Team:Newcastle/23 June 2010

From 2010.igem.org

(Difference between revisions)
(Mateials)
 
(18 intermediate revisions not shown)
Line 1: Line 1:
{{Team:Newcastle/mainbanner}}
{{Team:Newcastle/mainbanner}}
-
===Wednesday Morning===
+
==Aims==
-
 
+
-
====Aims====
+
The aim of today's Lab practice was to transform competent cells with GFP and RFP inserts in an RFP backbone.  
The aim of today's Lab practice was to transform competent cells with GFP and RFP inserts in an RFP backbone.  
-
====Equipment List====  
+
==Materials and Protocol==
-
* ''E.coli'' broth culture
+
Please refer to: [[Team:Newcastle/Transformation of E. coli|Transformation of ''E. coli'']] for materials required and protocol.
-
* Pipettes
+
-
* Plasmid ligation products from yesterday
+
-
* Conical flasks
+
-
====Transformation====  
+
= Wednesday afternoon - taking a break at the Hoppings!!! =
-
Cells are made competent ready for transformation.
+
==Aims==
-
*Positive control(plasmid uncut)- to make sure cells are transfroming properly
+
The aim of this Lab practice was to have lots of fun!
-
*Negative control (just cells)- nothing but competent cells
+
-
5 microfuge tubes:
+
==Materials==
-
*1:5
+
-
*1:3
+
-
*Vector
+
-
*Negative add nothing
+
-
*Positive add 1μl uncut
+
-
 
+
-
=== Wednesday afternoon taking a break ===
+
-
 
+
-
====Aims====
+
-
The aim of this Lab practice was to have fun.
+
-
 
+
-
====Materials====
+
* Candy floss  
* Candy floss  
* Suntan lotion
* Suntan lotion
Line 51: Line 33:
|-
|-
|}
|}
-
 

Latest revision as of 20:50, 21 October 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Contents

Aims

The aim of today's Lab practice was to transform competent cells with GFP and RFP inserts in an RFP backbone.

Materials and Protocol

Please refer to: Transformation of E. coli for materials required and protocol.

Wednesday afternoon - taking a break at the Hoppings!!!

Aims

The aim of this Lab practice was to have lots of fun!

Materials

  • Candy floss
  • Suntan lotion
  • Teddy bears
  • Burgers and bacon
  • Chips and gravy
  • Icecream
  • Meerkat toys
  • Madmouse ride!
  • Beer, wine, lemonade
  • Football


Newcastle iGEM funfair.jpeg Newcastle iGEM funfair2.jpeg Newcastle iGEM funfair3.jpeg



Newcastle Hoppings 001.JPG
Call to arms!
Philamentous!!!
Newcastle Hoppings 004.JPG
Newcastle Hoppings 005.JPG
Newcastle Hoppings 006.JPG
Newcastle Hoppings 008.JPG
Newcastle Hoppings 009.JPG
Newcastle Hoppings 010.JPG
Newcastle Hoppings 011.JPG
Newcastle Hoppings 012.JPG
Newcastle Hoppings 013.JPG
Newcastle Hoppings 014.JPG
Newcastle Hoppings 015.JPG
Newcastle Hoppings 016.JPG
Newcastle Hoppings 017.JPG
Newcastle Hoppings 018.JPG
Newcastle Hoppings 019.JPG
Newcastle Hoppings 022.JPG
Newcastle Hoppings 024.JPG
Newcastle University logo.png    Newcastle cbcb logo.pngNewcastle Biomedicine logo.gif    Team Newcastle CEG logo.gif
Newcastle iww logo.jpg  UNIPV Pavia Logo.gif  Newcastle BBSRC.gif    Newcastle Genevision logo.png Newcastle WelcomeTrust.jpg
FaceBook Icon