Team:Newcastle/Meetings/12 July 2010

From 2010.igem.org

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*Informal meeting at the Walton Library [3.30-5.00pm]
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{{Team:Newcastle/mainbanner}}
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*Chair: Da, Minutes: Younus
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==Cloning Strategies==
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==Informal meeting - 12th July 2010==
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====5 parts to a Cloning Strategy:====
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*Chair: Da, Minutes: Younus
-
The cloning strategies for each biobrick should contain the following sections in this order:
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*Walton Library [3.30 - 5.00pm]
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#Purpose and Justification
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-
#Modelling
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-
#Construction
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-
#Cloning and Integration
+
-
#Testing and Characterising
+
 +
===Cloning Strategies===
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====Required Cloning Strategies====
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=====5 parts to a Cloning Strategy:=====
-
There are 8 cloning strategies which are required:
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*The cloning strategies for each biobrick should contain the following sections in this order:
 +
*#Purpose and Justification
 +
*#Modelling
 +
*#Construction
 +
*#Cloning and Integration
 +
*#Testing and Characterising
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*'''SR1''' - finished!
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=====Required Cloning Strategies=====
-
*'''YneA''' - biobrick nearly coming back - our most important biobrick!
+
*There are 8 cloning strategies which are required:
-
*'''LacI''' - not sure how to test this biobrick
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**'''SR1''' - finished!
-
*'''2 for Swarming''' (sfp and swrA) - cloning strategies nearly finished
+
**'''YneA''' - biobrick nearly coming back - our most important biobrick!
-
*'''2 for Subtilin''' (immunity and production) - cloning strategies nearly finished
+
**'''LacI''' - not sure how to test this biobrick
-
*'''RocF''' (need to use Gibson method) - not started
+
**'''2 for Swarming''' (sfp and swrA) - cloning strategies nearly finished
 +
**'''2 for Subtilin''' (immunity and production) - cloning strategies nearly finished
 +
**'''RocF''' (need to use Gibson method) - not started
So only the cloning strategy for SR1 is finished. 7 more to go!
So only the cloning strategy for SR1 is finished. 7 more to go!
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=====Allocation of team members to cloning strategies:=====
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====Allocation of team members to cloning strategies:====
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*'''YneA''' - Phil, Rach, Deena, Younus
*'''YneA''' - Phil, Rach, Deena, Younus
 +
*'''LacI''' - Phil, Younus, Rachel
*'''Swarming (2)''' - Harsh, Alan, Steve, Da
*'''Swarming (2)''' - Harsh, Alan, Steve, Da
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*'''LacI''' - Phil, Younus, Rachel
 
*'''Subtilin (2)''' - Harsh, Alan, Steve, Deena
*'''Subtilin (2)''' - Harsh, Alan, Steve, Deena
*'''RocF''' - Harsh, Alan, Steve, Da
*'''RocF''' - Harsh, Alan, Steve, Da
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====Wiki - Labbook====
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==Wiki==
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====Labbook====
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Everyone needs to carry out the following protocol for each day in the lab
Everyone needs to carry out the following protocol for each day in the lab
#Aim
#Aim
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*Wiki checkers: Deena and Rachel (after altering/updating a page on the wiki, team members should send the links to the pages to the wiki checkers)
*Wiki checkers: Deena and Rachel (after altering/updating a page on the wiki, team members should send the links to the pages to the wiki checkers)
 +
===UK iGEM Gathering at Newcastle - 20th/21st July===
 +
====Presentation:====
 +
=====Introduction=====
 +
*Rach, Deena, Jannetta [2 mins]
 +
**Cover Page
 +
**Picture of us standing up
 +
**Introduction of our iGEM project - using BS to repair concrete
 +
=====General Outlook=====
 +
*Harsh, Da, Phil [5 mins]
 +
**Starting with bioreactor
 +
**Transformation into spores
 +
**Transportation
 +
**Spraying on building
 +
**Germination
 +
**sensing crack + non-environmental kill switch
 +
**swarming
 +
**end of crack signalling = QS
 +
**Filamentous cells
 +
**CaCO3
 +
=====BioBrick Discussion=====
 +
*Alan, Younus, Steve [8 mins altogether; 2 mins each]
 +
**Swarming
 +
**Subtillin
 +
**Filamentous cells - YneA
 +
**CaCO3 - Urease
 +
**LacI
 +
=====Whats next...=====
 +
*[5 mins remaining]
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**'Save the babies!!!'
-
 
+
===Minideadlines===
-
==UK iGEM Gathering at Newcastle - 20th/21st July==
+
#Presentation slides to be e-mailed by tomorrow (Wed) 5 pm
-
 
+
#Cloning Strategies - to be completed by Friday, 16th July 2010.
-
 
+
-
===Presentation:===
+
-
====Introduction====
+
-
[2 mins] Rach, Deena, Jannetta
+
-
*Cover Page
+
-
*Picture of us standing up
+
-
*Introduction of our iGEM project - using BS to repair concrete
+
-
 
+
-
====General Outlook====
+
-
[5 mins] Harsh, Da, Phil
+
-
*Starting with bioreactor
+
-
*Transformation into spores
+
-
*Transportation
+
-
*Spraying on building
+
-
*Germination
+
-
*sensing crack + non-environmental kill switch
+
-
*swarming
+
-
*end of crack signalling = QS
+
-
*Filamentous cells
+
-
*CaCO3
+
-
*Glue
+
-
*Kill Switch
+
-
 
+
-
====BioBrick Discussion====
+
-
[8 mins altogether; 2 mins each] Alan, Younus, Steve
+
-
*Swarming
+
-
*Subtillin
+
-
*Filamentous cells - YneA
+
-
*CaCO3 - Urease
+
-
 
+
-
(5 mins remaining)
+
-
 
+
-
====Whats next...====
+
-
'Save the babies!!!'
+
-
 
+
-
 
+
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==Minideadlines==
+
-
#Cloning Strategies  
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#*YneA
#*YneA
#*One sequence for sfp primers and coding sequence ready (Goksel)
#*One sequence for sfp primers and coding sequence ready (Goksel)
#*Subtilin just immunity
#*Subtilin just immunity
-
 
+
===Action Points===
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==Action Points==
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#Finish wiki updates by Sunday 18th (everyone)
#Finish wiki updates by Sunday 18th (everyone)
#Team page on Wiki:
#Team page on Wiki:
-
#*Add Colin Harwood, Colin Daviy, Wendy Smith and Goksel Misrili to list of advisors
+
#*Add Colin Harwood, Colin Davies, Wendy Smith and Goksel Misrili to list of advisors
#*Deena, Phil and Younus to write about yourself + pictures
#*Deena, Phil and Younus to write about yourself + pictures

Latest revision as of 18:34, 25 October 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Contents

Informal meeting - 12th July 2010

  • Chair: Da, Minutes: Younus
  • Walton Library [3.30 - 5.00pm]

Cloning Strategies

5 parts to a Cloning Strategy:
  • The cloning strategies for each biobrick should contain the following sections in this order:
    1. Purpose and Justification
    2. Modelling
    3. Construction
    4. Cloning and Integration
    5. Testing and Characterising
Required Cloning Strategies
  • There are 8 cloning strategies which are required:
    • SR1 - finished!
    • YneA - biobrick nearly coming back - our most important biobrick!
    • LacI - not sure how to test this biobrick
    • 2 for Swarming (sfp and swrA) - cloning strategies nearly finished
    • 2 for Subtilin (immunity and production) - cloning strategies nearly finished
    • RocF (need to use Gibson method) - not started

So only the cloning strategy for SR1 is finished. 7 more to go!

Allocation of team members to cloning strategies:
  • YneA - Phil, Rach, Deena, Younus
  • LacI - Phil, Younus, Rachel
  • Swarming (2) - Harsh, Alan, Steve, Da
  • Subtilin (2) - Harsh, Alan, Steve, Deena
  • RocF - Harsh, Alan, Steve, Da

Wiki - Labbook

Everyone needs to carry out the following protocol for each day in the lab

  1. Aim
  2. Materials and Methods
  3. Procedure/Protocol
  4. Results
  5. Conclusion

Relevant photographs should be added and labelled

General:

  • Wiki checkers: Deena and Rachel (after altering/updating a page on the wiki, team members should send the links to the pages to the wiki checkers)

UK iGEM Gathering at Newcastle - 20th/21st July

Presentation:

Introduction
  • Rach, Deena, Jannetta [2 mins]
    • Cover Page
    • Picture of us standing up
    • Introduction of our iGEM project - using BS to repair concrete
General Outlook
  • Harsh, Da, Phil [5 mins]
    • Starting with bioreactor
    • Transformation into spores
    • Transportation
    • Spraying on building
    • Germination
    • sensing crack + non-environmental kill switch
    • swarming
    • end of crack signalling = QS
    • Filamentous cells
    • CaCO3
BioBrick Discussion
  • Alan, Younus, Steve [8 mins altogether; 2 mins each]
    • Swarming
    • Subtillin
    • Filamentous cells - YneA
    • CaCO3 - Urease
    • LacI
Whats next...
  • [5 mins remaining]
    • 'Save the babies!!!'

Minideadlines

  1. Presentation slides to be e-mailed by tomorrow (Wed) 5 pm
  2. Cloning Strategies - to be completed by Friday, 16th July 2010.
    • YneA
    • One sequence for sfp primers and coding sequence ready (Goksel)
    • Subtilin just immunity

Action Points

  1. Finish wiki updates by Sunday 18th (everyone)
  2. Team page on Wiki:
    • Add Colin Harwood, Colin Davies, Wendy Smith and Goksel Misrili to list of advisors
    • Deena, Phil and Younus to write about yourself + pictures
Retrieved from "http://2010.igem.org/Team:Newcastle/Meetings/12_July_2010"