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UTDallas/16 August 2010
From 2010.igem.org
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===August 16, 2010 === | ===August 16, 2010 === | ||
| - | We started the cloning procedure. We digested some of the parts and then gel purified them. All but two worked. We incubated some of the glycerol stock of the two that did not work. | + | *We started the cloning procedure. |
| + | *We digested some of the parts and then gel purified them. All but two worked. | ||
| + | *We incubated some of the glycerol stock of the two that did not work. | ||
| + | |||
| + | Image 1: There is a 2-log DNA ladder, then there is not a correct band for Pu, then there is a band for PyeaR | ||
| + | [[Image:8-16.JPG |500px | center ]] | ||
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| + | Image 2: There is a 2-log DNA ladder, then Pr+XylR correct, then Terminator incorrect, then Red correct, then GFP correct, then Orange correct, then mRFP1 correct, and then a 1 kb DNA ladder | ||
| + | [[Image:8-16 (2).JPG |500px | center ]] | ||
Latest revision as of 01:33, 2 September 2010
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August 16, 2010
- We started the cloning procedure.
- We digested some of the parts and then gel purified them. All but two worked.
- We incubated some of the glycerol stock of the two that did not work.
Image 1: There is a 2-log DNA ladder, then there is not a correct band for Pu, then there is a band for PyeaR
Image 2: There is a 2-log DNA ladder, then Pr+XylR correct, then Terminator incorrect, then Red correct, then GFP correct, then Orange correct, then mRFP1 correct, and then a 1 kb DNA ladder
.JPG)
