Team:Calgary/Project/Achievements

From 2010.igem.org

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<li><a href="https://2010.igem.org/Team:Calgary/Project/Controls">Testing our system</a></li>
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<li><a href="https://2010.igem.org/Team:Calgary/Project/Controls">Testing Our System</a></li>
<li><a href="https://2010.igem.org/Team:Calgary/Project/Achievements">Achievements</a></li>
<li><a href="https://2010.igem.org/Team:Calgary/Project/Achievements">Achievements</a></li>
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<p>
<p>
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<h2 style="color:#0066CC">Bronze medal requirements</h2>
 
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<li>Register the team</li>
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<h2 style="color:#0066CC">Overview of things completed</h2>
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<li>Successfully complete and submit a project summary form</li>
 
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<li>Create a Wiki which includes all the details of the project</li>
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<table>
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<tr>
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<td>
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<img src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/19-1.png" border="0" alt="Photobucket"></img></td>
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<td>malE, malE31, malEΔSS, ibpAB Fusion Promoter, cpxP Promoter</td>
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</tr>
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</table>
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<li>Present a presentation and a poster at the iGEM Jamboree</li>
 
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<li>Enter information detailing at least one new standard BioBrick Part or Device in the Registry of Parts </li>
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<table>
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<tr>
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<td>
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cpxR reporter, degP reporter, ibpAB reporter, malE generator, malE31 generator, pBad/araC reporters, malE31 generator with cpxR reporter circuit</td>
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<td><img src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/20-1.png" border="0" alt="Photobucket"></img></td>
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</tr>
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</table>
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<li>Data was entered for 11 new biobrick parts</li>
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<table>
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<tr>
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<td>
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<img src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/21-1-1.png" border="0" alt="Photobucket"></img>
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<td>malE, malE31, ibpAB reporter, cpxR reporter, degP reporter</td>
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</tr>
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</table>
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<h2 style="color:#0066CC">Bronze Medal Requirements</h2>
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<li>Submit DNA for at least one new BioBrick Part or Device to the Registry of Parts</li>
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<table border="1">
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<tr>
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<td><img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /></td>
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<td>Register the team</td>
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</tr>
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<tr>
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<td><img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /></td>
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<td>Successfully complete and submit a project summary form</td>
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</tr>
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<li>We designed and submitted DNA for three new biobrick parts</li>
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<tr>
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<td><img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /></td>
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<td>Create a Wiki which includes all the details of the project</td>
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</tr>
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<li><p>Cytoplasmic maltose binding protein (malESS)</li>
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<tr>
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IbpAb/ fxsA fusion promoter
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<td><img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /></td>
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- cpxP Promoter
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<td>Present a presentation and a poster at the iGEM Jamboree</td>
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</tr>
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We also built the following new constructs:
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<tr>
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<td><img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /><br/>Please see our <br/>Parts page <a href="https://2010.igem.org/Team:Calgary/Parts/Parts_Submitted"> here</a>.</td>
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<td>Enter information detailing at least one new standard BioBrick Part or Device in the Registry of Parts</td>
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</tr>
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-ibpAB reporter
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<tr>
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CpxR reporter
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<td><img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /><br/>Please see our <br/>Parts page <a href="https://2010.igem.org/Team:Calgary/Parts/Parts_Submitted"> here</a>.</td>
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DegP reporter
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<td>Submit DNA for at least one new BioBrick Part or Device to the Registry of Parts</td>
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MalE generator
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</tr>
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MalE31 generator
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</table>
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K13500-I13507-I0500-B0034-malE31
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All of these parts were verified via resriction digests and PCR, and then finally sequenced.
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<h2 style="color:#0066CC">Silver Medal Requirements</h2>
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Silver medal Requirments
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<table>
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<tr>
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<td><img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /> </td>
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<td><h3>Demonstrate that one of your parts works as expected</h3></td>
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</tr>
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</table>
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Demonstrate that one of your parts works as expected
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<ul>
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<li>Using various methods of characterization, we confirmed that our CpxR reporter circuit was functional. In adition, our maltose binding protein (MalE) and mutant maltose binding protein (MalE31) were shown to be functional. Click <a href="https://2010.igem.org/Team:Calgary/Parts/Characterization"> here</a> for more information.</li>
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<table>
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<tr>
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<td><img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /></td>
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<td><h3>Characterize the operation of at least one new BioBrick Part or Device and enter this information on the Parts or Device page via the Registry of Parts</h3></td>
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</tr>
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</table>
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Through our characeroization data, we showed that several of our parts worked as expected.
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<li>The CpxR reporter, MalE31, and MalE were shown to be functional through characterization. Click <a href="https://2010.igem.org/Team:Calgary/Parts/Parts"> here</a> for more information.</li>
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The cpxR reporter was found  to report on  misfolding protein.  We characterized this promoter with varying concentrations of folding and midfolding proteins produced.  We also tested this promoter with NLPE, an outer membrane lipoprotein that literature has found activates the cpx pathway.  Finally, we tested this promoter in varying heat shock conditions.
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<h2 style="color:#0066CC">Gold Medal Requirements</h2>
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Both MalE and malE31 also worked as expected, malE folding in the periplams and malE31 showing a misfolding reponse. This was indictaed  through tetsing with a reporter from another lab (Raivio) (for more info click here) as well as tetsing with our cpxR reporter click here.
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<table>
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<tr>
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<td><img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /></td>
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<td><h3>Characterize or improve an existing BioBrick Part or Device and enter this information back on the Registry</h3></td>
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</tr>
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</table>
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We characetrized the cpxR reporter, malE31 ibpAB and .  See characterization data here.
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<ul>
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<li>More characterization was given to the Registry of Standard Parts for the cpxR promoter as well as for the degP promoter.  Details can be found on our characterization page <a href="https://2010.igem.org/Team:Calgary/Parts/Characterization">here</a>. </li>
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</ul>
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Characterize or improve an existing BioBrick Part or Device and enter this information back on the Registry.
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<table>
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<tr>
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<td> <img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /></td>
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<td><h3>Help another iGEM team by, for example, characterizing a part, debugging a construct, or modeling or simulating their system </h3></td>
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</tr>
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</table>
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-we entered new DNA as well as new sequences for malE and malE31.
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<ul>
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- we entered characterization data for the cpxR promoter
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<li>We tested out a part (nms6) for the University of Lethbridge. We assayed it for possible misfolding in the cytoplasm.  Results can be found <a href="https://2010.igem.org/Team:Calgary/Parts/Characterization"> here</a>.</li>
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Help another iGEM team by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.  
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<li> We attended an Alberta-wide conference where teams from the University of Calgary, the University of Lethbridge, and the University of Alberta did mock presentations, gave each other future directions, and gave possible troubleshooting methods.</li>
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-We tetsed out a part for Lethbridge.  We characterized it for possible misfodling in the cytoplasm.  Resuylts can be found here.
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<li>We filled out surveys for the University of Warsaw and the University of Edinburgh iGEM teams</li>
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- We also attended a regional workship as well as a mock Igem event with the Univeristy of Salberta as well as the Univeristy of Lethbridge.  Here we helped each their out with our projects by practicing our presentaitons for each other, giving suggestions, future directions as well as touble shooting
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<table>
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<tr>
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<td> <img style="height:50px; width:50px;" src="http://i872.photobucket.com/albums/ab287/iGEMCalgary_2010/Checkmark.png" /></td>
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<td><h3>Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation</h3></td>
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</tr>
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</table>
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-Finally our team filled out surveys for the following teams:
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<li>We chose to use current social media in the form of podcasts which covered different ethical issues pertaining to synthetic biology. These are posted <a href="https://2010.igem.org/Team:Calgary/Community/Podcasts">here</a>. In addition to this, an analysis of the social, ethical, and economical factors which our project dealt with was written and is posted <a href="https://2010.igem.org/Team:Calgary/Community/Ethics">here</a></li>
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</ul>
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</p>
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- This year we chose to approach ethjcs by making a pods acts covering a few different ethical issues pertaining to synthetic biology.  We felt tat this would be a novel way to increase awareness about the field of syntyehtic biology while having a discussion of important issues.  We felt that this would be a much more accessible way for the public to gain a better understanding of what the real fears in sythetic biology is as well as to dispell some of the misleading iompressions given by media sources.
 
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</p>
 
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Latest revision as of 04:00, 28 October 2010

Project Descriptions

Achievements

Overview of things completed

Photobucket malE, malE31, malEΔSS, ibpAB Fusion Promoter, cpxP Promoter
cpxR reporter, degP reporter, ibpAB reporter, malE generator, malE31 generator, pBad/araC reporters, malE31 generator with cpxR reporter circuit Photobucket
Photobucket malE, malE31, ibpAB reporter, cpxR reporter, degP reporter

Bronze Medal Requirements

Register the team
Successfully complete and submit a project summary form
Create a Wiki which includes all the details of the project
Present a presentation and a poster at the iGEM Jamboree

Please see our
Parts page here.		 Enter information detailing at least one new standard BioBrick Part or Device in the Registry of Parts

Please see our
Parts page here.		 Submit DNA for at least one new BioBrick Part or Device to the Registry of Parts

Silver Medal Requirements

Demonstrate that one of your parts works as expected
  • Using various methods of characterization, we confirmed that our CpxR reporter circuit was functional. In adition, our maltose binding protein (MalE) and mutant maltose binding protein (MalE31) were shown to be functional. Click here for more information.

    • Characterize the operation of at least one new BioBrick Part or Device and enter this information on the Parts or Device page via the Registry of Parts
  • The CpxR reporter, MalE31, and MalE were shown to be functional through characterization. Click here for more information.

    • Gold Medal Requirements

    Characterize or improve an existing BioBrick Part or Device and enter this information back on the Registry
    • More characterization was given to the Registry of Standard Parts for the cpxR promoter as well as for the degP promoter. Details can be found on our characterization page here.

    Help another iGEM team by, for example, characterizing a part, debugging a construct, or modeling or simulating their system

    • We tested out a part (nms6) for the University of Lethbridge. We assayed it for possible misfolding in the cytoplasm. Results can be found here.
    • We attended an Alberta-wide conference where teams from the University of Calgary, the University of Lethbridge, and the University of Alberta did mock presentations, gave each other future directions, and gave possible troubleshooting methods.
    • We filled out surveys for the University of Warsaw and the University of Edinburgh iGEM teams

      • Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation
    • We chose to use current social media in the form of podcasts which covered different ethical issues pertaining to synthetic biology. These are posted here. In addition to this, an analysis of the social, ethical, and economical factors which our project dealt with was written and is posted here

Retrieved from "http://2010.igem.org/Team:Calgary/Project/Achievements"