Latest revision as of 20:50, 21 October 2010

Aims

The aim of today's Lab practice was to transform competent cells with GFP and RFP inserts in an RFP backbone.

Please refer to: Transformation of E. coli for materials required and protocol.

The aim of this Lab practice was to have lots of fun!

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 {{Team:Newcastle/mainbanner}}
-===Wednesday Morning===
+==Aims==
-====Aims====
 The aim of today's Lab practice was to transform competent cells with GFP and RFP inserts in an RFP backbone.
-====Materials====
+==Materials and Protocol==
-* ''E.coli'' broth culture
+Please refer to: [[Team:Newcastle/Transformation of E. coli|Transformation of ''E. coli'']] for materials required and protocol.
-* Pipettes
-* Plasmid ligation products from yesterday
-* Conical flasks
-====Protocol====
+= Wednesday afternoon - taking a break at the Hoppings!!! =
-Cells are made competent ready for transformation.
+==Aims==
-*Positive control(plasmid uncut)- to make sure cells are transfroming properly
-*Negative control (just cells)- nothing but competent cells
-microfuge tubes:
-*1:5
-*1:3
-*Vector
-*Negative add nothing
-*Positive add 1 &#0956;l uncut
-=== Wednesday afternoon - taking a break at the Hoppings!!! ===
-====Aims====
 The aim of this Lab practice was to have lots of fun!
-====Materials====
+==Materials==
 * Candy floss
 * Suntan lotion
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