Making Media
From 2010.igem.org
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* 10ml Deionized Water | * 10ml Deionized Water | ||
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+ | [https://2010.igem.org/Team:Penn_State/Notebook Return to Notebook] |
Latest revision as of 19:58, 29 July 2010
LB Media (RT)
* 10g Tryptone * 10g NaCl * 5g Yeast Extract * 1L Deionized Water
SOB Media (RT)
* 20g Tryptone * 5g Yeast Extract * 0.5g NaCl * 0.2g KCl * 1L Deionized Water
SOC Media (4 degrees Celsius)
* Add the following per ml SOB: - 4ul MgCl2 Solution (100) - 8ul Glucose Solution (200)
Glucose Stock Solution (4 degrees Celsius)
* 5g glucose * 10ml Deionized Water