Team:Stockholm/25 July 2010

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(New page: {{Stockholm/Top2}} ==Nina== ==Inoculation of IgG protease== The dish had 5 colonies, I inoculated these in 12 ml LB with 24 ul ampicillin resistance. When I know which colony is suitabl...)
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==Nina==
==Nina==
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==Inoculation of IgG protease==
+
===Inoculation of IgG protease===
-
The dish had 5 colonies, I inoculated these in 12 ml LB with 24 ul ampicillin resistance. When I know which colony is suitable I will miniprep it and send in to iGEM hq.
+
The dish had 5 colonies, I inoculated these in 12 ml LB with 24 ul chloramphenocol resistance (50mg/ml). When I know which colony is suitable I will miniprep it and send in to iGEM hq.
 +
 
 +
 
 +
 
 +
 
 +
== Mimmi ==
 +
 
 +
 
 +
=== LMWP ===
 +
 
 +
==== Re-doing the PCR ====
 +
 
 +
 
 +
* 25 colonies were checked
 +
** Nina and Johan were running the PCR, I made the gels...
 +
 
 +
 
 +
==== Gels ====
 +
 
 +
 
 +
[[Image:2010-07-25_pSB1C3.LMWP_clone_1-9.jpg|200px|thumb|left]]
 +
{|
 +
! well
 +
! sample
 +
|-
 +
| 1
 +
| ladder
 +
|-
 +
| 2
 +
| 1control
 +
|-
 +
| 3
 +
| 3 LMWP C
 +
|-
 +
| 4
 +
| 4 LMWP C
 +
|-
 +
| 5
 +
| 5 LMWP C (A)
 +
|-
 +
| 6
 +
| 6 LMWP C (B)
 +
|-
 +
| 7
 +
| 7 LMWP C (C)
 +
|-
 +
| 8
 +
|
 +
|-
 +
| 9
 +
| 9 LMWP N (C)
 +
|-
 +
|
 +
|}
 +
 
 +
 
 +
 
 +
[[Image:2010-07-25_pSB1C3.LMWP_clone_10-25.jpg|200px|thumb|left]]
 +
{|
 +
! well
 +
! sample
 +
! well
 +
! sample
 +
|-
 +
| 1
 +
| ladder
 +
| 12
 +
| ladder
 +
|-
 +
| 2
 +
| 10 LMWP N (B)
 +
| 13
 +
| empty
 +
|-
 +
| 3
 +
| 11 LMWP N (A)
 +
| 14
 +
| 18 LMWP N
 +
|-
 +
| 4
 +
| 12 LMWP N
 +
| 15
 +
| empty
 +
|-
 +
| 5
 +
| 13 LMWP N
 +
| 16
 +
| empty
 +
|-
 +
| 6
 +
| 14 LMWP N
 +
| 17
 +
| 19 LMWP N
 +
|-
 +
| 7
 +
| 15 LMWP N
 +
| 18
 +
| 20 LMWP N
 +
|-
 +
| 8
 +
| 16 LMWP N
 +
| 19
 +
| 21 LMWP N
 +
|-
 +
| 9
 +
| 17 LMWP N
 +
| 20
 +
| 22 LMWP N
 +
|-
 +
| 10
 +
| empty
 +
| 21
 +
| 23 LMWP N
 +
|-
 +
| 11
 +
| 25 LMWP N
 +
| 22
 +
| 24 LMWP N
 +
|}
 +
 
 +
{{Stockholm/Footer}}

Latest revision as of 10:52, 26 October 2010

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Contents

Nina

Inoculation of IgG protease

The dish had 5 colonies, I inoculated these in 12 ml LB with 24 ul chloramphenocol resistance (50mg/ml). When I know which colony is suitable I will miniprep it and send in to iGEM hq.



Mimmi

LMWP

Re-doing the PCR

  • 25 colonies were checked
    • Nina and Johan were running the PCR, I made the gels...


Gels

2010-07-25 pSB1C3.LMWP clone 1-9.jpg
well sample
1 ladder
2 1control
3 3 LMWP C
4 4 LMWP C
5 5 LMWP C (A)
6 6 LMWP C (B)
7 7 LMWP C (C)
8
9 9 LMWP N (C)


2010-07-25 pSB1C3.LMWP clone 10-25.jpg
well sample well sample
1 ladder 12 ladder
2 10 LMWP N (B) 13 empty
3 11 LMWP N (A) 14 18 LMWP N
4 12 LMWP N 15 empty
5 13 LMWP N 16 empty
6 14 LMWP N 17 19 LMWP N
7 15 LMWP N 18 20 LMWP N
8 16 LMWP N 19 21 LMWP N
9 17 LMWP N 20 22 LMWP N
10 empty 21 23 LMWP N
11 25 LMWP N 22 24 LMWP N





The Faculty of Science at Stockholm University Swedish Vitiligo association (Svenska Vitiligoförbundet) Geneious Fermentas/ Sigma-Aldrich/

Retrieved from "http://2010.igem.org/Team:Stockholm/25_July_2010"