Team:Alberta/project

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[[Image:Alberta Plate highschool.jpg|center|frame|Bryce Stewart, a grade 10 student shows off colonies transformed with a plasmid constructed using the GENOMIKON kit.]]
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==Project Overview==
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''GENOMIKON: an Educational Toolkit for Rapid Genetic Construction''
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Synthetic biology and gene manipulation have potential applications in many fields. Unfortunately, courses in this field are only taught to university students who have already chosen to specialize in genetics. Recognizing that many of the basic principles and methods are easily explained, the University of Alberta sought to make synthetic biology more accessible by creating GENOMIKON, a gene manipulation and synthetic biology kit designed to work in a high school environment. To construct the kit, we first created the BioBytes 2.0 assembly method so that the DNA in the kit could be ligated into plasmids quickly and efficiently. We then designed experiments for the kit with the aim of making them quick, reliable, and easy to perform even with minimal lab equipment.  To make GENOMIKON useful as a classroom resource, an interactive lab manual was created to guide students through their experiments. We also developed a distribution plan for getting the kit into high schools across Canada.  GENOMIKON's aim is to be a fun way of giving students an introduction to genetics and molecular biology, even before they enter post-secondary education.  From our experience over the past six months, we believe it will be a success.
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==[[Team:Alberta/biobyte2 |BioBytes Theory]]==
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#highlight
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The DNA components of the GENOMIKON kit will be provided in linear pieces called BioBytes.  These Bytes have 5' overhangs, four bases long on both ends, which gives specificity to the Bytes that can neighbor them. BioBytes come in two formats: 'AB Bytes' and 'BA Bytes'. AB Bytes can only be ligated to BA Bytes and vice versa. This leads to a construct alternating in AB and BA Bytes. The bytes are bound to an anchor which specifies the order of alternation between AB and BA bytes.
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==[[Team:Alberta/Kit |The GENOMIKON Kit]]==
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The GENOMIKON kit was designed for use in a high school environment, however most high schools lack the lab equipment traditionally used to perform genetic experiments.  Fortunately, the BioBytes 2.0 assembly method does not require centrifuges, thermocyclers or extreme accuracy with regards to volume and temperature. The only equipment a high school must supply is a hot plate and a beaker.  Creating a sterile environment in a high school lab was another challenge that had to be addressed.  The reagents in the kit are sent in individual sterilized packages along with everything that a high school would need to perform the transformations using synthetic parts.
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==[[Team:Alberta/Software |GENOMIKON ONLINE]]==
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GENOMIKON.ca was created as a lab manual to accompany the GENOMIKON kit.  It was designed to act as a teaching resource to give students background information for the experiments they design and to introduce them to the kit and its uses. Features include BioByte descriptions, an interactive plasmid construction tool where students can engineer their own plasmids by dragging and dropping different parts into sequence, a glossary and encyclopedia. Lastly, the teacher can coordinate the class experiments by working through the groups feature.
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genomikon</div>
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<div id="navbar"><span class="llink2"><a href="https://2010.igem.org/Team:Alberta/JTest"> home</a> |  <a href="project"> project</a> | <a href="human practices"> human practices</a> | <a href="parts"> parts</a> | <a href="software"> software</a> | <a href="https://2010.igem.org/Team:Alberta/Notebook"> notebook</a> | <a href="team"> team</a></span>
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<p><i>Genomikon: an Educational Toolkit for the Rapid Assembly of Plasmids</i></p>
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<p>Synthetic biology is a new and expanding field that has potential applications in every aspect of society. Today, synthetic biology is underused because it is expensive and slow. The University of Alberta is developing a method of plasmid assembly that is modular, rapid, and inexpensive. We are in process of designing an educational kit, Genomikon, which will enable anyone to construct a plasmid without special equipment. We will also provide a comprehensive lab manual to accomodate those new to the field.</p>
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<p>To demonstrate the accessibility of our Genomikon toolkit, we will implement it in a high school laboratory setting. Genetic parts will be adapted into a system called BioBytes. This system will allow students to rationally create functional plasmids. The kit’s flexiblity will accomodate both predesigned experiments and new creative expressions. We believe that our kit could revolutionize science education. Our project not only seeks to optimize current procedures of synthetic biology but will also expose a new generation of students to this exciting field.</p>
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<p>The DNA components of the Genomikon kit will be provided in linear pieces named BioBytes.  These Bytes have 4 base 5' overhangs on both ends which gives specificity to the bytes that can neighbor it. BioBytes come in two flavors: A bytes and B bytes, which are defined by the composition of the overhangs it has. The result is that A bytes can only be ligated to B bytes and vise versa leading to a construct alternating in A and B bytes.  The specificity in absolute order of a construct is given by an anchor which restricts ligation to only one side.  </p>
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<a href="Site_Map">site map</a>|<a href="Sponsors">sponsors</a>|<a href="Contact">contact us</a>
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Latest revision as of 03:57, 28 October 2010

TEAM ALBERTA

Project Overview

GENOMIKON: an Educational Toolkit for Rapid Genetic Construction

Synthetic biology and gene manipulation have potential applications in many fields. Unfortunately, courses in this field are only taught to university students who have already chosen to specialize in genetics. Recognizing that many of the basic principles and methods are easily explained, the University of Alberta sought to make synthetic biology more accessible by creating GENOMIKON, a gene manipulation and synthetic biology kit designed to work in a high school environment. To construct the kit, we first created the BioBytes 2.0 assembly method so that the DNA in the kit could be ligated into plasmids quickly and efficiently. We then designed experiments for the kit with the aim of making them quick, reliable, and easy to perform even with minimal lab equipment. To make GENOMIKON useful as a classroom resource, an interactive lab manual was created to guide students through their experiments. We also developed a distribution plan for getting the kit into high schools across Canada. GENOMIKON's aim is to be a fun way of giving students an introduction to genetics and molecular biology, even before they enter post-secondary education. From our experience over the past six months, we believe it will be a success.

BioBytes Theory

The DNA components of the GENOMIKON kit will be provided in linear pieces called BioBytes. These Bytes have 5' overhangs, four bases long on both ends, which gives specificity to the Bytes that can neighbor them. BioBytes come in two formats: 'AB Bytes' and 'BA Bytes'. AB Bytes can only be ligated to BA Bytes and vice versa. This leads to a construct alternating in AB and BA Bytes. The bytes are bound to an anchor which specifies the order of alternation between AB and BA bytes.

The GENOMIKON Kit

The GENOMIKON kit was designed for use in a high school environment, however most high schools lack the lab equipment traditionally used to perform genetic experiments. Fortunately, the BioBytes 2.0 assembly method does not require centrifuges, thermocyclers or extreme accuracy with regards to volume and temperature. The only equipment a high school must supply is a hot plate and a beaker. Creating a sterile environment in a high school lab was another challenge that had to be addressed. The reagents in the kit are sent in individual sterilized packages along with everything that a high school would need to perform the transformations using synthetic parts.

GENOMIKON ONLINE

GENOMIKON.ca was created as a lab manual to accompany the GENOMIKON kit. It was designed to act as a teaching resource to give students background information for the experiments they design and to introduce them to the kit and its uses. Features include BioByte descriptions, an interactive plasmid construction tool where students can engineer their own plasmids by dragging and dropping different parts into sequence, a glossary and encyclopedia. Lastly, the teacher can coordinate the class experiments by working through the groups feature.


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