Team:Newcastle/20 August 2010

From 2010.igem.org

(Difference between revisions)
(→PCR of pSB1C3 for rocF and Subtilin Immunity)
 
(75 intermediate revisions not shown)
Line 1: Line 1:
{{Team:Newcastle/mainbanner}}
{{Team:Newcastle/mainbanner}}
-
=Subtilin Immunity=
+
=Transformation of ligated ''yneA'' into pGFPrrnB and pSB1C3=
-
 
+
-
==
+
-
 
+
-
=PCR of pSB1C3 for rocF and Subtilin Immunity=
+
==Aims==
==Aims==
-
Following yesterday's Phusion PCR, we plan to first perform gel electrophoresis, then gel extraction, and finally nanodrop to record the level of DNA.
+
To produce more colonies of the plasmid pGFPrrnB and pSB1C3 containing ''yneA'' with pGFPrrnB and pSB1C3 from[[Team:Newcastle/19_August_2010|yesterday]].
-
 
+
-
==Materials and protocol==
+
-
 
+
-
Please refer to the [[Team:Newcastle/Gel_electrophoresis| gel electrophoresis]], [[Team:Newcastle/Gel_extraction| gel extraction]] and [[TeamNewcastleNanoDrop_Spectrophotometer| NanoDrop]] protocols. '''Vacuum manifold''' will be used.
+
-
 
+
-
=Gel Extraction=
+
-
 
+
-
==Aims==
+
-
 
+
-
To purify the PCR products of digested ''yneA'', pGFPrrnB and pSB1C3 from [[Team:Newcastle/19_August_2010|yesterday]].
+
-
 
+
-
==Materials and Protocol==
+
-
 
+
-
Please refer to [[Team:Newcastle/Gel_extraction|gel extraction]] and [[TeamNewcastleNanoDrop_Spectrophotometer|nanodrop]].
+
-
 
+
-
==Results==
+
-
 
+
-
==Discussion==
+
-
 
+
-
==Conclusion==
+
-
 
+
-
 
+
-
=Miniprep of ''yneA'', pGFPrrnB and pSB1C3=
+
-
 
+
-
==Aim==
+
-
 
+
-
To produce stocks of ''yneA'', pGFPrrnB and pSB1C3.
+
-
 
+
-
==Materials and Protocol==
+
-
 
+
-
Please refer to [[Team:Newcastle/Qiagen_Minipreps|miniprep]] and [[TeamNewcastleNanoDrop_Spectrophotometer|nanodrop]].
+
-
 
+
-
==Results==
+
-
 
+
-
==Discussion==
+
-
 
+
-
==Conclusion==
+
-
 
+
-
 
+
-
=Transformation of Ligated Products=
+
-
 
+
-
==Aims==
+
-
 
+
-
To produce more colonies of the ligated products of ''yneA'' with pGFPrrnB and pSB1C3.
+
==Materials and Protocol==
==Materials and Protocol==
Please refer to [[Team:Newcastle/Transformation_of_E._coli|transformation of E. coli]].
Please refer to [[Team:Newcastle/Transformation_of_E._coli|transformation of E. coli]].
-
 
-
 
-
=Ligation of ''yneA'' with Vectors=
 
-
 
-
==Aims==
 
-
 
-
To ligate ''yneA'' with pGFPrrnB and ''yneA'' with pSB1C3 (A repeat of [[Team:Newcastle/19_August_2010|yesterday]].
 
-
 
-
==Materials and Protocol==
 
-
 
-
Please refer to [[Team:Newcastle/Ligation|ligation]].
 
-
 
-
==Results, Discussion and Conclusion==
 
-
 
-
Please refer to [[Team:Newcastle/23_August_2010|23.08.10]].
 
-
 
-
 
-
 
-
 
-
 
{{Team:Newcastle/footer}}
{{Team:Newcastle/footer}}

Latest revision as of 00:33, 28 October 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Transformation of ligated yneA into pGFPrrnB and pSB1C3

Aims

To produce more colonies of the plasmid pGFPrrnB and pSB1C3 containing yneA with pGFPrrnB and pSB1C3 fromyesterday.

Materials and Protocol

Please refer to transformation of E. coli.


Newcastle University logo.png    Newcastle cbcb logo.pngNewcastle Biomedicine logo.gif    Team Newcastle CEG logo.gif
Newcastle iww logo.jpg  UNIPV Pavia Logo.gif  Newcastle BBSRC.gif    Newcastle Genevision logo.png Newcastle WelcomeTrust.jpg
FaceBook Icon
Retrieved from "http://2010.igem.org/Team:Newcastle/20_August_2010"