
 +
{{!}} Heat-Shock Activated Promoter {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338001 BBa_K338001] {{!}}{{!}} Amp {{!}}{{!}} Original part
 +
{{!}}-
 +
{{!}} Heat-Shock or IPTG Induced Generator {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338002 BBa_K338002] {{!}}{{!}} Kan {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338001 BBa_K338001], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0011 BBa_R0011], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
 +
{{!}}-
 +
{{!}} pLacI.RBS.phaA.RBS (plastic intermediate) {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338003 BBa_K338003] {{!}}{{!}} Kan {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0011 BBa_R0011], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K156012 BBa_K156012], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
 +
{{!}}-
 +
{{!}} phaB1.RBS.phaC1.TT (plastic intermediate) {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338004 BBa_K338004] {{!}}{{!}} Kan {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K156013 BBa_K156013], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K156014 BBa_K156014], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
 +
{{!}}-
 +
{{!}} phaA.RBS (plastic intermediate) {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338022 BBa_K338022] {{!}}{{!}} Cm {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K156012 BBa_K156012], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
 +
{{!}}-
 +
{{!}} phaC1 with Double Terminator {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338024 BBa_K338024] {{!}}{{!}} Cm {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K156014 BBa_K156014], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
 +
{{!}}-
 +
{{!}} +OmpR, +(CinR-HSL) Double Promoter {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338029 BBa_K338029] {{!}}{{!}} Tet {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0082 BBa_R0082], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0077 BBa_R0077]
 +
{{!}}-
 +
{{!}} GFP Reporter Device, Heat-Shock Induced {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338042 BBa_K338042] {{!}}{{!}} Kan {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338001 BBa_K338001], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0040 BBa_E0040], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
 +
{{!}}-
 +
{{!}} +Heat-Shock, +CinR-HSL Double Promoter {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338046 BBa_K338046] {{!}}{{!}} Kan {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338001 BBa_K338001], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0077 BBa_R0077]
 +
{{!}}-
 +
{{!}} GFP Reporter Device, Heat-Shock or IPTG Induced {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338062 BBa_K338062] {{!}}{{!}} Cm {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338001 BBa_K338001], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0011 BBa_R0011], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0040 BBa_E0040], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
 +
{{!}}-
 +
{{!}} Heat-Shock Activated Generator {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338081 BBa_K338081] {{!}}{{!}} Kan {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338001 BBa_K338001], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
 +
{{!}}-
 +
{{!}} GFP Reporter Device, Cinr-HSL Induced {{!}}{{!}} Not Submitted {{!}}{{!}} Cm {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0077 BBa_R0077], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0040 BBa_E0040], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
 +
{{!}}-
 +
{{!}} HSL with Double Terminator {{!}}{{!}} Not Submitted {{!}}{{!}} Cm {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0076 BBa_C0076], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
 +
{{!}}-
 +
{{!}} Constitutive Generator {{!}}{{!}} Not Submitted {{!}}{{!}} Kan {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23119 BBa_J23119], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
 +
{{!}}-
 +
{{!}} CinR with Double Terminator {{!}}{{!}} Not Submitted {{!}}{{!}} Cm {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0077 BBa_C0077], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
 +
{{!}}-
 +
{{!}} +IPTG, +(CinR-HSL) Double Promoter {{!}}{{!}} Not Submitted {{!}}{{!}} Cm {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0011 BBa_R0011], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0077 BBa_R0077]
 +
{{!}}-
 +
{{!}} OmpR Induced Generator {{!}}{{!}} Not Submitted {{!}}{{!}} Tet {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0082 BBa_R0082], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
 +
{{!}}-
 +
{{!}} HSL.TT.pLacI.p(CinR-HSL) (intermediate) {{!}}{{!}} Not Submitted {{!}}{{!}} Amp {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0076 BBa_C0076], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0011 BBa_R0011], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0077 BBa_R0077]
 +
{{!}}-
 +
{{!}} HSL Device, CinR-HSL Induced {{!}}{{!}} Not Submitted {{!}}{{!}} Kan {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0077 BBa_R0077], [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0076 BBa_C0076], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
 +
{{!}}-
 +
{{!}} HSL Device {{!}}{{!}} Not Submitted {{!}}{{!}} Kan {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0076 BBa_C0076], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
 +
{{!}}-
 +
{{!}} Heat-Shock Activated Generator {{!}}{{!}} Not Submitted {{!}}{{!}} Kan {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338001 BBa_K338001], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
 +
{{!}}-
 +
{{!}} GFP Reporter Device, OmpR Induced {{!}}{{!}} Not Submitted {{!}}{{!}} Cm {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0082 BBa_R0082], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0040 BBa_E0040], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
 +
{{!}}-
 +
{{!}} GFP Reporter Device, Constitutive {{!}}{{!}} Not Submitted {{!}}{{!}} Kan {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23119 BBa_J23119], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0040 BBa_E0040], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
 +
{{!}}-
 +
{{!}} K124014 Holin with Double Terminator {{!}}{{!}} Not Submitted {{!}}{{!}} Cm {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K124014 BBa_K124014], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
 +
{{!}}-
 +
{{!}} K124017 Lysis Cassette with Double Terminator {{!}}{{!}} Not Submitted {{!}}{{!}} Cm {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K124017 BBa_K124017], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
 +
{{!}}-
 +
{{!}} CinR Device, OmpR or CinR-HSL Induced {{!}}{{!}} Not Submitted {{!}}{{!}} Amp {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0082 BBa_R0082], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0077 BBa_R0077], [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0077 BBa_C0077], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
 +
{{!}}-
 +
{{!}} CinR Device, OmpR Induced {{!}}{{!}} Not Submitted {{!}}{{!}} Amp {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0082 BBa_R0082], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0077 BBa_C0077], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
 +
{{!}}}
 +
<!-- End CompletedPartsFormatter section. -->
-
{|align="justify"
+
If your iGEM team is interested in any of our unsubmitted parts, let us know and we might be able to send them to you directly.  
-
|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
+
-
|[[Image:Caltech_logo.png|200px|right|frame]]
+
-
|-
+
-
|
+
-
''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
+
-
|[[Image:Caltech_team.png|right|frame|Your team picture]]
+
-
|-
+
-
|
+
-
|align="center"|[[Team:Caltech | Team Example]]
+
-
|}
+
-
<!--- The Mission, Experiments --->
+
Email: igem at caltech dot edu
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
= Apparatus =
-
!align="center"|[[Team:Caltech|Home]]
+
[[Image:Caltech2010Lamp.JPG|thumb|400px|The red/infrared light apparatus]]
-
!align="center"|[[Team:Caltech/Team|Team]]
+
-
!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=Caltech Official Team Profile]
+
-
!align="center"|[[Team:Caltech/Project|Project]]
+
-
!align="center"|[[Team:Caltech/Parts|Parts Submitted to the Registry]]
+
-
!align="center"|[[Team:Caltech/Modeling|Modeling]]
+
-
!align="center"|[[Team:Caltech/Notebook|Notebook]]
+
-
!align="center"|[[Team:Caltech/Safety|Safety]]
+
-
|}
+
 +
The red/infrared light emitting apparatus we built to test our heat shock promoter was modeled after the original [http://openwetware.org/wiki/LightCannon Light Cannon]. It features a theater lamp with a Roscolux #22 Deep Amber red/infrared light filter ([http://www.amazon.com/Rosco-Laboratories-22-ROSCO-Roscolux-22/dp/B000XRCGDE/ref=sr_1_1?ie=UTF8&s=electronics&qid=1287956720&sr=8-1 Amazon], [http://www.rosco.com/includes/filters/SED.asp?titleName=R22:%20Deep%20Amber&imageName=../../images/filters/roscolux/22.jpg Spectrum Data]).
 +
We placed this light on the bench top above a rudimentary stage. By varying the height of the lamp above the stage, we could vary the temperature and light intensity at the stage. In the image, the stage temperature was measured to be 42&deg;C.
-
===Parts===
+
[[Image:Caltech2010Stage.JPG|thumb|left|350px|Stage of the light apparatus; temperature at 42&deg;C.]]
-
New for iGEM 2010 is the ''groupparts'' tag.  This tag will generate a table with all of the parts that your team adds to your team sandbox.  Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.
+
&nbsp;
-
 
+
}}
-
<groupparts>iGEM010 Caltech</groupparts>
+

Latest revision as of 22:01, 27 October 2010


iGEM 2010



Home

People

Project Details

Protocols

Completed Systems

Notebook

Biosafety

Human Impact

References

Support


Caltech logo watermark.png

Favorite Bricks & Characterization Data

Heat shock promoter: BBa_K338001

HSP-LacI: BBa_K338002

Plastic, part 1: BBa_K338003

Plastic, part 2: BBa_K338004

All Bricks

Part Name Brick Number Resistance Subparts
phaB1.RBS (plastic intermediate) [http://partsregistry.org/wiki/index.php?title=Part:BBa_K332023 BBa_K332023] Cm [http://partsregistry.org/wiki/index.php?title=Part:BBa_K156013 BBa_K156013], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
Heat-Shock Activated Promoter [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338001 BBa_K338001] Amp Original part
Heat-Shock or IPTG Induced Generator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338002 BBa_K338002] Kan [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338001 BBa_K338001], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0011 BBa_R0011], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
pLacI.RBS.phaA.RBS (plastic intermediate) [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338003 BBa_K338003] Kan [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0011 BBa_R0011], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K156012 BBa_K156012], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
phaB1.RBS.phaC1.TT (plastic intermediate) [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338004 BBa_K338004] Kan [http://partsregistry.org/wiki/index.php?title=Part:BBa_K156013 BBa_K156013], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K156014 BBa_K156014], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
phaA.RBS (plastic intermediate) [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338022 BBa_K338022] Cm [http://partsregistry.org/wiki/index.php?title=Part:BBa_K156012 BBa_K156012], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
phaC1 with Double Terminator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338024 BBa_K338024] Cm [http://partsregistry.org/wiki/index.php?title=Part:BBa_K156014 BBa_K156014], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
+OmpR, +(CinR-HSL) Double Promoter [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338029 BBa_K338029] Tet [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0082 BBa_R0082], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0077 BBa_R0077]
GFP Reporter Device, Heat-Shock Induced [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338042 BBa_K338042] Kan [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338001 BBa_K338001], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0040 BBa_E0040], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
+Heat-Shock, +CinR-HSL Double Promoter [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338046 BBa_K338046] Kan [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338001 BBa_K338001], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0077 BBa_R0077]
GFP Reporter Device, Heat-Shock or IPTG Induced [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338062 BBa_K338062] Cm [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338001 BBa_K338001], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0011 BBa_R0011], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0040 BBa_E0040], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
Heat-Shock Activated Generator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338081 BBa_K338081] Kan [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338001 BBa_K338001], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
GFP Reporter Device, Cinr-HSL Induced Not Submitted Cm [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0077 BBa_R0077], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0040 BBa_E0040], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
HSL with Double Terminator Not Submitted Cm [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0076 BBa_C0076], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
Constitutive Generator Not Submitted Kan [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23119 BBa_J23119], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
CinR with Double Terminator Not Submitted Cm [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0077 BBa_C0077], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
+IPTG, +(CinR-HSL) Double Promoter Not Submitted Cm [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0011 BBa_R0011], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0077 BBa_R0077]
OmpR Induced Generator Not Submitted Tet [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0082 BBa_R0082], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
HSL.TT.pLacI.p(CinR-HSL) (intermediate) Not Submitted Amp [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0076 BBa_C0076], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0011 BBa_R0011], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0077 BBa_R0077]
HSL Device, CinR-HSL Induced Not Submitted Kan [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0077 BBa_R0077], [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0076 BBa_C0076], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
HSL Device Not Submitted Kan [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0076 BBa_C0076], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
Heat-Shock Activated Generator Not Submitted Kan [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338001 BBa_K338001], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
GFP Reporter Device, OmpR Induced Not Submitted Cm [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0082 BBa_R0082], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0040 BBa_E0040], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
GFP Reporter Device, Constitutive Not Submitted Kan [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23119 BBa_J23119], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0040 BBa_E0040], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
K124014 Holin with Double Terminator Not Submitted Cm [http://partsregistry.org/wiki/index.php?title=Part:BBa_K124014 BBa_K124014], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
K124017 Lysis Cassette with Double Terminator Not Submitted Cm [http://partsregistry.org/wiki/index.php?title=Part:BBa_K124017 BBa_K124017], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
CinR Device, OmpR or CinR-HSL Induced Not Submitted Amp [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0082 BBa_R0082], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0077 BBa_R0077], [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0077 BBa_C0077], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
CinR Device, OmpR Induced Not Submitted Amp [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0082 BBa_R0082], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0077 BBa_C0077], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]

If your iGEM team is interested in any of our unsubmitted parts, let us know and we might be able to send them to you directly.

Email: igem at caltech dot edu

Apparatus

The red/infrared light apparatus

The red/infrared light emitting apparatus we built to test our heat shock promoter was modeled after the original [http://openwetware.org/wiki/LightCannon Light Cannon]. It features a theater lamp with a Roscolux #22 Deep Amber red/infrared light filter ([http://www.amazon.com/Rosco-Laboratories-22-ROSCO-Roscolux-22/dp/B000XRCGDE/ref=sr_1_1?ie=UTF8&s=electronics&qid=1287956720&sr=8-1 Amazon], [http://www.rosco.com/includes/filters/SED.asp?titleName=R22:%20Deep%20Amber&imageName=../../images/filters/roscolux/22.jpg Spectrum Data]).

We placed this light on the bench top above a rudimentary stage. By varying the height of the lamp above the stage, we could vary the temperature and light intensity at the stage. In the image, the stage temperature was measured to be 42°C.

Stage of the light apparatus; temperature at 42°C.
 
Error creating thumbnail: /websites/2010.igem.org/wiki/bin/ulimit4.sh: line 4: convert: command not found
Caltech footer.png


Locations of visitors to this page

Retrieved from "http://2010.igem.org/Team:Caltech/Parts"