Latest revision as of 20:28, 25 October 2010

Research

We initiated our research on subtilin quorum sensing system which would provide a coordinated cell response.

Klein C, Kaletta C and Entian KD. (1993). "Biosynthesis of the lantibiotic subtilin is regulated by a histidine kinase/response regulator system". Applied and environmental microbiology. 59(1). 296-303.
Heinzmann S, Entian KD and Stein T. (2006). "Engineering Bacillus subtilis ATCC 6633 for improved production of the lantibiotic subtilin". Applied microbiology and biotechnology. 69(5). 532-6.

@@ Line 1: / Line 1: @@
 {{Team:Newcastle/mainbanner}}
-= Screening for lacI insert =
+=Research=
-== Aim ==
+We initiated our research on subtilin quorum sensing system which would provide a coordinated cell response.
-The aim of this experiment is to screen for the lacI insert. This could be done using PCR but that would require specific primers and is not as reliable.
-== Protocol ==
+#Klein C, Kaletta C and Entian KD. (1993). "Biosynthesis of the lantibiotic subtilin is regulated by a histidine kinase/response regulator system". ''Applied and environmental microbiology''. 59(1). 296-303.
-*#Miniprep - the [[Team:Newcastle/Qiagen_Minipreps|miniprep]] protocol was followed.
+#Heinzmann S, Entian KD and Stein T. (2006). "Engineering Bacillus subtilis ATCC 6633 for improved production of the lantibiotic subtilin". ''Applied microbiology and biotechnology''. 69(5). 532-6.
-*#Restriction digests - the [[Team:Newcastle/Restriction_digests|restriction digests]] protocol was followed to allow the length of the DNA to be checked using single and double digest.
-*#*Single digest with Pst1
-*#*double digests with Pst1 and Xba1
-*#Gel electrophoresis - the [[Team:Newcastle/Gel_electrophoresis|gel electrophoresis]] protocol was followed.
-Inference
-*Set up liquid broth culture in LB
 {{Team:Newcastle/footer}}