Team:Newcastle/3 September 2010

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(Levan glue)
 
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=Transformation of ''Bacillius subtilis'' 168 with pGFP-rrnB containing filamentous cell part=
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=Levan glue=
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==Aim==
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We started working on levan. For this we used agar plates containing 10% sucrose and ''Bacillus subtilis'' 168 was grown onto that medium. The sucrose cultures and controls were compared. The cultures grown on sucrose should be considerably thicker.  
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The aim of the experiment is to transform ''Bacillus subtilis'' 168 with the plasmid pGFP-rrnB containing our filamentous cell part. After transformation the plasmid will integrate into the chromosome at ''amyE''. This integration removes the ability to break down starch, allowing us to select for our desired construct using the iodine test.
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The overnight cultures of the different strains were also grown on solid agar plates with and without sucrose.
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For more information levan, please refer to: [[Team:Newcastle/glue|levan]].
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[[Image:filamentous_in_pgfprrnb.jpg|800px]]
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[[Image:DSC01022.JPG|500px|''B. subtilis producing levan]]
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==Materials and Protocol==
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'''Image 1''': Image showing ''B. subtilis'' producing levan on agar plate containing 10% sucrose.
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Please refer to: [[Team:Newcastle/Transformation of B. subtilis| Transformation of ''Bacillus subtilis'']]
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Note: Overnigth culture of ''B. subtilis'' 168 in MM competence medium was done the day before and the iodine test was performed the day after transformation.
 
{{Team:Newcastle/footer}}
{{Team:Newcastle/footer}}

Latest revision as of 02:08, 28 October 2010

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Levan glue

We started working on levan. For this we used agar plates containing 10% sucrose and Bacillus subtilis 168 was grown onto that medium. The sucrose cultures and controls were compared. The cultures grown on sucrose should be considerably thicker.

The overnight cultures of the different strains were also grown on solid agar plates with and without sucrose. For more information levan, please refer to: levan.

B. subtilis producing levan

Image 1: Image showing B. subtilis producing levan on agar plate containing 10% sucrose.


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