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Team:UPO-Sevilla/Notebook/08 03
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<h2>Production Team</h2> | <h2>Production Team</h2> | ||
| - | <p | + | <p>We digest <i>fecI-fecR</i> with PstI and EcoRI restriction enzymes , and run an electrophoresis. We purified from electrophoresis gel (1'5 pb) and ligate it with the vector C3L (chloramphenicol).</p> |
<h2>Assembly Team</h2> | <h2>Assembly Team</h2> | ||
Latest revision as of 14:45, 27 October 2010
August, 3rd
Production Team
We digest fecI-fecR with PstI and EcoRI restriction enzymes , and run an electrophoresis. We purified from electrophoresis gel (1'5 pb) and ligate it with the vector C3L (chloramphenicol).
Assembly Team
We analyzed several candidates from 2+4, 2+5 and 2+7 plates by colony PCR. We had positive candidates of UPO2+4 and UPO2+5. We set up inocula of these candidates and spread on LB+Tc plates.
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