Latest revision as of 23:03, 27 October 2010

Subtilin immunity part

Today we were working on our subtilin immunity part cloning strategy.

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-=Transformation=
+=Subtilin immunity part=
-==Results/Aims==
+Today we were working on our subtilin immunity part cloning strategy.
-*pVeg transformed successfully- From this we will set up an overnight broth culture for plasmid extraction
-*''lacI''- To show that we have sucessfully transformed from ''lacI'' we will select 12 colonies and plate them on a section of an agar plate as well as setting up an overnight broth culture.
-=Transformation from ligation products=
-==Aims==
-To transform some competent ''E.coli'' DH5α with the ligation products: 1:3, 1:5 and the vector from the ligation on [[Team:Newcastle/6_August_2010|06.08.10]].
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