Team:Newcastle/Meetings/12 July 2010

From 2010.igem.org

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Chair: Da, Minutes: Younus, Comp
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{{Team:Newcastle/mainbanner}}
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#. Cloning Strategies
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==Informal meeting - 12th July 2010==
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*SR1 - Finished
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*RocF not started - need to use Gibson method;
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*YneA
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*2 for Swarming (sfp and swrA)
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*LacI
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*2 for Subtilin (Immunity and Production)
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Only finished one, SR1. 7 more to go!
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*Chair: Da, Minutes: Younus
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*Walton Library [3.30 - 5.00pm]
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Next: swarming and subtilin nearly finished, YneA nearly coming back and LacI brick not sure how to test.
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===Cloning Strategies===
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Why is testing LacI hard?
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*YneA - our most important BB - Phil, Rach, Deena, Younus
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=====5 parts to a Cloning Strategy:=====
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*Swarming (2) - Harsh, Alan, Steve, Da
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*The cloning strategies for each biobrick should contain the following sections in this order:
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*LacI - Phil, Younus, Rachel
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*#Purpose and Justification
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*Subtilin (2) - Harsh, Alan, Steve, Deena
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*#Modelling
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*RocF - Harsh, Alan, Steve, Da
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*#Construction
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*#Cloning and Integration
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*#Testing and Characterising
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5 parts to a Cloning Strategy
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=====Required Cloning Strategies=====
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#Purpose and justification
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*There are 8 cloning strategies which are required:
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#Modelling
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**'''SR1''' - finished!
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#Construction
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**'''YneA''' - biobrick nearly coming back - our most important biobrick!
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#Cloning and Integration
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**'''LacI''' - not sure how to test this biobrick
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#Testing and Characterising
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**'''2 for Swarming''' (sfp and swrA) - cloning strategies nearly finished
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**'''2 for Subtilin''' (immunity and production) - cloning strategies nearly finished
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**'''RocF''' (need to use Gibson method) - not started
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So only the cloning strategy for SR1 is finished. 7 more to go!
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#. Wiki
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=====Allocation of team members to cloning strategies:=====
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Labbook
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*'''YneA''' - Phil, Rach, Deena, Younus
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#. Aim
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*'''LacI''' - Phil, Younus, Rachel
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#. Materials and Methods
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*'''Swarming (2)''' - Harsh, Alan, Steve, Da
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#. Procedure/Protocol
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*'''Subtilin (2)''' - Harsh, Alan, Steve, Deena
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#. Results
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*'''RocF''' - Harsh, Alan, Steve, Da
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#. Conclusion
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Everyone needs to carry out this protocol above
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Photographs add and label them
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In charge of wiki - Deena and Rachel (everyone everyday they have to send the links to both of them to check)
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'''20th July - UK iGEM Gathering at Newcastle!'''
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====Wiki - Labbook====
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Everyone needs to carry out the following protocol for each day in the lab
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#Aim
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#Materials and Methods
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#Procedure/Protocol
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#Results
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#Conclusion
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Relevant photographs should be added and labelled
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'''PRESENTATION'''
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====General:====
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'''Introduction''' - Rach, Deena, Jannetta
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*Wiki checkers: Deena and Rachel (after altering/updating a page on the wiki, team members should send the links to the pages to the wiki checkers)
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*Cover Page
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*Picture of standing us up
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*Introduction of our iGEM project - using BS to repair concrete (2 mins)
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===UK iGEM Gathering at Newcastle - 20th/21st July===
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====Presentation:====
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=====Introduction=====
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*Rach, Deena, Jannetta [2 mins]
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**Cover Page
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**Picture of us standing up
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**Introduction of our iGEM project - using BS to repair concrete
 +
=====General Outlook=====
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*Harsh, Da, Phil [5 mins]
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**Starting with bioreactor
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**Transformation into spores
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**Transportation
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**Spraying on building
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**Germination
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**sensing crack + non-environmental kill switch
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**swarming
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**end of crack signalling = QS
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**Filamentous cells
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**CaCO3
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=====BioBrick Discussion=====
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*Alan, Younus, Steve [8 mins altogether; 2 mins each]
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**Swarming
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**Subtillin
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**Filamentous cells - YneA
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**CaCO3 - Urease
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**LacI
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=====Whats next...=====
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*[5 mins remaining]
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**'Save the babies!!!'
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'''General Outlook''' (5 mins):
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===Minideadlines===
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*Starting with bioreactor
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#Presentation slides to be e-mailed by tomorrow (Wed) 5 pm
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*Transformation into spores
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#Cloning Strategies - to be completed by Friday, 16th July 2010.
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*Transportation
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#*YneA
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*Spraying on building
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#*One sequence for sfp primers and coding sequence ready (Goksel)
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*Germination
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#*Subtilin just immunity
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*sensing crack + non-environmental kill switch
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*swarming
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*end of crack signalling = QS
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*Filamentous cells
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*CaCO3
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*Glue
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*Kill Switch
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'''BioBrick Discussion''' (8 mins altogether; 2 mins each):
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===Action Points===
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*Swarming
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#Finish wiki updates by Sunday 18th (everyone)
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*Subtillin
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#Team page on Wiki:
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*Filamentous cells - YneA
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#*Add Colin Harwood, Colin Davies, Wendy Smith and Goksel Misrili to list of advisors
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*CaCo3 - Urease
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#*Deena, Phil and Younus to write about yourself + pictures
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(5 mins remaining)
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'''Whats next'''...
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'Save the babies!!!'
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Latest revision as of 18:34, 25 October 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Contents

Informal meeting - 12th July 2010

  • Chair: Da, Minutes: Younus
  • Walton Library [3.30 - 5.00pm]

Cloning Strategies

5 parts to a Cloning Strategy:
  • The cloning strategies for each biobrick should contain the following sections in this order:
    1. Purpose and Justification
    2. Modelling
    3. Construction
    4. Cloning and Integration
    5. Testing and Characterising
Required Cloning Strategies
  • There are 8 cloning strategies which are required:
    • SR1 - finished!
    • YneA - biobrick nearly coming back - our most important biobrick!
    • LacI - not sure how to test this biobrick
    • 2 for Swarming (sfp and swrA) - cloning strategies nearly finished
    • 2 for Subtilin (immunity and production) - cloning strategies nearly finished
    • RocF (need to use Gibson method) - not started

So only the cloning strategy for SR1 is finished. 7 more to go!

Allocation of team members to cloning strategies:
  • YneA - Phil, Rach, Deena, Younus
  • LacI - Phil, Younus, Rachel
  • Swarming (2) - Harsh, Alan, Steve, Da
  • Subtilin (2) - Harsh, Alan, Steve, Deena
  • RocF - Harsh, Alan, Steve, Da

Wiki - Labbook

Everyone needs to carry out the following protocol for each day in the lab

  1. Aim
  2. Materials and Methods
  3. Procedure/Protocol
  4. Results
  5. Conclusion

Relevant photographs should be added and labelled

General:

  • Wiki checkers: Deena and Rachel (after altering/updating a page on the wiki, team members should send the links to the pages to the wiki checkers)

UK iGEM Gathering at Newcastle - 20th/21st July

Presentation:

Introduction
  • Rach, Deena, Jannetta [2 mins]
    • Cover Page
    • Picture of us standing up
    • Introduction of our iGEM project - using BS to repair concrete
General Outlook
  • Harsh, Da, Phil [5 mins]
    • Starting with bioreactor
    • Transformation into spores
    • Transportation
    • Spraying on building
    • Germination
    • sensing crack + non-environmental kill switch
    • swarming
    • end of crack signalling = QS
    • Filamentous cells
    • CaCO3
BioBrick Discussion
  • Alan, Younus, Steve [8 mins altogether; 2 mins each]
    • Swarming
    • Subtillin
    • Filamentous cells - YneA
    • CaCO3 - Urease
    • LacI
Whats next...
  • [5 mins remaining]
    • 'Save the babies!!!'

Minideadlines

  1. Presentation slides to be e-mailed by tomorrow (Wed) 5 pm
  2. Cloning Strategies - to be completed by Friday, 16th July 2010.
    • YneA
    • One sequence for sfp primers and coding sequence ready (Goksel)
    • Subtilin just immunity

Action Points

  1. Finish wiki updates by Sunday 18th (everyone)
  2. Team page on Wiki:
    • Add Colin Harwood, Colin Davies, Wendy Smith and Goksel Misrili to list of advisors
    • Deena, Phil and Younus to write about yourself + pictures
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