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Team:Newcastle/Arginine test
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| - | ===Materials Required | + | {{Team:Newcastle/mainbanner}} |
| - | * Plate | + | |
| - | * Flame (streaking) | + | =Arginine Test= |
| - | * LB media consisting arginine | + | |
| + | ==Materials Required== | ||
| + | * Plate containing ''Bacillus subtilis'' 168 colonies. | ||
| + | * Flame (streaking) loop | ||
| + | * LB media consisting of arginine | ||
* Auto pipette | * Auto pipette | ||
* Bursen Burner | * Bursen Burner | ||
* Universal Tube | * Universal Tube | ||
| + | * pH indicator paper | ||
| - | == | + | ==Procedures== |
* Perform the experiment using aseptic technique. | * Perform the experiment using aseptic technique. | ||
* Transfer ''B. subtilis'' 168 colonies into universal tubes containing 5 ml of LB media and allowed to grow overnight at 37° C. | * Transfer ''B. subtilis'' 168 colonies into universal tubes containing 5 ml of LB media and allowed to grow overnight at 37° C. | ||
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#Test (2) - LB media with 10 mM of arginine plus ''B. subtilis'' 168 | #Test (2) - LB media with 10 mM of arginine plus ''B. subtilis'' 168 | ||
* Incubate the culture at 37° C with shaking. | * Incubate the culture at 37° C with shaking. | ||
| - | * Record the pH at every 30 min interval.Use 20 ul of the culture and measure the pH using the pH | + | * Record the pH at every 30 min interval.Use 20 ul of the culture and measure the pH using the pH indicator paper. |
| + | |||
| + | |||
| + | '''Go back to our [[Team:Newcastle/Protocol list|Protocol List]]''' | ||
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} | ||
Latest revision as of 20:58, 27 October 2010
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Arginine Test
Materials Required
- Plate containing Bacillus subtilis 168 colonies.
- Flame (streaking) loop
- LB media consisting of arginine
- Auto pipette
- Bursen Burner
- Universal Tube
- pH indicator paper
Procedures
- Perform the experiment using aseptic technique.
- Transfer B. subtilis 168 colonies into universal tubes containing 5 ml of LB media and allowed to grow overnight at 37° C.
- Transfer 1 ml of the overnight culture to another universal tube containing 4 ml of the following media:
- Control (1) - LB media
- Control (2) - LB media with 10 mM of arginine
- Control (3) - LB media plus B. subtilis 168
- Test (1) - LB media with 10 mM of arginine plus B. subtilis 168
- Test (2) - LB media with 10 mM of arginine plus B. subtilis 168
- Incubate the culture at 37° C with shaking.
- Record the pH at every 30 min interval.Use 20 ul of the culture and measure the pH using the pH indicator paper.
Go back to our Protocol List
   
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