Team:UC Davis/notebook/assembly.html
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<a name="assembly"></a><p class="header"><b>Assembly Workplans</b></a><p> | <a name="assembly"></a><p class="header"><b>Assembly Workplans</b></a><p> | ||
+ | <a name="pieces"></a><p class="header"><b>Creating Each Individual Composite Part</b></a><p> | ||
+ | |||
+ | <b>**Click on the individual pieces below to view how we ligated them together.**</b> <p><br /> | ||
+ | <b>Miscellaneous Parts</b></a><br /> | ||
<table border=0 margin="0" width="420px" padding:0 margin:0> | <table border=0 margin="0" width="420px" padding:0 margin:0> | ||
<tr> | <tr> | ||
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</table><p><br /> | </table><p><br /> | ||
+ | <b>Genetic Circuit Pieces</b><br /> | ||
+ | <table border=0 margin="0" width="405px" padding:0 margin:0> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <table> | ||
+ | <tr> | ||
+ | <td width="200px"><a href="https://2010.igem.org/Team:UC_Davis/notebook/initiatorx.html" class="help"><img src="https://static.igem.org/mediawiki/2010/0/0a/Initiatorbutton.jpg"></a></td> | ||
+ | <td width="200px"><a href="https://2010.igem.org/Team:UC_Davis/notebook/lassensor.html" class="help"><img src="https://static.igem.org/mediawiki/2010/7/72/Lassensorbutton.jpg"></a></td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="https://2010.igem.org/Team:UC_Davis/notebook/onpathway.html" class="help"><img src="https://static.igem.org/mediawiki/2010/d/d8/Onswitchbutton.jpg"></a></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="https://2010.igem.org/Team:UC_Davis/notebook/luxpathway.html" class="help"><img src="https://static.igem.org/mediawiki/2010/2/23/Offswitchbutton.jpg"></a></td> | ||
+ | </tr> | ||
+ | </table><p><br /> | ||
+ | <a name="plasmid"></a><p class="header"><b>Next Mission: Placing them into only two plasmids</b></a><p> | ||
+ | |||
+ | <img src="https://static.igem.org/mediawiki/2010/3/36/Plasmid1.jpg"><br /> | ||
+ | <img src="https://static.igem.org/mediawiki/2010/1/12/Plasmid2.jpg"><p><br /> | ||
+ | |||
+ | |||
+ | <a name="topagar"></a><p class="header"><b>Final Mission: Create an Even Lawn of E. Coli</b><br /> | ||
<ul> | <ul> | ||
- | <li><a href="https://2010.igem.org/Team:UC_Davis/ | + | <li>We are using this <a href="https://2010.igem.org/Team:UC_Davis/protocols/topagar.html" class="help">top agar plating technique </a></li> |
- | <li> | + | <li>Next is to test the what the optimal OD, arabinose concentration, and amount of light it takes to a) create an even lawn of E. Coli and b) see contrast between the "on" and "off" cells </li> |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
</ul><p><br /> | </ul><p><br /> | ||
- | |||
- | < | + | <a name="protocols"></a><p class="header"><b>Basic Assembly Protocols</b></a><p> |
+ | <b>For more insight about the process behind the ligations, here is a sample ligation procedure. Because the initiator is one of the smaller constructs, we have decided to use it as an example to illustrate the protocols used in lab.</b><p> | ||
- | + | <img src="https://static.igem.org/mediawiki/2010/2/29/Initiatorconstruct.jpg"><p> | |
<a name="startup"><h1>Starting Up</h1></a> | <a name="startup"><h1>Starting Up</h1></a> | ||
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<td class="kirby"> | <td class="kirby"> | ||
<ul> | <ul> | ||
- | <li>Assembly Workplan</li> | + | <li><a href="#assembly" class="help">Assembly Workplan</li> |
+ | <li><a href="#pieces" class="help">Creating Each Composite Part</li> | ||
+ | <li><a href="#plasmid" class="help">Placing Into Two Plasmids</li> | ||
+ | <li><a href="#topagar" class="help">Creating Lawn of E. Coli</li> | ||
+ | <li><a href="#protocols" class="help">Basic Assembly Protocols</li> | ||
</ul> </td> | </ul> </td> | ||
</tr> | </tr> |
Latest revision as of 22:19, 27 October 2010
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