"

From 2010.igem.org

Revision as of 14:38, 5 August 2010 (view source) Erik (Talk | contribs) ← Older edit		Latest revision as of 15:52, 5 August 2010 (view source) Anisha (Talk | contribs)
Line 1:		Line 1:
		+	==July 19th==
		+
		+
		+	*Colony PCR:
		+	-J24679+B1002+K3
		+	-J24679+B1006+K3
		+	-Lux+RBS+B1006+K3
		+	-Lux+RBS+B1002+K3
		+	(The extension time used was a minute and 20 seconds)
		+
		+	*Sent to sequencing:
		+	1)R0010+P0451 3A
		+	2)R0010+P0451 3D
		+
		+	*Gel Order:
		+	-Ladder
		+	-1A-E
		+	-2A-E
		+	-3A-B
		+
		+	-Ladder
		+	-3C-E
		+	-4A-E
	<center>[[Image:7-19-10.jpg]]</center>		<center>[[Image:7-19-10.jpg]]</center>
		+	*Transform:
		+	-C: Kit Plate 1 Well 3A
		+	-T: Kit Plate 1 Well 7A
		+	-A: Kit Plate 1 Well 1C
		+
		+
		+	==July 20th==
		+
		+
		+	*Digests:
		+	-J24679+B1006 (XP)
		+	-C0261 (ES)
		+	-LUX+RBS+B1006 (XP)
		+	-J23108 (ES)
		+	-J23116 (ES)
		+	-J23100 (ES)
		+	-pSB1C3 (EP+Phosphatase)
		+
		+	*Grow up:
		+	-J23116
		+	-J23108
		+
		+
		+	==July 21st==
		+
		+
		+	*Colony PCR:
		+	1)Lux+RBS+B1006+J23100+C3
		+	2)Lux+RBS+B1006+J23108+C3
		+	3)Lux+RBS+B1006+J23108+C3 redo
		+	4)Lux+RBS+B1006+J23116+C3
		+	5)J24679+B1006A+C0261+C3
		+	6)J24679+B1006C+C0261+C3
		+
		+	*Digest:
		+	-J24679 (ES)
		+	-P0451 (XP)
		+	-K3 (EP+Phosphatase)
		+	-B1006 (XP)
		+	-B1002 (XP)
		+	-R0010 (ES)
		+	-C3+rfp (EP+Phosphatase)
		+
		+	*Ligations:
		+	-J24679+K3
		+	-P0451+R0010
		+	-J24679+B1006
		+	-J24679+B1002
		+	-P0451+R0010
		+	-R0010+C3+rfp
		+
		+	Gel Order:
		+	-Ladder
		+	-4B-C
		+	-5A-C
		+	-6A-C
	<center>[[Image:7-21-10.jpg]]</center>		<center>[[Image:7-21-10.jpg]]</center>
		+	4C was grown up.
		+	==July 22nd==
		+
		+	*Colony PCR:
		+	1)J24679+B1006+K3
		+	2)P0451+R0010+RFP+C3
		+	3)J24679+B1002+K3
		+	(The extension time used was a minute and 40 seconds.)
		+	*Gel Order:
		+	-Ladder
		+	-Lux+RBS+B1006+J23100+C3 (A-C)
		+	-Lux+RBS+B1006+J23108+C3 (A-C)
		+	-Lux+RBS+B1006+J23108+C3 redo (A-C)
		+	-Lux+RBS+B1006+J23116+C3 (A)
	<center>[[Image:7-22-10.jpg]]</center>		<center>[[Image:7-22-10.jpg]]</center>
		+	*The colony 3C was grown up.
		+
		+
		+	*Gel Order:
		+	-Ladder
		+	-J24679+B1006+K3 (1A-E)
		+	-P0451+R0010+RFP+C3 (2A-E)
		+
		+	-Ladder
		+	-J24679+B1002+K3 (3A-3E)
	<center>[[Image:7-22-10 001.jpg]]</center>		<center>[[Image:7-22-10 001.jpg]]</center>
		+
		+	*Sent to sequencing:
		+	1)Lux+RBS+B1006+J23116+C3
		+	2)P0451+R0010+RFP+C3(A) (Forward and Reverse)
		+	3)pSB1T3 yellow (Forward)
		+	4) Lux+RBS+B1006 (Reverse)
		+
		+
		+
		+	==July 23rd==
		+
		+
		+	*Digest:
		+	-J24679 (ES)
		+	-B1002 (XP)
		+	-B1006 (XP)
		+	-Lux+RBS (ES)
		+	-C3 (EP+Phosphatase)
		+	-K3 (EP+Phosphatase)
		+
		+	*Ligations:
		+	-J24679+B1002
		+	-J24679+B1006
		+	-Lux+RBS+B1002
		+	-Lux+RBS+B1006
	<center>[[Image:7-22-10 002.jpg]]</center>		<center>[[Image:7-22-10 002.jpg]]</center>

---

Latest revision as of 15:52, 5 August 2010

Contents 1 July 19th 2 July 20th 3 July 21st 4 July 22nd 5 July 23rd

July 19th

• Colony PCR:

 -J24679+B1002+K3
 -J24679+B1006+K3
 -Lux+RBS+B1006+K3
 -Lux+RBS+B1002+K3
(The extension time used was a minute and 20 seconds)

• Sent to sequencing:

 1)R0010+P0451 3A
 2)R0010+P0451 3D

• Gel Order:

 -Ladder
 -1A-E
 -2A-E
 -3A-B

 -Ladder
 -3C-E
 -4A-E

• Transform:

 -C: Kit Plate 1 Well 3A
 -T: Kit Plate 1 Well 7A
 -A: Kit Plate 1 Well 1C

July 20th

• Digests:

 -J24679+B1006 (XP)
 -C0261 (ES)
 -LUX+RBS+B1006 (XP)
 -J23108 (ES)
 -J23116 (ES)
 -J23100 (ES)
 -pSB1C3 (EP+Phosphatase)

• Grow up:

 -J23116
 -J23108

July 21st

• Colony PCR:

 1)Lux+RBS+B1006+J23100+C3
 2)Lux+RBS+B1006+J23108+C3
 3)Lux+RBS+B1006+J23108+C3 redo
 4)Lux+RBS+B1006+J23116+C3
 5)J24679+B1006A+C0261+C3
 6)J24679+B1006C+C0261+C3

• Digest:

 -J24679 (ES)
 -P0451 (XP)
 -K3 (EP+Phosphatase)
 -B1006 (XP)
 -B1002 (XP)
 -R0010 (ES)
 -C3+rfp (EP+Phosphatase)

• Ligations:

 -J24679+K3
 -P0451+R0010
 -J24679+B1006
 -J24679+B1002
 -P0451+R0010
 -R0010+C3+rfp

Gel Order:

 -Ladder
 -4B-C
 -5A-C
 -6A-C

4C was grown up.

July 22nd

• Colony PCR:

 1)J24679+B1006+K3
 2)P0451+R0010+RFP+C3
 3)J24679+B1002+K3
(The extension time used was a minute and 40 seconds.)

• Gel Order:

 -Ladder
 -Lux+RBS+B1006+J23100+C3 (A-C)
 -Lux+RBS+B1006+J23108+C3 (A-C)
 -Lux+RBS+B1006+J23108+C3 redo (A-C)
 -Lux+RBS+B1006+J23116+C3 (A)

• The colony 3C was grown up.

• Gel Order:

 -Ladder
 -J24679+B1006+K3 (1A-E)
 -P0451+R0010+RFP+C3 (2A-E)

 -Ladder
 -J24679+B1002+K3 (3A-3E)

• Sent to sequencing:

 1)Lux+RBS+B1006+J23116+C3
 2)P0451+R0010+RFP+C3(A) (Forward and Reverse)
 3)pSB1T3 yellow (Forward)
 4) Lux+RBS+B1006 (Reverse)

July 23rd

• Digest:

 -J24679 (ES)
 -B1002 (XP)
 -B1006 (XP)
 -Lux+RBS (ES)
 -C3 (EP+Phosphatase)
 -K3 (EP+Phosphatase)

• Ligations:

 -J24679+B1002
 -J24679+B1006
 -Lux+RBS+B1002
 -Lux+RBS+B1006

Return to Notebook