Team:MIT

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<h2 class="ui-widget-header ui-corner-all">Project Description</h2>
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In an effort to form self-assembling three-dimensional biomaterials, we are attempting two parallel paths in mammalian differentiation and phage polymerization. In mammalian cells, our goal is to induce bone formation in response to a pressure stimulus. Our system will sense mechanical stimulation via a pressure-sensitive promoter, identified by screening the fluid shear stress response of a library of candidate promoters in microfluidic devices. The activity of this promoter will feed into a bi-stable toggle circuit, optimized using a mathematical model, which will convert a transient stimulus into constitutive expression of a factor that induces bone formation. For our phage portion, we plan to induce polymer formation in bacterial cells, using UV light to control the pattern of polymer growth. In addition, we will integrate various fluorescent proteins into our system, each of which is dependent upon quorum sensing processes. Our completed project will allow for the creation of visible bacteriophage structures against a user-directed, multichromatic background.
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<a href="https://static.igem.org/mediawiki/2010/d/dc/Fromabove.JPG" class="thickbox" title="The team. Not pictured, Crystal McKenzie, Arvind Thiagarajan, Lauren McGough, Jason Stevens."><img src="https://static.igem.org/mediawiki/2010/d/dc/Fromabove.JPG" width=100%></a>The 2010 MIT iGEM team. We are biological engineers, physicists, electrical engineers, chemical engineers, mathematicians, and computer scientists.
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<table width=650px style="background-color: white; height: 700 px; margin-top:5px; padding: 10px;"><tr><td colspan="3"><div class="bodybaby">Programmable, Self-constructing Biomaterials</div></td>
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<tr><td colspan="3"><br>The 2010 MIT iGEM team focused on the control and production of self-constructing and self-repairing living biomaterials through both bacterial and <div style="display:inline;"><a href="https://static.igem.org/mediawiki/2010/0/0b/Screen_shot_2010-10-24_at_10.25.02_AM.png" class="thickbox" title="Pretty materials. Ours are programmable."><img style="float: right; padding: 10px" src="https://static.igem.org/mediawiki/2010/0/0b/Screen_shot_2010-10-24_at_10.25.02_AM.png" height=147px></a></div> mammalian engineering. We ventured to set up the framework for material formation in both types of cells, for future applications in living, self-repairing materials and in vitro organogenesis respectively.
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We have accomplished far beyond what we expected of ourselves! In addition to our project, we have created a new Mammalian Biobrick standard, contributed original parts for mammalian cells and bacteriophage, and we have biobricked two working toggles for the registry.
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<li style="margin: 3px; padding: 5px; display: inline-block; width:30%; height: 100px; background-color: #8b0000; opacity: 0.75;"><a style="color: white;" href="https://2010.igem.org/Team:MIT_results"><b style="font-size: large;">Results</b><br>Click to see our results in both bacterial and mammalian cells!</a></li>
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<li style="display: inline-block; padding: 5px; margin: 3px; width:30%; height: 100px; background-color: #e6881a;opacity: 0.75;"><a  style="color: white;" href="https://2010.igem.org/Team:MIT_tmodel"><b style="font-size: large;">Modelling</b><br>We created mathematical models of both our bacterial and our mammalian results.</a></li>
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<li style="margin: 3px; display: inline-block; padding: 5px; width:30%; height: 100px; background-color: #016b9d; opacity:0.75;"><a  style="color: white;" href="https://2010.igem.org/Team:MIT_parts"><b style="font-size: large;">Parts</b><br>We added many original parts to the registry. Check them out!</a></li>
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Latest revision as of 01:14, 28 October 2010

MIT iGEM 2010

The 2010 MIT iGEM team. We are biological engineers, physicists, electrical engineers, chemical engineers, mathematicians, and computer scientists.
Programmable, Self-constructing Biomaterials

The 2010 MIT iGEM team focused on the control and production of self-constructing and self-repairing living biomaterials through both bacterial and
mammalian engineering. We ventured to set up the framework for material formation in both types of cells, for future applications in living, self-repairing materials and in vitro organogenesis respectively.

We have accomplished far beyond what we expected of ourselves! In addition to our project, we have created a new Mammalian Biobrick standard, contributed original parts for mammalian cells and bacteriophage, and we have biobricked two working toggles for the registry.

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