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Team:Chiba/Notebook 3
From 2010.igem.org
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<li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a></li> | <li><a href="https://2010.igem.org/Team:Chiba/Project"><span>Project</span></a></li> | ||
<li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li> | <li><a href="https://2010.igem.org/Team:Chiba/Parts"><span>Parts</span></a></li> | ||
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<li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a></li> | <li><a href="https://2010.igem.org/Team:Chiba/Notebook"><span>Notebook</span></a></li> | ||
<li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li> | <li><a href="https://2010.igem.org/Team:Chiba/Support"><span>Support</span></a></li> | ||
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</ul> | </ul> | ||
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<!--- Main Contents Start ---> | <!--- Main Contents Start ---> | ||
| - | <br><br><br> | + | <br><br><br><br> |
| - | <font size="5">Characterization of | + | <font size="5">Characterization of lux inverter</font><br><br> |
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<html><h3>2010-10-8</h3></html> | <html><h3>2010-10-8</h3></html> | ||
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<pre>transformation | <pre>transformation | ||
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liquid culture | liquid culture | ||
| - | LB (Carb-Kan) 1mL | + | Sample:Plux inv-GFP and Plac-LuxR |
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| + | LB (Carb-Kan,AHL 0 nM and 1000 nM ) 1mL | ||
37℃ 12 h 200 rpm | 37℃ 12 h 200 rpm | ||
</pre> | </pre> | ||
<html><h3>2010-10-26</h3></html> | <html><h3>2010-10-26</h3></html> | ||
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| + | Spin-dawn and observed the pellet | ||
| + | </pre> | ||
Latest revision as of 03:21, 28 October 2010
Characterization of lux inverter
2010-10-8
transformation
Plasmid:BBa_R0061
BBa_E0240
strain:XL10-G
2010-10-9
Mini-prep (Sigma Aldrich)
BBa_R0061
BBa_E0240
digestion
--------------------
BBa_R0061 20 µL
10×Buffer 3 µL
SpeI 1 µL
PstI 1 µL
NFW 5 µL
--------------------
30 µL
--------------------
BBa_E0240 20 µL
10×Buffer 3 µL
XbaI 1 µL
PstI 1 µL
NFW 5 µL
--------------------
30 µL
↓
gel extraction
2010-10-11
Ligation
-----------------------
BBa_R0061 (70 ng) 1 µL
BBa_E0240 (100 ng) 2 µL
ATP (10 mM) 1 µL
10×Buffer 1 µL
T4 DNA Ligase 1 µL
NFW 4 µL
-----------------------
10 µL
↓
transformation
strain:XL10-G
2010-10-12
Ligation
-----------------------
BBa_R0061(100 ng) 1 µL
BBa_E0240 (100 ng) 3 µL
ATP (10 mM) 1 µL
T4 DNA Ligase 1 µL
dH<sub>2</sub>O 4 µL
-----------------------
10 µL
↓
transformation
strain:XL10-Gold
2010-10-21
Double transformation Strain:DH10B Plasmid:Plux inv-GFP & Plac-LuxR
2010-10-22
liquid culture Sample:Plux inv-GFP and Plac-LuxR LB (Carb-Kan,AHL 0 nM and 1000 nM ) 1mL 37℃ 12 h 200 rpm
2010-10-26
Spin-dawn and observed the pellet
