Team:Newcastle/15 July 2010

From 2010.igem.org

(Difference between revisions)
 
(9 intermediate revisions not shown)
Line 1: Line 1:
{{Team:Newcastle/mainbanner}}
{{Team:Newcastle/mainbanner}}
 +
=Research=
-
=LacI BioBrick Construction=
+
We initiated our research on subtilin quorum sensing system which would provide a coordinated cell response.
-
==Aims==
+
#Klein C, Kaletta C and Entian KD. (1993). "Biosynthesis of the lantibiotic subtilin is regulated by a histidine kinase/response regulator system". ''Applied and environmental microbiology''. 59(1). 296-303.  
-
*To use PCR to extract ''lacI'' (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP).
+
#Heinzmann S, Entian KD and Stein T. (2006). "Engineering Bacillus subtilis ATCC 6633 for improved production of the lantibiotic subtilin". ''Applied microbiology and biotechnology''. 69(5). 532-6.
-
*The aim of today's experiment is to '''screen for the lacI insert'''. The experiments that were carried out [[Team:Newcastle/16_July_2010#Protocol|yesterday]] are being repeated today as there were extra, unexpected bandings.
+
-
 
+
-
==Protocol==
+
-
#Miniprep - the [[Team:Newcastle/Qiagen_Minipreps|miniprep]] protocol was followed.  
+
-
#Restriction digests - the [[Team:Newcastle/Restriction_digests|restriction digests]] protocol was followed to allow the length of the DNA to be checked using single and double digest.  
+
-
#*Single digest with Pst1
+
-
#*double digests with Pst1 and Xba1
+
-
#Gel electrophoresis - the [[Team:Newcastle/Gel_electrophoresis|gel electrophoresis]] protocol was followed.  
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
Set up liquid broth culture in 5 ml of LB - 1 colony from colonies 1-7
+
{{Team:Newcastle/footer}}
{{Team:Newcastle/footer}}

Latest revision as of 20:19, 25 October 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Research

We initiated our research on subtilin quorum sensing system which would provide a coordinated cell response.

  1. Klein C, Kaletta C and Entian KD. (1993). "Biosynthesis of the lantibiotic subtilin is regulated by a histidine kinase/response regulator system". Applied and environmental microbiology. 59(1). 296-303.
  2. Heinzmann S, Entian KD and Stein T. (2006). "Engineering Bacillus subtilis ATCC 6633 for improved production of the lantibiotic subtilin". Applied microbiology and biotechnology. 69(5). 532-6.
Newcastle University logo.png    Newcastle cbcb logo.pngNewcastle Biomedicine logo.gif    Team Newcastle CEG logo.gif
Newcastle iww logo.jpg  UNIPV Pavia Logo.gif  Newcastle BBSRC.gif    Newcastle Genevision logo.png Newcastle WelcomeTrust.jpg
FaceBook Icon
Retrieved from "http://2010.igem.org/Team:Newcastle/15_July_2010"