Team:Panama/22 September 2010
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- | '''Digestion of terminator''' | + | '''Digestion of terminator (This has nothing to do with Arnold's peristaltic movements)''' |
- | Today we did the plasmid terminator digestion with the | + | |
+ | Today we did the plasmid terminator digestion with the Xba1 and Pst1 enzyme. | ||
[[Image:Cuadro_22_de_sep.jpg|200px|thumb|left|alt text]] | [[Image:Cuadro_22_de_sep.jpg|200px|thumb|left|alt text]] | ||
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- | 3 hours at 37°C | + | 3 hours at 37°C and 20 minutes at 80°C disactivation. |
- | 20 minutes at 80°C disactivation | + | |
Agarose Gel 1% | Agarose Gel 1% | ||
- | [[Image:GEL22SEPTIEMBRE.JPG| | + | [[Image:Cuadro22SEPTIEMBRE.JPG|300px|thumb|left|alt text]] |
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+ | [[Image:GEL22SEPTIEMBRE.JPG|300px|thumb|left|alt text]] | ||
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Size of insert: 151 bp | Size of insert: 151 bp | ||
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Size of Plasmid: 3168 bp | Size of Plasmid: 3168 bp | ||
{{:Team:Panama/calendar2}} | {{:Team:Panama/calendar2}} |
Latest revision as of 03:25, 28 October 2010
September 22
Digestion of terminator (This has nothing to do with Arnold's peristaltic movements)
Today we did the plasmid terminator digestion with the Xba1 and Pst1 enzyme.
3 hours at 37°C and 20 minutes at 80°C disactivation.
Agarose Gel 1%
Size of insert: 151 bp
Size of Plasmid: 3168 bp
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