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Team:Groningen/4 October 2010
From 2010.igem.org
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A expression experiment was performed to get samples for the mass spectometry. | A expression experiment was performed to get samples for the mass spectometry. | ||
<br> | <br> | ||
| - | Strain E and deltaTasA were grown in TY over night. The next morning OD was measured and both strains were diluted in fresh TY with antibiotics in 100 mL. | + | Strain E and deltaTasA were grown in TY over night. The next morning OD was measured and both strains were diluted in fresh TY with antibiotics in 100 mL and divided into dTasA, E and E non induced. |
| + | |||
| + | OD was measured every hour, at approximately OD = 0,5 (after 2,5 hours) 1% subtilin was addded to the E-culture and from then onward every 2 hours OD was measured. | ||
| + | |||
| + | After five hours the OD was measured again and samples were taken for preperation: | ||
| + | |||
| + | The following groups were prepared out of 2 mL of culture: | ||
| + | |||
| + | E/dTasA/EN pellet | ||
| + | E/dTasA/EN pellet, SDS | ||
| + | E/dTasA/EN pellet, Lysozyme | ||
| + | E/dTasA/EN pellet, Lysozyme SDS | ||
| + | |||
| + | E/dTasA/EN supernatant | ||
| + | E/dTasA/EN supernatant, SDS | ||
| + | E/dTasA/EN supernatant, Lysozyme | ||
| + | E/dTasA/EN supernatant, Lysozyme SDS | ||
| + | |||
| + | Samples were stored in the freezer to await further analysis using mass spec. | ||
| + | |||
| + | '''Week 41''' | ||
| + | |||
| + | <br> | ||
| + | |||
| + | |||
| + | '''Week 43''' | ||
| + | |||
| + | <br> | ||
| + | |||
| + | '''Expression experiment David & Peter''' | ||
| + | |||
| + | <br> | ||
| + | |||
| + | Massspec data for the induced cultures of E, EN, C and CN was aquired, with the help of Sander we performed Malditov mass spectometry on both these samples and the samples in stock from the large E induction experiment. The samples of the E-induction experiment gave no data. | ||
| + | |||
| + | <br> | ||
| + | |||
| + | [[Image:MassSpecGR.jpg]] | ||
| + | |||
| + | <br> | ||
| + | |||
<br> | <br> | ||
Latest revision as of 02:22, 28 October 2010
Hydrophobofilm
pushing coatings into a greener future
Week 40
Expression-Peter and David
A expression experiment was performed to get samples for the mass spectometry.
Strain E and deltaTasA were grown in TY over night. The next morning OD was measured and both strains were diluted in fresh TY with antibiotics in 100 mL and divided into dTasA, E and E non induced.
OD was measured every hour, at approximately OD = 0,5 (after 2,5 hours) 1% subtilin was addded to the E-culture and from then onward every 2 hours OD was measured.
After five hours the OD was measured again and samples were taken for preperation:
The following groups were prepared out of 2 mL of culture:
E/dTasA/EN pellet E/dTasA/EN pellet, SDS E/dTasA/EN pellet, Lysozyme E/dTasA/EN pellet, Lysozyme SDS
E/dTasA/EN supernatant E/dTasA/EN supernatant, SDS E/dTasA/EN supernatant, Lysozyme E/dTasA/EN supernatant, Lysozyme SDS
Samples were stored in the freezer to await further analysis using mass spec.
Week 41
Week 43
Expression experiment David & Peter
Massspec data for the induced cultures of E, EN, C and CN was aquired, with the help of Sander we performed Malditov mass spectometry on both these samples and the samples in stock from the large E induction experiment. The samples of the E-induction experiment gave no data.
Laura
Expanding gene expression model and programmed this in Matlab, searched for constants.
Arend Information standard ready for submission
