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 {{Team:Newcastle/mainbanner}}
-==Polymerase Chain Reaction protocol==
-Polymerase chain reaction (PCR) is a technique used to amplify a single or few a copies of a piece of DNA generating thousands to millions of copies of a particular DNA sequence.
+=Polymerase Chain Reaction=
-# To avoid contamination, wear gloves
-# To achieve optimum results, always do everything on ice
-A basic PCR set up requires several components and reagents.These components include:
+==Aim==
-#DNA template that contains the DNA region (target) to be amplified.
-#Two primers that are complementary to the 3' (three prime) ends of each of the sense and anti-sense strand of the DNA target.
-#Taq polymerase or another DNA polymerase with a temperature optimum at around 70 °C.
-#Deoxynucleoside triphosphates, the building blocks from which the DNA polymerases synthesizes a new DNA strand.
-#Buffer solution, providing a suitable chemical environment for optimum activity and stability of the DNA polymerase.
-[[Image:fri-gel.png|400px]] [[Image:Newcastle gel 20100625.jpg|400px]]
+To amplify a desired DNA fragment by using a set of appropriate primers flanking the fragment.
+==Materials and Protocol==
+Please refer to: [[Team:Newcastle/PCR| PCR]] for materials required and protocol.
+==Result==
+[[Image:8062010.jpg|400px|center]]
+'''Image 1''': Image of the gel showing PCR products.
+* '''Lane 1''': 1kb DNA ladder
+* '''Lane 2''': Sample 1
+* '''Lane 3''': Sample 2
+* '''Lane 4''': Sample 3
+* '''Lane 5''': Sample 4
+* '''Lane 6''': Sample 5
+* '''Lane 7''': Sample 6
+* '''Lane 8''': Sample 7
+* '''Lane 9''': Sample 8
+* '''Lane 10''': Sample 9
+* '''Lane 11''': Sample 10
+* '''Lane 12''': Sample 11
+* '''Lane 13''': Sample 12
+* '''Lane 14''': Sample 13
+* '''Lane 15''': Sample 14
+{{Team:Newcastle/footer}}

Team:Newcastle/25 June 2010

From 2010.igem.org

Latest revision as of 13:54, 27 October 2010

Contents

Polymerase Chain Reaction

Aim

Materials and Protocol

Result