Latest revision as of 22:08, 27 October 2010

BCCS-Bristol
iGEM 2010

Wet lab

• Achievements • Part Design • Lab Work • Beads • Safety • Improvements •

Our Labspace

Our main achievement in the wet lab has been the creation of a new composite BioBrick, engineering E. coli to produce GFP in response to Nitrates. Not only is this part complete, but it's also well characterised both on its own and working in tandem with a second BioBrick.

We also developed a way of encapsulating our bacteria, allowing an easy and safe method of spreading them in the environment, and also dramatically improving signal detection and E. coli survival in soil.

Finally we worked to further characterise the PyeaR promoter submitted by Edinburgh in 2009, improving the quality of information available in the parts registry.

In this section you can find out more details on all our achievements. You can also find information about about the design of our parts, including all the decisions we made and why, as well as all the information relating to their construction and characterisation. It also contains material on our new encapsulation methods, and finally lists all our safety considerations both in the lab and on a wider environmental scale.

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-== Wet lab ==
+{{:Team:BCCS-Bristol/Header}}
+=Wet lab=
-'''09/07/2010 - Day One'''
+<center> • [[:Team:BCCS-Bristol/Wetlab/Achievements|Achievements]] • [[:Team:BCCS-Bristol/Wetlab/Part_Design|Part Design]] • [[:Team:BCCS-Bristol/Wetlab/Experiments|Lab Work]] •
+[[:Team:BCCS-Bristol/Wetlab/Beads|Beads]] • [[:Team:BCCS-Bristol/Wetlab/Safety|Safety]] • [[:Team:BCCS-Bristol/Wetlab/Improvements|Improvements]] • </center>
-Made two different competent strains of ''E.coli'', and attempted transformations on both using BBa_I13522 (pTet GFP) from the kit - prepared 6 plates: 1 positive control for each strain, 1 negative control for each strain and 1 test transformation plate for each strain. The positive control was carried out using a plasmid of known quantity. Ampicillin was used for selection.
-'''12/07/2010 - Day Two'''
+[[Image:Picture_018.jpg|right|thumbnail|250px|Our Labspace]]
-No growth on test plates.
+Our main achievement in the wet lab has been the creation of a new composite BioBrick, engineering ''E. coli'' to produce GFP in response to Nitrates. Not only is this part complete, but it's also well characterised both on its own and working in tandem with a second BioBrick.
-==Safety issues==
+We also developed a way of encapsulating our bacteria, allowing an easy and safe method of spreading them in the environment, and also dramatically improving signal detection and ''E. coli'' survival in soil.
-As the idea of communication between a certain population (in this case E.coli) could raise issues in health and safety of the general public the following precautions were taken during the implementation of the project in the laboratory:
-*Novel proteins handled (FhuA,OsmE,Fiu) were derived from DNA by gene cloning with PCR from E.coli MG1655 a laboratory strain with no toxic implications.
+Finally we worked to further characterise the PyeaR promoter submitted by Edinburgh in 2009, improving the quality of information available in the parts registry.
-*Novel proteins handled where screened from the literature to ensure that they will have no toxicity effects.
-*Experiments were implemented in a Level 1 Laboratory with access only by trained individuals.
-*Students involved in experimental work in the laboratory were trained to an appropriate level  to apply relevant techniques and use relevant equipment  and where suitable were supervised whilst carrying out laboratory work.
+In this section you can find out more details on all our achievements. You can also find information about about the design of our parts, including all the decisions we made and why, as well as all the information relating to their construction and characterisation. It also contains material on our new encapsulation methods, and finally lists all our safety considerations both in the lab and on a wider environmental scale.
-*Laboratory workers were always clothed in appropriate manner (lab coat, gloves, safety spectacles).
-*Laboratory workers sterilised their hands before and after laboratory work and before entering and exiting the lab at all times.
-*No bacterial cultures exited the laboratory unless they were suitably packaged and accompanied by one of the team members whilst in transport and this only occurred where it was necessary to transport cultures from one laboratory to another.
-*No purified DNA or biological material was left unattended at any time, and all DNA and biological material was suitably stored according to Level 1 Laboratory rules.
-*Biosafety guidelines where followed under the BCCS-Bristol iGEM'09 supervising team and such guidelines fall within the description of a project that holds approval by the iGEM supervisor Dr.Nigel Savery.