Team:Newcastle/20 August 2010

From 2010.igem.org

(Difference between revisions)
 
(80 intermediate revisions not shown)
Line 1: Line 1:
{{Team:Newcastle/mainbanner}}
{{Team:Newcastle/mainbanner}}
-
=Aims=
+
=Transformation of ligated ''yneA'' into pGFPrrnB and pSB1C3=
-
Following yesterday's Phusion PCR, we plan to first perform gel electrophoresis, then gel extraction, and finally nanodrop to record the level of DNA.
+
==Aims==
-
==Materials and protocol==
+
To produce more colonies of the plasmid pGFPrrnB and pSB1C3 containing ''yneA'' with pGFPrrnB and pSB1C3 from[[Team:Newcastle/19_August_2010|yesterday]].
-
 
+
-
Please refer to the [[Team:Newcastle/Gel_electrophoresis| gel electrophoresis]], [[Team:Newcastle/Gel_extraction| gel extraction]] and [[TeamNewcastleNanoDrop_Spectrophotometer| NanoDrop]] protocols. '''Vacuum manifold''' will be used.
+
-
 
+
-
 
+
-
=Gel Extraction=
+
-
 
+
-
==Aims==
+
-
 
+
-
To purify the PCR products of digested ''yneA'', pGFPrrnB and pSB1C3 from [[Team:Newcastle/19_August_2010|yesterday]].
+
==Materials and Protocol==
==Materials and Protocol==
-
Please refer to [[Team:Newcastle/Gel_extraction|gel extraction]].
+
Please refer to [[Team:Newcastle/Transformation_of_E._coli|transformation of E. coli]].
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
{{Team:Newcastle/footer}}
{{Team:Newcastle/footer}}

Latest revision as of 00:33, 28 October 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Transformation of ligated yneA into pGFPrrnB and pSB1C3

Aims

To produce more colonies of the plasmid pGFPrrnB and pSB1C3 containing yneA with pGFPrrnB and pSB1C3 fromyesterday.

Materials and Protocol

Please refer to transformation of E. coli.


Newcastle University logo.png    Newcastle cbcb logo.pngNewcastle Biomedicine logo.gif    Team Newcastle CEG logo.gif
Newcastle iww logo.jpg  UNIPV Pavia Logo.gif  Newcastle BBSRC.gif    Newcastle Genevision logo.png Newcastle WelcomeTrust.jpg
FaceBook Icon