Team:Calgary/Project/Achievements

From 2010.igem.org

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<li><a href="https://2010.igem.org/Team:Calgary/Project/Controls">Testing our system</a></li>
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<li><a href="https://2010.igem.org/Team:Calgary/Project/Controls">Testing Our System</a></li>
<li><a href="https://2010.igem.org/Team:Calgary/Project/Achievements">Achievements</a></li>
<li><a href="https://2010.igem.org/Team:Calgary/Project/Achievements">Achievements</a></li>
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<p>
<p>
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<h2 style="color:#0066CC">Bronze medal requirements</h2>
 
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<li>Register the team</li>
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<h2 style="color:#0066CC">Overview of things completed</h2>
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<li>Successfully complete and submit a project summary form</li>
 
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<li>Create a Wiki which includes all the details of the project</li>
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<table>
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<tr>
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<td>
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<img src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/19-1.png" border="0" alt="Photobucket"></img></td>
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<td>malE, malE31, malEΔSS, ibpAB Fusion Promoter, cpxP Promoter</td>
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</tr>
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</table>
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<li>Present a presentation and a poster at the iGEM Jamboree</li>
 
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<li>Enter information detailing at least one new standard BioBrick Part or Device in the Registry of Parts </li>
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<table>
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<tr>
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<td>
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cpxR reporter, degP reporter, ibpAB reporter, malE generator, malE31 generator, pBad/araC reporters, malE31 generator with cpxR reporter circuit</td>
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<td><img src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/20-1.png" border="0" alt="Photobucket"></img></td>
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</table>
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<li>Data was entered for 11 new biobrick parts</li>
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<td>
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<img src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/21-1-1.png" border="0" alt="Photobucket"></img>
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<td>malE, malE31, ibpAB reporter, cpxR reporter, degP reporter</td>
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</tr>
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</table>
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<h2 style="color:#0066CC">Bronze Medal Requirements</h2>
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<li>Submit DNA for at least one new BioBrick Part or Device to the Registry of Parts</li>
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<table border="1">
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<tr>
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<td><img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /></td>
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<td>Register the team</td>
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</tr>
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<tr>
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<td><img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /></td>
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<td>Successfully complete and submit a project summary form</td>
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</tr>
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<li>We designed and submitted DNA for three new biobrick parts</li>
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<tr>
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<td><img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /></td>
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<td>Create a Wiki which includes all the details of the project</td>
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</tr>
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<ul>
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<tr>
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<li><p>Cytoplasmic maltose binding protein (malESS)</li>
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<td><img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /></td>
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<li>IbpAb/ fxsA fusion promoter</li>
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<td>Present a presentation and a poster at the iGEM Jamboree</td>
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<li> CpxP Promoter </li></ul>
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<ul>
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<tr>
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We also built the following new constructs:
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<td><img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /><br/>Please see our <br/>Parts page <a href="https://2010.igem.org/Team:Calgary/Parts/Parts_Submitted"> here</a>.</td>
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<td>Enter information detailing at least one new standard BioBrick Part or Device in the Registry of Parts</td>
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</tr>
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<li>ibpAB reporter BBa_K339011</li>
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<tr>
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<li>CpxR reporter BBa_K339007</li>
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<td><img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /><br/>Please see our <br/>Parts page <a href="https://2010.igem.org/Team:Calgary/Parts/Parts_Submitted"> here</a>.</td>
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<li>DegP reporter BBa_K339008</li>
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<td>Submit DNA for at least one new BioBrick Part or Device to the Registry of Parts</td>
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<li>MalE generator BBa_K339004</li>
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</tr>
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<li>MalE31 generator BBa_K339005</li>
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</table>
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<li>CpxR Promoter with RFP Reporter and Arabinose Inducible MalE31 BBa_K339009 </li></ul>
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All of these parts were verified via restriction digests and PCR, and then finally sequenced.
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<h2 style="color:#0066CC">Silver Medal Requirements</h2>
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<h2 style="color:#0066CC">Silver medal Requirements</h2>
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<table>
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<tr>
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<td><img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /> </td>
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<td><h3>Demonstrate that one of your parts works as expected</h3></td>
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</tr>
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</table>
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<ul><h3>Demonstrate that one of your parts works as expected</h3>
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<ul>
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<li>Using various methods of characterization, we confirmed that our CpxR reporter circuit was functional. In adition, our maltose binding protein (MalE) and mutant maltose binding protein (MalE31) were shown to be functional. Click <a href="https://2010.igem.org/Team:Calgary/Parts/Characterization"> here</a> for more information.</li>
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<table>
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<tr>
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<td><img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /></td>
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<td><h3>Characterize the operation of at least one new BioBrick Part or Device and enter this information on the Parts or Device page via the Registry of Parts</h3></td>
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</tr>
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</table>
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<li>Through our characterization data, we showed that several of our parts worked as expected. Click here for more information.
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<li>The CpxR reporter, MalE31, and MalE were shown to be functional through characterization. Click <a href="https://2010.igem.org/Team:Calgary/Parts/Parts"> here</a> for more information.</li>
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<p>The CpxR reporter was found  to report on  misfolding protein.  We characterized this promoter with varying concentrations of folding and misfolding proteins produced which we assumed is correlated to arabinose concentrations due to the use of AraC promoter.  We also tested this promoter with NlpE, an outer membrane lipoprotein that literature has found activates the Cpx pathway.  Finally, we tested this promoter in varying temperature to produce a heat shock response.</p></li>
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<h2 style="color:#0066CC">Gold Medal Requirements</h2>
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<h3>Characterize the operation of at least one new BioBrick Part or Device and enter this information on the Parts or Device page via the Registry of Parts</h3>
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<li>Both MalE and MalE31 also worked as expected, malE folding in the periplasm and malE31 showing a misfolding response. This was indicated  through testing with a reporter from another lab (Raivio) (for more info click here) as well as tetsing with our cpxR reporter click here.</li>
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<table>
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<tr>
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<td><img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /></td>
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<td><h3>Characterize or improve an existing BioBrick Part or Device and enter this information back on the Registry</h3></td>
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</tr>
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</table>
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<li>We characterized the cpxR reporter, malE31, MalE and IbpAB.  See characterization data here.</li></ul>
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<ul>
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<li>More characterization was given to the Registry of Standard Parts for the cpxR promoter as well as for the degP promoter.  Details can be found on our characterization page <a href="https://2010.igem.org/Team:Calgary/Parts/Characterization">here</a>. </li>
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</ul>
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<h2 style="color:#0066CC">Gold Medal requirements</h2>
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<table>
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<tr>
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<td> <img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /></td>
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<td><h3>Help another iGEM team by, for example, characterizing a part, debugging a construct, or modeling or simulating their system </h3></td>
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</tr>
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</table>
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<ul><h3>Characterize or improve an existing BioBrick Part or Device and enter this information back on the Registry.</h3>  
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<ul>
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<li>We tested out a part (nms6) for the University of Lethbridge.  We assayed it for possible misfolding in the cytoplasm.  Results can be found <a href="https://2010.igem.org/Team:Calgary/Parts/Characterization"> here</a>.</li>
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<li>we entered new DNA as well as new sequences for malE and malE31.</li>
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<li> We attended an Alberta-wide conference where teams from the University of Calgary, the University of Lethbridge, and the University of Alberta did mock presentations, gave each other future directions, and gave possible troubleshooting methods.</li>
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<li>we entered characterization data for the CpxR promoter.</li>
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<h3>Help another iGEM team by, for example, characterizing a part, debugging a construct, or modeling or simulating their system. </h3>
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<li>We filled out surveys for the University of Warsaw and the University of Edinburgh iGEM teams</li>
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<li>We tested out a part for Lethbridge. We characterized it for possible misfolding in the cytoplasm. Results can be found here.</li>
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<table>
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<tr>
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<td> <img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /></td>
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<td><h3>Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation</h3></td>
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</tr>
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</table>
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<li>We also attended a regional workshop as well as a mock iGEM event with the University of Alberta as well as the University of Lethbridge.  Here we helped each their out with our projects by practicing our presentations for each other, giving suggestions, future directions as well as troubleshooting</li>
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<li>We chose to use current social media in the form of podcasts which covered different ethical issues pertaining to synthetic biology. These are posted <a href="https://2010.igem.org/Team:Calgary/Community/Podcasts">here</a>. In addition to this, an analysis of the social, ethical, and economical factors which our project dealt with was written and is posted <a href="https://2010.igem.org/Team:Calgary/Community/Ethics">here</a></li>
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<li>Finally our team filled out surveys for the following teams:</li>
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<li>This year we chose to approach ethics by making a podscasts covering a few different ethical issues pertaining to synthetic biology. We felt tat this would be a novel way to increase awareness about the field of synthetic biology while having a discussion of important issues. We felt that this would be a much more accessible way for the public to gain a better understanding of what the real fears in synthetic biology is as well as to dispel some of the misleading impressions given by media sources.</li>
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</ul>
</ul>
</p>
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</div>
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Latest revision as of 04:00, 28 October 2010

Project Descriptions

Achievements

Overview of things completed

Photobucket malE, malE31, malEΔSS, ibpAB Fusion Promoter, cpxP Promoter
cpxR reporter, degP reporter, ibpAB reporter, malE generator, malE31 generator, pBad/araC reporters, malE31 generator with cpxR reporter circuit Photobucket
Photobucket malE, malE31, ibpAB reporter, cpxR reporter, degP reporter

Bronze Medal Requirements

Register the team
Successfully complete and submit a project summary form
Create a Wiki which includes all the details of the project
Present a presentation and a poster at the iGEM Jamboree

Please see our
Parts page here.		 Enter information detailing at least one new standard BioBrick Part or Device in the Registry of Parts

Please see our
Parts page here.		 Submit DNA for at least one new BioBrick Part or Device to the Registry of Parts

Silver Medal Requirements

Demonstrate that one of your parts works as expected
  • Using various methods of characterization, we confirmed that our CpxR reporter circuit was functional. In adition, our maltose binding protein (MalE) and mutant maltose binding protein (MalE31) were shown to be functional. Click here for more information.

    • Characterize the operation of at least one new BioBrick Part or Device and enter this information on the Parts or Device page via the Registry of Parts
  • The CpxR reporter, MalE31, and MalE were shown to be functional through characterization. Click here for more information.

    • Gold Medal Requirements

    Characterize or improve an existing BioBrick Part or Device and enter this information back on the Registry
    • More characterization was given to the Registry of Standard Parts for the cpxR promoter as well as for the degP promoter. Details can be found on our characterization page here.

    Help another iGEM team by, for example, characterizing a part, debugging a construct, or modeling or simulating their system

    • We tested out a part (nms6) for the University of Lethbridge. We assayed it for possible misfolding in the cytoplasm. Results can be found here.
    • We attended an Alberta-wide conference where teams from the University of Calgary, the University of Lethbridge, and the University of Alberta did mock presentations, gave each other future directions, and gave possible troubleshooting methods.
    • We filled out surveys for the University of Warsaw and the University of Edinburgh iGEM teams

      • Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation
    • We chose to use current social media in the form of podcasts which covered different ethical issues pertaining to synthetic biology. These are posted here. In addition to this, an analysis of the social, ethical, and economical factors which our project dealt with was written and is posted here

Retrieved from "http://2010.igem.org/Team:Calgary/Project/Achievements"